Crm at 13:16, 7 July 2017

2017-07-07T13:16:58Z

New page

Culture 1782 <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">Tth Ligase 8L, induced with IPTG.

Homogenize 423 <span style="color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none">pET30b-DeepVent-sso7d, load onto Ni column.

Load 2462 Tn5 transposase onto QHP column.

Wash Ni column (load with 1567 Exo) with Ni washing buffer/ Tris-HCl/ Buffer B.

Elute 1567 Exo with Buffer B 07/06/2017

{| style="width: 500px" cellspacing="1" cellpadding="1" border="1"
|-
| -0.25 
| -0.26 
| -0.25 
| -0.25 
| -0.23 
| (1~5) 
|-
| -0.10 
| 1.82 
| 1.04 
| 0.36 
| 0.15 
| (6~10) 
|-
| 0.07 
| 0.04 
| 0.03 
| 0.01 
| 0.01 
| (11~15) 
|}

collect 7~11.

Elute 1811 vPol

[[File:138-1811 vPol QHP 7 6 2017.jpg|RTENOTITLE]]

Run SDS-PAGE gel of 1811 vPol 6.63 / 69.2     07/06/2017

{| style="width:500px" width="500" cellspacing="1" cellpadding="0" border="1"
|-
|
Number

|
1

|
2

|
3

|
4

| style="width:33px" |
5

| style="width:33px" |
6

|
7

|
8

|
9

|
10

|
11

|
12

|-
|
sample

|
8

|
10

|
12

|
14

| style="width:33px" |
15

| style="width:33px" |
16

|
17

|
MW

|
18

|
19

|
20

|
21

|-
|
uL

|
5.9

|
3.9

|
4.0

|
2.1

| style="width:33px" |
0.23

| style="width:33px" |
0.38

|
0.3

|
10

|
0.44

|
0.55

|
0.7

|
0.1

|-
|
ddH2O

|
10

|
10

|
10

|
15

| style="width:33px" |
15

| style="width:33px" |
15

|
15

|
\

|
15

|
15

|
15

|
15

|}

[[File:Protein Expression SDS-PAGE 138-1811 vPol QHP 7 6 2017.jpg|RTENOTITLE]]

forward lane 1~7 to the next step.  

Dialysis of 1995 Kfu. 4.18mg/ml, 4.5ml, purity > 90%
[[Category:My ELN]]

MS 7 6 2017 - Revision history

Crm at 13:16, 7 July 2017