Paul.xu: Created page with "NEB Competent Cells {| cellspacing="0" cellpadding="1" border="0" class="tableborder" |- | nowrap="nowrap" colspan="17" | Cloning Strain Properties |- | nowrap="nowrap" bgcolor..."

2012-02-25T17:01:11Z

Created page with "NEB Competent Cells {| cellspacing="0" cellpadding="1" border="0" class="tableborder" |- | nowrap="nowrap" colspan="17" | Cloning Strain Properties |- | nowrap="nowrap" bgcolor..."

New page

NEB Competent Cells

{| cellspacing="0" cellpadding="1" border="0" class="tableborder"
|-
| nowrap="nowrap" colspan="17" | Cloning Strain Properties
|-
| nowrap="nowrap" bgcolor="#c8d3a0" align="center" style="font-weight: bold;" | Strain Properties
| bgcolor="#c8d3a0" style="font-weight: bold;" | NEB#
| bgcolor="#c8d3a0" style="font-weight: bold;" | Transform 
Efficiency (cfu/µg)(1)

| bgcolor="#c8d3a0" style="font-weight: bold;" | Strain
| bgcolor="#c8d3a0" style="font-weight: bold;" | T1 
Phage Resistant

| bgcolor="#c8d3a0" style="font-weight: bold;" | Library 
Construc -tion(2)

| bgcolor="#c8d3a0" style="font-weight: bold;" | Cloning/ 
Sub cloning

| bgcolor="#c8d3a0" style="font-weight: bold;" | Blue/ 
White Screening

| bgcolor="#c8d3a0" | laclq
| bgcolor="#c8d3a0" | lysY
| bgcolor="#c8d3a0" style="font-weight: bold;" | Endo- 
nucleaseI Deficient(3)

| bgcolor="#c8d3a0" style="font-weight: bold;" | Protease(4) 
Deficient

| bgcolor="#c8d3a0" style="font-weight: bold;" | F´
| bgcolor="#c8d3a0" style="font-weight: bold;" | RecA¯
| bgcolor="#c8d3a0" style="font-weight: bold;" | T7 RNA 
Polyme -rase

| bgcolor="#c8d3a0" style="font-weight: bold;" | Cytoplasmic 
disulfide bond formation(5)

| bgcolor="#c8d3a0" style="font-weight: bold;" | Drug 
Resistance(6)

