https://eln.excellgen.com/index.php?action=history&feed=atom&title=Mini_prep_vacuumMini prep vacuum - Revision history2026-10-18T20:05:06ZRevision history for this page on the wikiMediaWiki 1.44.0https://eln.excellgen.com/index.php?title=Mini_prep_vacuum&diff=20895&oldid=prevPaul.xu at 19:00, 11 June 20202020-06-11T19:00:11Z<p></p>
<p><b>New page</b></p><div>Mini Prep Protocol:&nbsp;<br />
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1) Put samples in the centrifuge for 5 minutes<br />
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2) Dump the liquid out<br />
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3) Add 250 microliters of P1 to every sample and vortex until it is all resuspended<br />
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4) Add 250 microliters of P2 to every sample (if P2 has particles inside, microwave the container)<br />
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5) Add 350 microliters of N3 to every sample<br />
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6) Transfer to a small capped tube and centrifuge for 7 minutes on max speed<br />
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7) Pour the liquid from them into mini columns and place those on&nbsp;vacuum spots (seal vacuum spots which are not in use)<br />
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8) Wait for that to drain and then add Buffer PB to the bottom of the top edge of the column and drain.&nbsp;<br />
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9) Repeat with Buffer PE, twice<br />
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10) Put columns in the used small capped tube and spin for 1 min max speed<br />
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11) Put columns into new capped tubes and put 50 microliters of Elution buffer in to every one.&nbsp;<br />
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12) Centrifuge for one minute, max speed<br />
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13) Use nanodrop and record concentration</div>Paul.xu