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MS 7 12 2017 - Revision history
2026-10-18T04:04:34Z
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Crm at 20:17, 13 July 2017
2017-07-13T20:17:03Z
<p></p>
<p><b>New page</b></p><div><span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">Wash Ni column (load with </span>818 pHis-GST-His-Flag-His) with Ni washing Buffer/ Tris-HCl/ Buffer B<br />
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Elute&nbsp;818 pHis-GST-His-Flag-His with Buffer B 07/12/2017<br />
<br />
{| style="width: 500px" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| -0.30<br/><br />
| -0.26<br/><br />
| 0.05<br/><br />
| 1.95<br/><br />
| 2.26<br/><br />
| (6~10)<br/><br />
|-<br />
| 1.68<br/><br />
| 1.18<br/><br />
| 0.85<br/><br />
| 0.62<br/><br />
| 0.43<br/><br />
| (11~15)<br/><br />
|-<br />
| 0.30<br/><br />
| 0.20<br/><br />
| 0.14<br/><br />
| 0.08<br/><br />
| 0.05<br/><br />
| (16~20)<br/><br />
|}<br />
<br />
collect 9~16.<br />
<br />
<br />
<br />
Load <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">1782 Tth-Ligase onto QHP column.</span><br />
<br />
<br />
<br />
Elute <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">423 DeepVent-sso7d from QHP column.</span><br />
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<span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">[[File:141-423 DeepVent-sso7d QHP 7 12 2017.jpg|RTENOTITLE]]</span><br />
<br />
<br />
<br />
<span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">Run SDS-PAGE gel of </span>423 DeepVent-sso7d &nbsp;8.69 / 99.5&nbsp;&nbsp;&nbsp; 07/12/2017<br />
<br />
{| style="width:500px" width="500" cellspacing="1" cellpadding="0" border="1"<br />
|-<br />
| <br />
Number<br />
<br />
| <br />
1<br />
<br />
| <br />
2<br />
<br />
| <br />
3<br />
<br />
| <br />
4<br />
<br />
| style="width:33px" | <br />
5<br />
<br />
| style="width:33px" | <br />
6<br />
<br />
| <br />
7<br />
<br />
| <br />
8<br />
<br />
|-<br />
| <br />
sample<br />
<br />
| <br />
14<br />
<br />
| <br />
15<br />
<br />
| <br />
16<br />
<br />
| <br />
17<br />
<br />
| style="width:33px" | <br />
18<br />
<br />
| style="width:33px" | <br />
MW<br />
<br />
| <br />
20<br />
<br />
| <br />
22<br />
<br />
|-<br />
| <br />
uL<br />
<br />
| <br />
15<br />
<br />
| <br />
4.6<br />
<br />
| <br />
2.7<br />
<br />
| <br />
2.7<br />
<br />
| style="width:33px" | <br />
4.0<br />
<br />
| style="width:33px" | <br />
10<br />
<br />
| <br />
5.0<br />
<br />
| <br />
5.9<br />
<br />
|-<br />
| <br />
ddH<sub>2</sub>O<br />
<br />
| <br />
\<br />
<br />
| <br />
10<br />
<br />
| <br />
10<br />
<br />
| <br />
10<br />
<br />
| style="width:33px" | <br />
10<br />
<br />
| style="width:33px" | <br />
\<br />
<br />
| <br />
10<br />
<br />
| <br />
10<br />
<br />
|}<br />
<br />
&nbsp;[[File:Protein Expression SDS-PAGE 141-423 DeepVent-sso7d QHP 7 12 2017.jpg|RTENOTITLE]]<br />
<br />
Forward lane 1~2 to the next step.<br />
<br />
Dialysis of 1567 T5-Exo. 1.5mg/ml, 8.0ml.<br />
[[Category:My ELN]]</div>
Crm