|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC2984.asp NEB Turbo]
| C2984
| 1-3x109
| K12
| √
| √
| √
| √
| √
| –
| √
| –
| √
| –
| –
| –
| nit
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC2987.asp NEB 5-alpha]
| bgcolor="#d4d4d4" | C2987
| bgcolor="#d4d4d4" | 1-3x109
| bgcolor="#d4d4d4" | K12
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | none
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC2992.asp NEB 5-alpha F'Iq]
| C2992
| 1-3x109
| K12
| √
| √
| √
| √
| √
| –
| √
| –
| √
| √
| –
| –
| tet
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC3019.asp NEB 10-beta]
| bgcolor="#d4d4d4" | C3019
| bgcolor="#d4d4d4" | 1-3x109
| bgcolor="#d4d4d4" | K12
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | str
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC2925.asp dam-/dcm-]
| C2925
| 1-3x106
| K12
| √
| √
| √
| –
| –
| –
| √
| –
| –
| –
| –
| –
| cam, str, nit
|-
| nowrap="nowrap" |  
|  
|  
|  
|  
|  
|  
|  
|  
|  
|  
|  
|  
|  
|  
|  
|  
|-
| nowrap="nowrap" colspan="17" | Protein Expression Strain Properties
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC2523.asp NEB Express]
| bgcolor="#d4d4d4" | C2523
| bgcolor="#d4d4d4" | 0.6-1x109
| bgcolor="#d4d4d4" | B
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | nit
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC3037.asp NEB Express Iq]
| C3037
| 0.6-1x109
| B
| √
| √
| –
| –
| √
| –
| √
| √
| –
| –
| –
| –
| cam, nit
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC2566.asp T7 Express]
| bgcolor="#d4d4d4" | C2566
| bgcolor="#d4d4d4" | 0.6-1x109
| bgcolor="#d4d4d4" | B
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | nit
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC3016.asp T7 Express Iq]
| C3016
| 0.6-1x109
| B
| √
| √
| –
| –
| √
| –
| √
| √
| –
| –
| √
| –
| cam, nit
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC3010.asp T7 Express lysY]
| bgcolor="#d4d4d4" | C3010
| bgcolor="#d4d4d4" | 0.6-1x109
| bgcolor="#d4d4d4" | B
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | cam, nit
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC3013.asp T7 Express lysY/Iq]
| C3013
| 0.6-1x109
| B
| √
| √
| –
| –
| √
| √
| √
| √
| –
| –
| √
| –
| cam, nit
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC3022.asp T7 Express Crystal]
| bgcolor="#d4d4d4" | C3022
| bgcolor="#d4d4d4" | 0.6-1x109
| bgcolor="#d4d4d4" | B
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | nit
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC3025.asp SHuffle]
| C3025
| 1 x 106
| K12
| √
| –
| –
| –
| √
| –
| –
| –
| √
| –
| –
| √
| str, spec
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC3026.asp SHuffle T7]
| bgcolor="#d4d4d4" | C3026
| bgcolor="#d4d4d4" | 1 x 106
| bgcolor="#d4d4d4" | K12
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | str, spec
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC3027.asp SHuffle T7 lysY]
| C3027
| 1 x 106
| K12
| √
| –
| –
| –
| √
| √
| –
| –
| –
| –
| √
| √
| cam, str, spec
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC3028.asp SHuffle Express]
| bgcolor="#d4d4d4" | C3028
| bgcolor="#d4d4d4" | 1 x 107
| bgcolor="#d4d4d4" | B
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | spec
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC3029.asp SHuffle T7 Express]
| C3029
| 1 x 107
| B
| √
| –
| –
| –
| √
| –
| √
| √
| –
| –
| √
| √
| spec(7)
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC3030.asp SHuffle T7 Express lysY]
| bgcolor="#d4d4d4" | C3030
| bgcolor="#d4d4d4" | 1 x 107
| bgcolor="#d4d4d4" | B
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | cam, 
spec(7)

|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC2530.asp BL21]
| C2530
| 1-5x107
| B
| √
| –
| –
| –
| –
| –
| –
| √
| –
| –
| –
| –
| –
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC2527.asp BL21(DE3)]
| bgcolor="#d4d4d4" | C2527
| bgcolor="#d4d4d4" | 1-5x107
| bgcolor="#d4d4d4" | B
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC2529.asp NiCo21(DE3)]
| C2529
| 1-5x107
| B
| √
| –
| –
| –
| –
| –
| –
| √
| –
| –
| √
| –
| –
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC2528.asp Lemo21(DE3)]
| bgcolor="#d4d4d4" | C2528
| bgcolor="#d4d4d4" | 1-3x107
| bgcolor="#d4d4d4" | B
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | √
| bgcolor="#d4d4d4" | –
| bgcolor="#d4d4d4" | cam
|}

(1) TE given are for high efficiency chemically competent strains. TE for electrocompetent strains are 1-4 x 1010cfu/mg. TE for subcloning strains are >1 x 106. 

(2) Restriction-deficient strain. (3) Important for plasmid preparation. (4) Lacks Lon and OmpT protease activity. (5) Constitutively expresses a chromosomal copy of the disulfide bond isomerase DsbC. (6) nit = nitrofurantoin, tet = tetracycline, cam = chloramphenicol, str = streptomycin, spec = spectinomycin 

(7) Resistance to low levels of streptomycin may be observed.

[[Category:Protein_Expression]] [[Category:Competent_Cells]]

NEB Competent Cells - Revision history

Paul.xu: Created page with "NEB Competent Cells {| cellspacing="0" cellpadding="1" border="0" class="tableborder" |- | nowrap="nowrap" colspan="17" | Cloning Strain Properties |- | nowrap="nowrap" bgcolor..."