Paul.xu at 02:47, 25 March 2012

2012-03-25T02:47:11Z

New page

Thermophilic DNA Polymerases 

{| width="100%" cellspacing="0" cellpadding="2" border="1" bgcolor="#e7dcc1" align="center"
|-
| height="30" align="center" |  
| height="30" | Vent
| height="30" align="center" | Vent (exo-)
| height="30" align="center" | Deep Vent
| height="30" align="center" | Deep Vent (exo-)
| height="30" align="center" | Taq
| height="30" align="center" | 9°Nm
| align="center" | Phusion**
| align="center" | LongAmp Taq
| align="center" | LongAmp Hot Start Taq
| align="center" | OneTaq
| align="center" | Phusion Hot Start Flex**
|-
| height="30" colspan="12" | Exonuclease Activities
|-
| 3'—>5' proofreading exonuclease
| bgcolor="#e5e5e5" align="center" | yes
| align="center" | no
| bgcolor="#e5e5e5" align="center" | yes
| align="center" | no
| bgcolor="#e5e5e5" align="center" | no
| align="center" | no*
| bgcolor="#e5e5e5" align="center" | yes
| align="center" | yes
| bgcolor="#e5e5e5" align="center" | yes
| align="center" | yes
| bgcolor="#e5e5e5" align="center" | yes
|-
| 5'—>3' exonuclease activity
| bgcolor="#e5e5e5" align="center" | no
| align="center" | no
| bgcolor="#e5e5e5" align="center" | no
| align="center" | no
| bgcolor="#e5e5e5" align="center" | yes
| align="center" | no
| bgcolor="#e5e5e5" align="center" | no
| align="center" | yes
| bgcolor="#e5e5e5" align="center" | yes
| align="center" | yes
| bgcolor="#e5e5e5" align="center" | no
|-
| height="30" colspan="12" | Thermal Stability
|-
| Half life at 95°C
| bgcolor="#e5e5e5" align="center" | 6.7 hours
| align="center" | 6.7 hours
| bgcolor="#e5e5e5" align="center" | 23 hours
| align="center" | 23 hours
| bgcolor="#e5e5e5" align="center" | 0.9 hours
| align="center" | 6.7 hours
| bgcolor="#e5e5e5" align="center" | >6 hours at 96°C
| align="center" | ND
| bgcolor="#e5e5e5" align="center" | ND
| align="center" | ND
| bgcolor="#e5e5e5" align="center" | ND
|-
| Half life at 100°C
| bgcolor="#e5e5e5" align="center" | 1.8 hours
| align="center" | 1.8 hours
| bgcolor="#e5e5e5" align="center" | 8 hours
| align="center" | 8 hours
| bgcolor="#e5e5e5" align="center" | <0.1 hours
| align="center" | 1.8 hours
| bgcolor="#e5e5e5" align="center" | 2 hours at 98°C
| align="center" | ND
| bgcolor="#e5e5e5" align="center" | ND
| align="center" | ND
| bgcolor="#e5e5e5" align="center" | ND
|-
| height="30" colspan="12" | Primer Extension Characteristics
|-
| Units/100 µl Primer Extension Rxn
| bgcolor="#e5e5e5" align="center" | 1-2 units
| align="center" | 2-4 units
| bgcolor="#e5e5e5" align="center" | 1-2 units
| align="center" | 2-4 units
| bgcolor="#e5e5e5" align="center" | 2-5 units
| align="center" | 1-2 units
| bgcolor="#e5e5e5" align="center" | 1-2 units
| align="center" | 10 units
| bgcolor="#e5e5e5" align="center" | 10 units
| align="center" | 2-5 units
| bgcolor="#e5e5e5" align="center" | 1-2 units
|-
| Error Rate (x10-6 base insertions)
| bgcolor="#e5e5e5" align="center" | 57
| align="center" | 190
| bgcolor="#e5e5e5" align="center" | ~20
| align="center" | ~190
| bgcolor="#e5e5e5" align="center" | 285
| align="center" | in progress
| bgcolor="#e5e5e5" align="center" | 0.44
| align="center" | ~140
| bgcolor="#e5e5e5" align="center" | ~140
| align="center" | ~140
| bgcolor="#e5e5e5" align="center" | 0.44
|- valign="top"
| Resulting DNA Ends
| valign="middle" bgcolor="#e5e5e5" align="center" | blunt ends
| valign="middle" align="center" | 3´ A
| valign="middle" bgcolor="#e5e5e5" align="center" | blunt ends
| valign="middle" align="center" | 3´ A
| valign="middle" bgcolor="#e5e5e5" align="center" | 3´ A
| valign="middle" align="center" | 3´ A blunt
| valign="middle" bgcolor="#e5e5e5" align="center" | blunt
| valign="middle" align="center" | 3´ A blunt
| valign="middle" bgcolor="#e5e5e5" align="center" | 3´ A blunt
| valign="middle" align="center" | 3´ A blunt
| valign="middle" bgcolor="#e5e5e5" align="center" | blunt ends
|- valign="top"
| Strand Displacement
| valign="middle" bgcolor="#e5e5e5" align="center" | yes; temp. dependent
| valign="middle" align="center" | yes; temp. dependent
| valign="middle" bgcolor="#e5e5e5" align="center" | yes; temp. dependent
| valign="middle" align="center" | yes; temp. dependent
| valign="middle" bgcolor="#e5e5e5" align="center" | no
| valign="middle" align="center" |
yes

