https://eln.excellgen.com/index.php?action=history&feed=atom&title=Multiple_clones
Multiple clones - Revision history
2026-10-18T08:38:41Z
Revision history for this page on the wiki
MediaWiki 1.44.0
https://eln.excellgen.com/index.php?title=Multiple_clones&diff=1531&oldid=prev
Hylei at 19:35, 18 August 2011
2011-08-18T19:35:16Z
<p></p>
<p><b>New page</b></p><div>== '''Subcloning of egfp, rfp, mnatue from pet 28b to pLenti and paav vector''' ==<br />
<br />
'''Purpose:''' Subcloning of egfp, rfp, mnatetune, ifp from pet 28b to pLenti and paav vector . <br />
<br />
'''Date: '''2011-8-18 <br />
<br />
'''Notebook:''' Haiyan<br />
<br />
'''Page:'''003025<br />
<br />
'''Clone ID:''' pLenti-IFP (226), pLenti-RFP (221),plenti-EGFP (344), plenti -mnatetuen (345), pLenti-BFP (223), paav-mnatetuen (346), pAAV-CMV-IFP (285),pAAV-rfp (347), paav-egfp (348)<br />
<br />
'''Experimenters:''' Haiyan<br />
<br />
== '''Results''' ==<br />
<br />
=== Subcloning ===<br />
<br />
Date: 2011-8-18 <br />
<br />
Digest fragments from pet 28b vector using bamhi+xhoi <br> <br />
<br />
Digest paav-mcs or plentiplus with bamhi+xhoi <br> <br />
<br />
cut out insert and vector, ligate and transform STBL3 <br />
<br />
=== Colony Screening ===<br />
<br />
Date: 2011-8-18<br> <br />
<br />
Make miniprep and digest with bamhi+xhoi<br> <br />
<br />
[[Image:0818-1.jpg]] <br />
<br />
Lane 1-4: pLenti-IFP (226) clone 1-4, bamhi+xhoi <br> <br />
<br />
Lane 5-8: plenti -mnatetuen (345), clone 1-4, bamhi+xhoi<br> <br />
<br />
Lane 9-12: pLenti-RFP (221), clone 1-4, bamhi+xhoi<br> <br />
<br />
Lane 13-16: pLenti-BFP (223), clone 1-4, bamhi+xhoi<br> <br />
<br />
[[Image:IMG_1274.JPG]] <br />
<br />
Lane 2-5: plenti-EGFP (344) clone 1-4, bamhi+xhoi <br> <br />
<br />
Lane 6-9: paav-mnatetuen (346), clone 1-4, bamhi+xhoi<br> <br />
<br />
Lane 10-11: pAAV-CMV-IFP (285), clone 1-2, bamhi+xhoi<br> <br />
<br />
[[Image:IMG_1275.JPG]] <br />
<br />
Lane 1-2: pAAV-CMV-IFP (285), clone 3-4, bamhi+xhoi <br> <br />
<br />
Lane 3-6: pAAV-rfp (347), clone 1-4, bamhi+xhoi<br> <br />
<br />
Lane 7-10: paav-egfp (348), clone 1-4, bamhi+xhoi<br> <br />
<br />
== '''Result intepretation/Summary''' ==<br />
<br />
Got all the positive colonies for above clones<br><br />
<br />
== '''Conclusion''' ==<br />
<br />
<br />
<br />
=== ===<br />
<br />
[[User:Haiyan]] 08:23, 18 Aug 2011 (EST)<br> <br />
<br />
[[Category:Cloning]]</div>
Hylei