https://eln.excellgen.com/index.php?action=history&feed=atom&title=Ni_Buffer_BNi Buffer B - Revision history2026-10-18T07:26:30ZRevision history for this page on the wikiMediaWiki 1.44.0https://eln.excellgen.com/index.php?title=Ni_Buffer_B&diff=14815&oldid=prevExcellgen at 14:57, 7 June 20162016-06-07T14:57:26Z<p></p>
<p><b>New page</b></p><div>25 mM Tris-HCl, pH8.0, 100 mM NaCl,&nbsp; 150 mM imidazole, 1 mM b-ME<br />
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11.7 g solid NaCl<br />
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or 50 ml of 4 M NaCl<br />
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6.4 g Trizma HCl + 1.2g Trizma Base<br />
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or 50 ml of 1M Tris Buffer, pH 8.0<br />
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140 ul beta-ME<br />
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20 g imidazole<br />
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Add 1 L water to dissolve.<br />
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Alternatively, add 100 ml glycerol (final conc is 5%)<br />
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Add&nbsp; ddH20 to make 2 L<br />
[[Category:Buffer Recipe]]</div>Excellgen