| valign="middle" bgcolor="#e5e5e5" align="center" | no
| valign="middle" align="center" | yes; temp. dependent
| valign="middle" bgcolor="#e5e5e5" align="center" | yes; temp. dependent
| valign="middle" align="center" | yes; temp. dependent
| valign="middle" bgcolor="#e5e5e5" align="center" | no
|-
| Longest Primer Extension to Date
| bgcolor="#e5e5e5" align="center" | 13.2 kb
| align="center" | 15.0 kb
| bgcolor="#e5e5e5" align="center" | 14.0 kb
| align="center" | 15.0 kb
| bgcolor="#e5e5e5" align="center" | 8.1 kb
| align="center" | 14.0 kb
| bgcolor="#e5e5e5" align="center" | 37 kb
| align="center" | 40 kb
| bgcolor="#e5e5e5" align="center" | 40 kb
| align="center" | 15.0 kb
| bgcolor="#e5e5e5" align="center" | 20 kb
|}

*1-5% of wild type exonuclease activity is present in the modified enzyme Properties of DNA Polymerases

{| cellspacing="0" cellpadding="2" border="1" bgcolor="#e7dcc1" align="center"
|-
| nowrap="nowrap" align="center" |  
| align="center" | 5'—>3' Exonuclease
| align="center" | 3'—>5' Exonuclease
| align="center" | Error Rate(x10-6)
| align="center" | Strand Displacement
| align="center" | Nick Translation
| align="center" | Thermal Stability
| align="center" | Km dNTPs
| align="center" | Km DNA
| align="center" | Extend RNA Primer
| align="center" | Extension from Nick
| align="left" | Primary Applications
|-
| nowrap="nowrap" | 9°Nm DNA Polymerase
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" | +++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | ++++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 80 µM
| nowrap="nowrap" align="center" | 0.05 nM
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | Primer Extension
|-
| nowrap="nowrap" | Bst DNA Polymerase, Full Length
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" | _
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" |  
|-
| nowrap="nowrap" | Bst DNA Polymerase, Large Fragment
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" | ++++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | Strand Displacement Applications
|-
| nowrap="nowrap" | Bsu DNA Polymerase, Large fragment
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" | ++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" | Labeling, 2nd Strand Synthesis, Strand Displacement
|-
| nowrap="nowrap" | Crimson Taq DNA Polymerase
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" | _
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | ++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | PCR (routine)
|-
| nowrap="nowrap" | Phusion Hot Start Flex DNA Polymerase*
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | ++++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 0.44
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" | PCR (high fidelity, hot start)
|-
| nowrap="nowrap" | Deep Vent DNA Polymerase
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" | ++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | ++++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 50 µM
| nowrap="nowrap" align="center" | 0.01 nM
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | PCR (high fidelity)
|-
| nowrap="nowrap" | Deep Vent (exo–) DNA Polymerase
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" | +++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | ++++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | PCR (long)
|-
| nowrap="nowrap" | E. coli DNA Polymerase I
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | ++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 9
| nowrap="nowrap" align="center" | _
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 1-2 µM
| nowrap="nowrap" align="center" | 5 nM
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | Nick Translation
|-
| nowrap="nowrap" | Klenow Fragment 3´→ 5´ exo–
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 100
| nowrap="nowrap" align="center" | +++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | Labeling
|-
| nowrap="nowrap" | Klenow Fragment DNA Polymerase I
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | ++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 18
| nowrap="nowrap" align="center" | ++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 2 µM
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | Polishing Ends
|-
| nowrap="nowrap" | LongAmp Taq DNA Polymerase
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | ++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | ~140
| nowrap="nowrap" align="center" | _
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | ++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | PCR (routine, long)
|-
| nowrap="nowrap" | LongAmp Hot Start Taq DNA Polymerase
