Crm at 14:51, 6 July 2017

2017-07-06T14:51:26Z

New page

Culture 423 <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">pET30b-DeepVent-sso7d 200ml. Culture 1567 Exo 8L, induced with IPTG.

Homogenize 2462 Tn5 transposase, load onto Ni column.

Load 1995 Kfu onto QHP column.

Wash Ni column (load with 1811 vPol) with Ni washing buffer/ Tris-HCl/ Buffer B.

Elute 1811 vPol with Buffer B 07/04/2017

{| style="width: 500px" cellspacing="1" cellpadding="1" border="1"
|-
| -0.02 
| -0.11 
| -0.14 
| -0.21 
| -0.08 
| (1~5) 
|-
| 1.18 
| 3.41 
| 6.35 
| 17.97 
| 36.35 
| (6~10) 
|-
| 20.81 
| 15.44 
| 10.71 
| 4.83 
| 1.61 
| (11~15) 
|-
| 0.76 
| 0.32 
| 0.24 
| 0.12 
| 0.08 
| (16~20) 
|}

collect 6~17.

Elute 1458 KOD

[[File:136-1458 KOD QHP 7 4 2017.jpg|RTENOTITLE]]

Run SDS-PAGE gel of 1458 KOD  7.91/92.2   07/04/2017

{| style="width:500px" width="500" cellspacing="1" cellpadding="0" border="1"
|-
|
Number

|
1

|
2

|
3

|
4

|
5

|
6

|
7

|
8

|
9

|
10

|
11

|
12

|-
|
sample

|
16

|
18

|
20

|
22

|
24

|
26

|
28

|
30

|
MW

|
32

|
34

|
36

|-
|
uL

|
10

|
0.2

|
0.1

|
0.13

|
0.13

|
0.2

|
0.23

|
0.7

|
10

|
2.0

|
4.2

|
7.7

|-
|
ddH2O

|
5

|
15

|
15

|
15

|
15

|
15

|
15

|
15

|
\

|
15

|
10

|
5

|}

[[File:Protein Expression SDS-PAGE 136-1458 KOD QHP 7 4 2017.jpg|RTENOTITLE]]

forward lane 1~3 to the next step.  
[[Category:My ELN]]

MS 7 4 2017 - Revision history

Crm at 14:51, 6 July 2017