https://eln.excellgen.com/index.php?action=history&feed=atom&title=Oligos_for_cloning_antibody Oligos for cloning antibody - Revision history 2026-10-19T10:09:31Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Oligos_for_cloning_antibody&diff=17843&oldid=prev Paul.xu at 20:21, 1 May 2018 2018-05-01T20:21:20Z <p></p> <p><b>New page</b></p><div>== Mouse B Cells or Hybridoma ==<br /> &lt;pre&gt;Primers VL-for d V(μl)<br /> original<br /> outer-for: ctacagcaggcccagccggccatggcggactacaaaG<br /> L Q Q A Q P A M A D Y K <br /> ctacagcaggcccagccggccatggcggacgcgcgcGa<br /> L Q Q A Q P A M A D A R <br /> MKYLLPTAAAGLLLLAAQPA <br /> ATGAAATACCTATTGCCTACGGCGGCCGCTGGATTGTTATTACTCGCGGCCCAGCCGGCCATGGCCgcgcgcGA<br /> M K Y L L P T A A A G L L L L A A Q P A M A A R <br /> <br /> original<br /> <br /> <br /> <br /> <br /> <br /> <br /> 19 TCAATAGTTGAACATAGCGGCCGCA GACATCCAGMTGACMCARTC<br /> VL-forκ1 catggcggactacaaaGACAWTGTTCTCACCCAGTC 2 6<br /> -- 3 TCAATAGTTGAACATAGCGGCCGCA SAAAWTGTKCTCACCCAGTC κ light chains F3<br /> VL-forκ2 catggcggactacaaaGACATCCAGATGACACAGWC 2 6<br /> <br /> VL-forκ3 catggcggactacaaaGATRTTGTGATGACCCAGWC 4 6<br /> --9 TCAATAGTTGAACATAGCGGCCGCA GATATCCAGATGACACAGAC κ light chains F9<br /> --13 TCAATAGTTGAACATAGCGGCCGCA GAYATTGTGATGACBCAGKC κ light chains F13<br /> VL-forκ4 catggcggactacaaaGACATTSTGMTGACCCAGTC 4 6<br /> --18 TCAATAGTTGAACATAGCGGCCGCA GACATTRTGATGWCACAGTC κ light chains F18<br /> --16 TCAATAGTTGAACATAGCGGCCGCA GAAAWTGTGYTGACCCAGTC κ light chains F16<br /> --15 TCAATAGTTGAACATAGCGGCCGCA GACATCYTGCTGACYCAGTC κ light chains F15<br /> --17 TCAATAGTTGAACATAGCGGCCGCA GAAACAACTGTGACCCAGTC κ light chains F17<br /> VL-forκ5 catggcggactacaaaGATGTTGTGVTGACCCAAAC 3 6<br /> --12 TCAATAGTTGAACATAGCGGCCGCA GATRTTKTGATGACYCARAC κ light chains F12<br /> GATGTTTTGATGACCCAAACTCCA 2002 Fab VκA<br /> VL-forκ6 catggcggactacaaaGACACAACTGTGACCCAGTC 1 3<br /> <br /> --19 TCAATAGTTGAACATAGCGGCCGCA GACATCCAGMTGACMCARTC κ light chains F19<br /> VL-forκ7 catggcggactacaaaGAYATTKTGCTCACTCAGTC 4 6<br /> -- 4 TCAATAGTTGAACATAGCGGCCGCA GAWATTGTGCTMACTCAGTC κ light chains F4<br /> VL-forκ8 catggcggactacaaaGATATTGTGATRACCCAGGM 4 6 <br /> note: VL-forκ8 was ordered from IDT as catggcggactacaaaGATATTGTGATRACCCAGGC<br /> maybe should be catggcggactacaaaGATATTGTGATRACCCAGGA<br /> -- 14 