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MS 7 11 2017 - Revision history
2026-10-18T04:04:51Z
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Crm at 20:17, 13 July 2017
2017-07-13T20:17:17Z
<p></p>
<p><b>New page</b></p><div>Homogenize 818 pHis-GST-His-Flag-His<span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">, centrifugate and load onto Ni column.</span><br />
<br />
<span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">Wash Ni column (load with 1782 Tth-Ligase) with Ni washing buffer/ Tris-HCl/ Buffer B.</span> Elute&nbsp;<span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">1782 Tth-Ligase</span> with Buffer B 07/11/2017<br />
<br />
{| style="width: 500px" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| -0.26<br/><br />
| -0.27<br/><br />
| -0.28<br/><br />
| -0.19<br/><br />
| 0.03<br/><br />
| (1~5)<br/><br />
|-<br />
| 4.68<br/><br />
| 6.36<br/><br />
| 7.35<br/><br />
| 5.35<br/><br />
| 4.74<br/><br />
| (6~10)<br/><br />
|-<br />
| 4.15<br/><br />
| 3.85<br/><br />
| 3.53<br/><br />
| 2.75<br/><br />
| 2.35<br/><br />
| (11~15)<br/><br />
|-<br />
| 1.85<br/><br />
| 1.53<br/><br />
| 1.21<br/><br />
| 0.54<br/><br />
| 0.23<br/><br />
| (16~20)<br/><br />
|-<br />
| 0.12<br/><br />
| 0.08<br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| (21~25)<br/><br />
|}<br />
<br />
collect 6~20.<br />
<br />
<br />
<br />
<span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">Load 423 DeepVent-sso7d onto QHP column.</span><br />
<br />
<br />
<br />
Elute 1567 T5-Exo<br />
<br />
[[File:140-1567 T5-Exo QHP 7 11 2017.jpg|RTENOTITLE]]<br />
<br />
Run SDS-PAGE gel of 1567 T5-Exo 5.55 / 34.5&nbsp;&nbsp;&nbsp; 07/11/2017<br />
<br />
{| style="width:500px" width="500" cellspacing="1" cellpadding="0" border="1"<br />
|-<br />
| <br />
Number<br />
<br />
| <br />
1<br />
<br />
| <br />
2<br />
<br />
| <br />
3<br />
<br />
| <br />
4<br />
<br />
| style="width:33px" | <br />
5<br />
<br />
| style="width:33px" | <br />
6<br />
<br />
| <br />
7<br />
<br />
| <br />
8<br />
<br />
| <br />
9<br />
<br />
| <br />
10<br />
<br />
| <br />
11<br />
<br />
| <br />
12<br />
<br />
|-<br />
| <br />
sample<br />
<br />
| <br />
19<br />
<br />
| <br />
20<br />
<br />
| <br />
21<br />
<br />
| <br />
22<br />
<br />
| style="width:33px" | <br />
23<br />
<br />
| style="width:33px" | <br />
24<br />
<br />
| <br />
25<br />
<br />
| <br />
MW<br />
<br />
| <br />
26<br />
<br />
| <br />
27<br />
<br />
| <br />
28<br />
<br />
| <br />
30<br />
<br />
|-<br />
| <br />
uL<br />
<br />
| <br />
9.1<br />
<br />
| <br />
5.3<br />
<br />
| <br />
3.9<br />
<br />
| <br />
3.0<br />
<br />
| style="width:33px" | <br />
3.1<br />
<br />
| style="width:33px" | <br />
2.6<br />
<br />
| <br />
2.4<br />
<br />
| <br />
10<br />
<br />
| <br />
3.3<br />
<br />
| <br />
4.8<br />
<br />
| <br />
5.3<br />
<br />
| <br />
9.1<br />
<br />
|-<br />
| <br />
ddH<sub>2</sub>O<br />
<br />
| <br />
5<br />
<br />
| <br />
10<br />
<br />
| <br />
10<br />
<br />
| <br />
10<br />
<br />
| style="width:33px" | <br />
10<br />
<br />
| style="width:33px" | <br />
10<br />
<br />
| <br />
10<br />
<br />
| <br />
\<br />
<br />
| <br />
10<br />
<br />
| <br />
10<br />
<br />
| <br />
10<br />
<br />
| <br />
5<br />
<br />
|}<br />
<br />
&nbsp;[[File:Protein Expression SDS-PAGE 140-1567 T5-Exo QHP 7 11 2017.jpg|RTENOTITLE]]<br />
<br />
Forward lane 11~12 to the next step.<br />
[[Category:My ELN]]</div>
Crm