https://eln.excellgen.com/index.php?action=history&feed=atom&title=Ni_Buffer_ANi Buffer A - Revision history2026-10-18T02:37:45ZRevision history for this page on the wikiMediaWiki 1.44.0https://eln.excellgen.com/index.php?title=Ni_Buffer_A&diff=19300&oldid=prevPaul.xu at 18:38, 23 September 20182018-09-23T18:38:47Z<p></p>
<p><b>New page</b></p><div>== 20 L Buffer A for Ni resin ==<br />
<br />
50 mM Tris-HCl, pH 8.0, 500 mM NaCl,&nbsp; 5 mM imidazole, 1 mM b-ME, 0.001&nbsp;mM EDTA<br />
<br />
2.5 L 4 M NaCl, or 584 g solid NaCl<br />
<br />
127 g Trizma HCL<br />
<br />
24g Trizma Base<br />
<br />
40 ul 0.5 M EDTA<br />
<br />
1.4 ml beta-ME<br />
<br />
6 g imidazole<br />
<br />
Add 4 L water to dissolve.<br />
<br />
Add 16 L ddH20 to make 20 L<br />
<br />
== 20 L Lysis Buffer for Ni resin ==<br />
<br />
50 mM Tris-HCl, pH8.0, 500 mM NaCl,&nbsp; 5 mM imidazole, 1 mM b-ME, 0.001 mM EDTA, 0.1% Triton X-100<br />
<br />
2.5 L 4 M NaCl, or 584 g solid NaCl<br />
<br />
127 g Trizma HCL<br />
<br />
24g Trizma Base<br />
<br />
40 ul 0.5 M EDTA<br />
<br />
1.4 ml beta-ME<br />
<br />
6 g imidazole<br/>20 g Triton X-100<br />
<br />
Add 4 L water to dissolve.<br />
<br />
Add 16 L ddH20 to make 20 L<br />
<br />
<br />
<br />
<br />
<br />
== Other Buffers ==<br />
<br />
1, 4 M NaCl, 4 L<br />
<br />
Dissolve 234 g NaCl in 4 L ddH2O<br />
<br />
2, Tris-HCl, 1 M, 1 L<br />
<br />
Dissolve 127 g Trizma HCL + 24g Trizma Base in 1 L ddH2O<br />
<br />
3, 0.5 M EDTA, 1 L<br />
<br />
dissolve 115 g in 1 L ddH2O<br />
<br />
<br />
[[Category:Buffer Recipe]]</div>Paul.xu