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | ++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | ~140
| nowrap="nowrap" align="center" | _
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | ++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | PCR (hot start, long)
|-
| nowrap="nowrap" | M-MuLV Reverse Transcriptase
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" | +++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 18 µM
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" | cDNA Synthesis
|-
| nowrap="nowrap" | OneTaq DNA Polymerase
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | ++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" | _
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | ++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | PCR (routine, difficult)
|-
| nowrap="nowrap" | OneTaq Hot Start DNA Polymerase
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | ++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" | _
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | ++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | PCR (hot start, routine, difficult)
|-
| nowrap="nowrap" | phi29 DNA Polymerase
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | ++++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" | +++++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 0.5 µM
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | Strand Displacement Applications
|-
| nowrap="nowrap" | Phusion High-Fidelity DNA Polymerase*
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | ++++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 0.44
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | PCR (high fidelity)
|-
| nowrap="nowrap" | Sulfolobus DNA Polymerase IV
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" | DNA Synthesis Across Template Lesions
|-
| nowrap="nowrap" | T4 DNA Polymerase
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | ++++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | <1
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 2 µM
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" | Polishing Ends, 2nd Strand Synthesis
|-
| nowrap="nowrap" | T7 DNA Polymerase
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | ++++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 15
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 18 µM
| nowrap="nowrap" align="center" | 18 nM
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" | Site Directed Mutagenesis
|-
| nowrap="nowrap" | Taq DNA Polymerase
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 285
| nowrap="nowrap" align="center" | _
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | ++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 13 µM
| nowrap="nowrap" align="center" | 2 nM
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | PCR (routine)
|-
| nowrap="nowrap" | Therminator DNA Polymerase
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | ++++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | Chain Terminator Applications
|-
| nowrap="nowrap" | Therminator II DNA Polymerase
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | ++++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | Incorporation of Modified Nucleotide Analogs
|-
| nowrap="nowrap" | Therminator III DNA Polymerase
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | ++++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | Incorporation of Modfied Nucleotides
|-
| nowrap="nowrap" | Therminator γ DNA Polymerase
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | ++++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" |  
| nowrap="nowrap" align="center" |  
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | +
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | Incorporation of gamma- phosphate Labeled deoxynucleotides
|-
| nowrap="nowrap" | Vent DNA Polymerase
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | ++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 57
| nowrap="nowrap" align="center" | ++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 60 µM
| nowrap="nowrap" align="center" | 0.1 nM
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | PCR (routine, high fidelity)
|-
| nowrap="nowrap" | Vent (exo–) DNA Polymerase
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | –
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 190
| nowrap="nowrap" align="center" | +++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +++
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | 40 µM
| nowrap="nowrap" align="center" | 0.1 nM
| nowrap="nowrap" bgcolor="#e5e5e5" align="center" | –
| nowrap="nowrap" align="center" | +
| nowrap="nowrap" bgcolor="#e5e5e5" | PCR, Sequencing
|}

