Crm at 13:17, 7 July 2017

2017-07-07T13:17:15Z

New page

Culture 1782 <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">Tth Ligase 200ml. Culture 423 <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">pET30b-DeepVent-sso7d 8L, induced with IPTG.

Homogenize 1567 Exo, load onto Ni column.

Load 1811 vPol onto QHP column.

Wash Ni column (load with 2462 Tn5 transposase) with Ni washing buffer/ Tris-HCl/ Buffer B.

Elute 2462 Tn5 transposase with Buffer B 07/05/2017

{| style="width: 500px" cellspacing="1" cellpadding="1" border="1"
|-
| -0.24 
| -0.26 
| -0.24 
| -0.23 
| -0.20 
| (6~10) 
|-
| -0.15 
| 0.28 
| 1.31 
| 0.38 
| 0.04 
| (11~15) 
|-
| 0.00 
| 
| 
| 
| 
| (16~20) 
|}

collect 12~15.

Elute 1995 Kfu

[[File:137-1995 Kfu QHP 7 5 2017.jpg|RTENOTITLE]]

Run SDS-PAGE gel of 1995 Kfu 7.87 / 99.3     07/05/2017

{| style="width:500px" width="500" cellspacing="1" cellpadding="0" border="1"
|-
|
Number

|
1

|
2

|
3

|
4

| style="width:33px" |
5

| style="width:33px" |
6

|
7

|
8

|
9

|
10

|
11

|
12

|-
|
sample

|
17

|
18

|
19

|
20

| style="width:33px" |
21

| style="width:33px" |
22

|
23

|
MW

|
25

|
27

|
29

|
31

|-
|
uL

|
9.1

|
1.5

|
0.8

|
0.7

| style="width:33px" |
1.1

| style="width:33px" |
1.7

|
2.4

|
10

|
3.3

|
4.4

|
6.7

|
11.1

|-
|
ddH2O

|
5

|
15

|
15

|
15

| style="width:33px" |
15

| style="width:33px" |
15

|
15

|
\

|
10

|
10

|
10

|
5

|}

[[File:Protein Expression SDS-PAGE 137-1995 Kfu QHP 7 5 2017.jpg|RTENOTITLE]]

forward lane 1~4 to the next step.  

Dialysis of 1458 KOD. 14.27mg/ml, 4.5ml, purity > 90%
[[Category:My ELN]]

MS 7 5 2017 - Revision history

Crm at 13:17, 7 July 2017