https://eln.excellgen.com/index.php?action=history&feed=atom&title=Novice_protocols
Novice protocols - Revision history
2026-10-18T03:03:35Z
Revision history for this page on the wiki
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https://eln.excellgen.com/index.php?title=Novice_protocols&diff=21370&oldid=prev
Paul.xu at 22:32, 11 January 2021
2021-01-11T22:32:26Z
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<p><b>New page</b></p><div>{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| PCR from&nbsp;[[PCR_from_Plasmid_and_Genomic_DNA|Plasmid & Genomic DNA]]<br/><br />
| [[SubCloning|Subcloning]]<br/><br />
| [[Blunt-end_PCR_Cloning|Blunt-end Cloning&nbsp;]]<br/><br />
| [[Generic_Protein_Expression_Screening_Protocol|Protein Expression Screening]]<br/><br />
| [[Transformation|Transformation]]<br/><br />
|-<br />
| [[MiniPrep_using_Spin_Column|Miniprep]]<br/><br />
| [[Restriction_Digestion|Restriction Digestion]]<br/><br />
| Excellgen Generic&nbsp;[[Purification_of_His_Tag_Protein|Protein Purification]]&nbsp;Protocol<br/><br />
| [[His-Tat-NLS-Cre-Purification|Cre recombinase Purification]]<br/><br />
| [[Generic_Protein_Purification_Protocol|Generic Protein Purification Protocol]]<br/><br />
|-<br />
| Agarose Gel Electrophoresis<br/><br />
| SDS-PAGE<br/><br />
| [[Ni-Beads-Regeneration|Ni Resin Regeneration]]<br/><br />
| Shipping<br/><br />
| Print Label<br/><br />
|-<br />
| FPLC machine operation<br/><br />
| [[Cloning_of_a_PCR_Fragment_into_an_Expression_Vector|Direct cloning of a PCR product into an expression vector]]<br/><br />
| [[Sequencing|Sequencing]]<br/><br />
| [[PCR_Screening_and_Restriction_Digestion|Colony Screening by PCR]]<br/><br />
| [[Screnning_colony_by_restriction_digestion|Colony screening by restriction digestion]]<br/><br />
|-<br />
| [[DAN_Marker|DNA Ladder]]<br/><br />
| [[Cloning_after_PCR|Cloning after PCR]] (cohesive ends)<br/><br />
| [[T4_PNK|Phosphorylate oligo primer using T4 PNK]]<br/><br />
| [[2X_PCR_Mix|2X PCR Mix]]<br/><br />
| [[Culture_of_E_coli_for_protein_expression|Culture of E coli for protein expression]]<br/><br />
|-<br />
| Protein induction using IPTG<br/><br />
| Havest of E coli for protein expression<br/><br />
| E coli lysis, Homogenization and Centrifuge<br/><br />
| Load Cleared lysate to Ni column<br/><br />
| [[Ni_Buffer_A|Buffer A]]<br/><br />
|-<br />
| [[Purification_of_His-GST_Fusion_Protein|His-GST Fusion protein Purification]]<br />
| [[Sequencing_of_Difficult_template|Sequencing of Difficult Template]]<br/><br />
| [[Precipitation_of_DNA_using_Glyogen|Precipitate DNA using Glycogen]]<br />
| [[Vector_vs_Sequencing_Primers|Vector Sequencing Primers]]<br/><br />
| <br />
[[Purify_DNA_from_Gel_using_Isopropanol|Purify_DNA_from_gel_using_isopropanol]]<br />
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|-<br />
| [[Plasmid_DNA_purification_using_isopropanol_and_PEG|Plasmid DNA purification using isopropanol and PEG]]<br />
| [[Purification_of_VLP|VLP Purification]]<br />
| [[Gel_staining|gel_staining]], [[SDS-PAGE_Gel_Staining|2]]<br />
| [[Cleancap_mRNA_Invitro_Transcription#mRNA_for_transfection_in_vitro|mRNA for transfection in vitro]]<br />
| [[Cleancap_mRNA_Invitro_Transcription#mRNA_as_vaccine_for.C2.A0animal_study|mRNA as vaccine for animal]]<br />
|-<br />
| [http://18.206.161.119/eln/index.php/Promega_Protein_Expression Promega Protein Expression S30 T7]<br />
| [http://18.206.161.119/eln/index.php/Mini_prep_vacuum Mini Prep Using Vacuum]<br />
| [[Cloning_PCR_Product_by_Gibson_Mix|Gibson mix cloning]]<br />
| [[Lentivirus_Concentration_PEG|lentivirus concentration]]<br />
| <br/><br />
|-<br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|}<br />
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[[Tasks_for_This_Week|Tasks]] &nbsp;[[Special:NewPages|New Pages]]<br />
[[Category:My ELN]] [[Category:Tasks]]</div>
Paul.xu