{| width="100%" cellpadding="2" cellspacing="0" border="1" bgcolor="#e7dcc1"
| height="30" nowrap="nowrap" bgcolor="#e7dcc1" |  
| height="30" nowrap="nowrap" align="center" | 3'->5' Proofreading
| height="30" nowrap="nowrap" align="center" | Strand Displacement
| height="30" nowrap="nowrap" align="center" | Primary Applications
|- bgcolor="#e8e8e8"
| colspan="4" nowrap="nowrap" | Mesophilic DNA Polymerases
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0269.asp phi29 DNA Polymerase]
| align="center" | ++++
| align="center" | +++++
| Strand Displacement Applications
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0203.asp T4 DNA Polymerase]
| align="center" | +++++
| align="center" | -
| Polishing Ends, 2nd Strand Synthesis
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0209.asp DNA Polymerase I]
| align="center" | ++
| align="center" | -*
| Nick Translation
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0210.asp DNA Polymerase I, Klenow Fragment]
| align="center" | ++
| align="center" | ++
| Polishing Ends
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0212.asp Klenow Fragment (3' -> 5' exo-)]
| align="center" | -
| align="center" | +++
| Labeling
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0274.asp T7 DNA Polymerase (unmodified)]
| align="center" | ++++
| align="center" | -
| Site Directed Mutagenesis
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0252.asp Terminal Transferase]
| align="center" | -
| align="center" | NA
| 3' terminal Tailing
|- bgcolor="#e8e8e8"
| colspan="4" nowrap="nowrap" | Thermophilic DNA Polymerases
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productF-530.asp Phusion® High Fidelity DNA Polymerase]
| align="center" | +++++
| align="center" | -
| PCR (high fidelity)
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productF-540.asp Phusion® Hot Start High Fidelity DNA Polymerase]
| align="center" | +++++
| align="center" | -
| Hot Start PCR (high fidelity)
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productF-549.asp PhusionHot® Start II High Fidelity DNA Polymerase]
| align="center" | +++++
| align="center" | -
| Hot Start PCR (high fidelity)
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productF-120.asp Phire® Hot Start DNA Polymerase]
| align="center" | +
| align="center" | -
| Hot Start PCR (fast yield)
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productF-122.asp Phire® Hot Start II DNA Polymerase]
| align="center" | +
| align="center" | -
| Hot Start PCR (fast yield)
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productF-505.asp DyNAzyme™ EXT DNA Polymerase]
| align="center" | +
| align="center" | +
| PCR (difficult or long)
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productF-504.asp DyNAzyme™ II Hot Start DNA Polymerase]
| align="center" | -
| align="center" | -
| Hot Start PCR
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0267.asp Taq DNA Polymerase]
| align="center" | -
| align="center" | -*
| PCR (routine), Primer Extension
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0323.asp LongAmp Taq DNA Polymerase]
| align="center" | +
| align="center" | -*
| PCR (long, high-fidelity)
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0324.asp Crimson Taq DNA Polymerase]
| align="center" | -
| align="center" | -*
| PCR (routine)
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0254.asp VentR DNA Polymerase]
| align="center" | +++
| align="center" | ++
| PCR (high fidelity), Primer Extension
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0257.asp VentR (exo-) DNA Polymerase]
| align="center" | -
| align="center" | +++
| PCR, Sequencing
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0258.asp Deep VentR DNA Polymerase]
| align="center" | +++
| align="center" | ++
| PCR (high fidelity), Primer Extension
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0259.asp Deep VentR (exo-) DNA Polymerase]
| align="center" | -
| align="center" | +++
| PCR (long), Primer Extension
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0260.asp 9°Nm DNA Polymerase]
| align="center" | +
| align="center" | +++
| Primer Extension
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0261.asp Therminator™ DNA Polymerase]
| align="center" | -
| align="center" | +
| Chain Terminator Applications
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0266.asp Therminator™ II DNA Polymerase]
| align="center" | -
| align="center" | +
| Incorporation of modified substrates
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0275.asp Bst DNA Polymerase, Large Fragment]
| align="center" | -
| align="center" | +++
| Strand Displacement Applications
|- bgcolor="#e8e8e8"
| colspan="4" nowrap="nowrap" | Other Polymerases
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0253.asp M-MuLV Reverse Transcriptase]
| align="center" | -
| align="center" | +++
| cDNA Synthesis
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0277.asp AMV Reverse Transcriptase]
| align="center" | -
| align="center" | +++
| cDNA Synthesis
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0276.asp E. coli Poly(A) Polymerase]
| align="center" | -
| align="center" | NA
| 3´ labeling of RNA
|- valign="top"
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0255.asp phi6 RNA Polymerase]
| nowrap="nowrap" align="center" | -
| nowrap="nowrap" align="center" | NA
| nowrap="nowrap" | dsRNA Synthesis
|- valign="top"
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0251.asp T7 RNA Polymerase]
| nowrap="nowrap" align="center" | -
| nowrap="nowrap" align="center" | NA
| nowrap="nowrap" | High Yield RNA Production
|- valign="top"
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productM0207.asp SP6 Polymerase]
| nowrap="nowrap" align="center" | -
| nowrap="nowrap" align="center" | NA
| nowrap="nowrap" | RNA Production
|} 
* Degrades displaced strand.

[[Category:Knowledgebase]]

NEB DNA Polymerase - Revision history

Paul.xu at 02:47, 25 March 2012