https://eln.excellgen.com/index.php?action=history&feed=atom&title=MS_6_8_2017MS 6 8 2017 - Revision history2026-10-18T10:26:43ZRevision history for this page on the wikiMediaWiki 1.44.0https://eln.excellgen.com/index.php?title=MS_6_8_2017&diff=15999&oldid=prevCrm at 13:58, 9 June 20172017-06-09T13:58:56Z<p></p>
<p><b>New page</b></p><div>Culture 1483 T4 DNA Ligase 200ml.<br />
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Culture 1498 Taq DNA Ligase 8L, induce with IPTG.<br />
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Spin down 1497 sso7d-ToughTaq.<br />
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1946 TdT bovine homogenize, centrifugate and load onto Ni column.<br />
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1942 phi 29, 1601 6His-GST-i3C, 1518 T3 Ligase, 1765 T4 DNA Polymerase pick up single colony each to culture in LB/K media for glycerol stock.<br />
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1942 phi 29, 1601 6His-GST-i3C, 1518 T3 Ligase, 1765 T4 DNA Polymerase, transfer into Nico21, culture on LB/K/C agar over night.<br />
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Elute QHP column (load with 1464 T7 DNA Ligase)<br />
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[[File:126-1464 T7 DNA Ligase QHP 6 8 2017.jpg|RTENOTITLE]]<br />
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Elute SP FF column (load with 1708 Cre Recombinase) , combine fration with OD<sub>600</sub>>2.5, dialysis through dialysis bag against Cre dialysis buffer.<br />
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Prepare Storage buffer, SP Wash Buffer, SP Elution Buffer.<br />
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Lable transfection reagent.<br />
[[Category:My ELN]]</div>Crm