https://eln.excellgen.com/index.php?action=history&feed=atom&title=MS_7_7_2017 MS 7 7 2017 - Revision history 2026-10-18T07:59:39Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=MS_7_7_2017&diff=16304&oldid=prev Crm at 19:52, 7 July 2017 2017-07-07T19:52:35Z <p></p> <p><b>New page</b></p><div>Wash Ni column (load with 423 &lt;span style=&quot;color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none&quot;&gt;DeepVent-sso7d&lt;/span&gt;) with Ni washing buffer/ Tris-HCl/ Buffer B.<br /> <br /> Elute&amp;nbsp;423 &lt;span style=&quot;color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; background-color: rgb(255, 255, 255); text-decoration-style: initial; text-decoration-color: initial; display: inline !important; float: none&quot;&gt;DeepVent-sso7d&lt;/span&gt; with Buffer B 07/07/2017<br /> <br /> {| style=&quot;width: 500px&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | -0.29&lt;br/&gt;<br /> | -0.26&lt;br/&gt;<br /> | -0.14&lt;br/&gt;<br /> | 0.99&lt;br/&gt;<br /> | 1.27&lt;br/&gt;<br /> | (6~10)&lt;br/&gt;<br /> |-<br /> | 0.94&lt;br/&gt;<br /> | 0.80&lt;br/&gt;<br /> | 0.70&lt;br/&gt;<br /> | 0.64&lt;br/&gt;<br /> | 0.61&lt;br/&gt;<br /> | (11~15)&lt;br/&gt;<br /> |-<br /> | 0.57&lt;br/&gt;<br /> | 0.50&lt;br/&gt;<br /> | 0.49&lt;br/&gt;<br /> | 0.44&lt;br/&gt;<br /> | 0.40&lt;br/&gt;<br /> | (16~20)&lt;br/&gt;<br /> |-<br /> | 0.39&lt;br/&gt;<br /> | 0.37&lt;br/&gt;<br /> | 0.30&lt;br/&gt;<br /> | 0.28&lt;br/&gt;<br /> | 0.24&lt;br/&gt;<br /> | (21~25)&lt;br/&gt;<br /> |-<br /> | 0.22&lt;br/&gt;<br /> | 0.22&lt;br/&gt;<br /> | 0.16&lt;br/&gt;<br /> | 0.11&lt;br/&gt;<br /> | 0.08&lt;br/&gt;<br /> | (26~30)&lt;br/&gt;<br /> |}<br /> <br /> collect 9~27.<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> Elute 2462 Tn5 transposase<br /> <br /> [[File:139-2462 Tn5 transposase QHP 7 7 2017.jpg|RTENOTITLE]]<br /> <br /> <br /> <br /> Run SDS-PAGE gel of 2462 Tn5 transposase &amp;nbsp;8.41 / 83.8&amp;nbsp;&amp;nbsp;&amp;nbsp; 07/07/2017<br /> <br /> {| style=&quot;width:500px&quot; width=&quot;500&quot; cellspacing=&quot;1&quot; cellpadding=&quot;0&quot; border=&quot;1&quot;<br /> |-<br /> | <br /> Number<br /> <br /> | <br /> 1<br /> <br /> | <br /> 2<br /> <br /> | style=&quot;width:33px&quot; | <br /> 3<br /> <br /> | style=&quot;width:33px&quot; | <br /> 4<br /> <br /> | <br /> 5<br /> <br /> | <br /> 6<br /> <br /> | <br /> 7<br /> <br /> | <br /> 8<br /> <br /> | <br /> 9<br /> <br /> | <br /> 10<br /> <br /> |-<br /> | <br /> sample<br /> <br /> | <br /> 22<br /> <br /> | <br /> 23<br /> <br /> | style=&quot;width:33px&quot; | <br /> 24<br /> <br /> | style=&quot;width:33px&quot; | <br /> 25<br /> <br /> | <br /> 26<br /> <br /> | <br /> MW<br /> <br /> | <br /> 27<br /> <br /> | <br /> 28<br /> <br /> | <br /> 29<br /> <br /> | <br /> 30<br /> <br /> |-<br /> | <br /> uL<br /> <br /> | <br /> 6.3<br /> <br /> | <br /> 8.4<br /> <br /> | style=&quot;width:33px&quot; | <br /> 7.2<br /> <br /> | style=&quot;width:33px&quot; | <br /> 5.6<br /> <br /> | <br /> 5.3<br /> <br /> | <br /> 10<br /> <br /> | <br /> 6.3<br /> <br /> | <br /> 7.2<br /> <br /> | <br /> 8.4<br /> <br /> | <br /> 10<br /> <br /> |-<br /> | <br /> ddH&lt;sub&gt;2&lt;/sub&gt;O<br /> <br /> | <br /> 10<br /> <br /> | <br /> 5<br /> <br /> | style=&quot;width:33px&quot; | <br /> 5<br /> <br /> | style=&quot;width:33px&quot; | <br /> 10<br /> <br /> | <br /> 10<br /> <br /> | <br /> \<br /> <br /> | <br /> 10<br /> <br /> | <br /> 5<br /> <br /> | <br /> 5<br /> <br /> | <br /> 5<br /> <br /> |}<br /> <br /> <br /> <br /> <br /> <br /> [[File:Protein Expression SDS-PAGE 139-2462 Tn5 transposase QHP 7 7 2017.jpg|RTENOTITLE]]<br /> <br /> forward lane 1~4 to the next step. &amp;nbsp;<br /> <br /> <br /> <br /> <br /> <br /> Dialysis of 1811 vPol. 36.0mg/ml, 10.0ml, purity &gt; 90%<br /> [[Category:My ELN]]</div> Crm