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MS 7 13 2017 - Revision history
2026-10-18T04:04:40Z
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Crm at 20:16, 13 July 2017
2017-07-13T20:16:24Z
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<p><b>New page</b></p><div>Load 818 pHis-GST-His-Flag-His <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">onto QHP column.</span><br />
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Elute <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">1782 Tth-Ligase from QHP column.</span><br />
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<span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">[[File:142-1782 Tth ligase QHP 7 13 2017.jpg]]</span><br />
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<span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">Run SDS-PAGE gel of </span>1782 Tth-Ligase &nbsp;&nbsp;6.56 / 79.1&nbsp;&nbsp;&nbsp; 07/13/2017<br />
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{| style="width:500px" width="500" cellspacing="1" cellpadding="0" border="1"<br />
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Number<br />
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1<br />
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2<br />
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3<br />
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4<br />
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5<br />
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6<br />
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7<br />
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sample<br />
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MW<br />
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FT1<br />
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14<br />
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17<br />
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18<br />
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23<br />
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FT2<br />
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uL<br />
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10<br />
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15<br />
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10<br />
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0.5<br />
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0.22<br />
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0.9<br />
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15<br />
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ddH<sub>2</sub>O<br />
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\<br />
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\<br />
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5<br />
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15<br />
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15<br />
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15<br />
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\<br />
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|}<br />
<span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none"></span><br />
FT1: flow through after Ni column.<br />
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FT2: flow through after QHP column.<br />
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[[File:Protein Expression SDS-PAGE 142-1782 Tth ligase QHP 7 13 2017.jpg]]<br />
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Forward lane 5~6 to the next step.<br />
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Dialysis of 423 <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">DeepVent-sso7d</span>. 1.50mg/ml, 8.0ml.<br />
[[Category:My ELN]]</div>
Crm