TCAATAGTTGAACATAGCGGCCGCA GATATTGTGATAACCCAGGA κ light chains F14<br /> VL-forκ8 catggcggactacaaaGATATTGTGATRACCCAGGC 4 6 modified<br /> <br /> VL-forκ9 catggcggactacaaaGACATTGTAATGACCCAATC 1 3<br /> -- 10 TCAATAGTTGAACATAGCGGCCGCA GACATTGTGCTGACCCAATC κ light chains F10<br /> -- 11 TCAATAGTTGAACATAGCGGCCGCA GACATYSTRATGACCCARTC κ light chains F11<br /> GACATTGTGCTCACCCAATCTCC 2002 Fab VκB<br /> VL-forκ10 catggcggactacaaaGACATTGTGATGWCACAGTC 2 6<br /> --6 TCAATAGTTGAACATAGCGGCCGCA GACATTGTGATGACMCAGTC κ light chains F6<br /> GACATTGTGCTRACCCAGTCTTCCA VκC Fab VκB <br /> -- 5 TCAATAGTTGAACATAGCGGCCGCA GACATTGTGCTRACACAGTC κ light chains F5<br /> VL-forκ11 catggcggactacaaaGATRTCCAGATGAMCCAGTC 4 6<br /> -- 7 TCAATAGTTGAACATAGCGGCCGCA GAYATCMAGATRAMCCAGTC κ light chains F7<br /> --8 TCAATAGTTGAACATAGCGGCCGCA GAYATCCAGATGAYTCAGTC κ light chains F8<br /> VL-forκ12 catggcggactacaaaGATGGAGAAACAACACAGGC 1 3<br /> <br /> optional<br /> VL-forλ1 catggcggactacaaaGACGCTGTTGTGACTCAGGA 1 1 <br /> VL-forλ2 catggcggactacaaaGACCYTGTGCTCACTCAGTC 2 2<br /> <br /> my design<br /> outer-for: TTATTACTCGCGGCCCAGCCGGCCATGGCCgcgcgcGA<br /> <br /> VL-forκ1 GGCCATGGCCgcgcgcGACAWTGTTCTCACCCAGTC 2 6<br /> VL-forκ2 GGCCATGGCCgcgcgcGACATCCAGATGACACAGWC 2 6<br /> VL-forκ3 GGCCATGGCCgcgcgcGATRTTGTGATGACCCAGWC 4 6<br /> VL-forκ4 GGCCATGGCCgcgcgcGACATTSTGMTGACCCAGTC 4 6<br /> VL-forκ5 GGCCATGGCCgcgcgcGATGTTGTGVTGACCCAAAC 3 6<br /> VL-forκ6 GGCCATGGCCgcgcgcGACACAACTGTGACCCAGTC 1 3<br /> VL-forκ7 GGCCATGGCCgcgcgcGAYATTKTGCTCACTCAGTC 4 6<br /> VL-forκ8 GGCCATGGCCgcgcgcGATATTGTGATRACCCAGGM 4 6<br /> VL-forκ9 GGCCATGGCCgcgcgcGACATTGTAATGACCCAATC 1 3<br /> VL-forκ10 GGCCATGGCCgcgcgcGACATTGTGATGWCACAGTC 2 6<br /> VL-forκ11 GGCCATGGCCgcgcgcGATRTCCAGATGAMCCAGTC 4 6<br /> VL-forκ12 GGCCATGGCCgcgcgcGATGGAGAAACAACACAGGC 1 3<br /> <br /> optional<br /> VL-forλ1 GGCCATGGCCgcgcgcGACGCTGTTGTGACTCAGGA 1 1<br /> VL-forλ2 catggcggacgcgcgcGACCYTGTGCTCACTCAGTC 2 2<br /> <br /> <br /> Primers VL-rev<br /> 5&#039; (Gly4Ser)3-linker | VL 3&#039;<br /> VL-revκ1 ggagccgccgccgcc (agaaccaccaccacc)2 GCGTTTBATTTCCAGCTTGG 3 25.3<br /> ggagccgccgccgccagaaccaccaccaccagaaccaccaccaccGCGTTTBATTTCCAGCTTGG<br /> <br /> (CCAAGCTGGAAATVAAACGC ggtggtggtggttct ggtggtggtggttct ggcggcggcggctcc)<br /> K L E I/L K R G G G G S G G G G S G G G G S <br /> <br /> VL-revκ2 ggagccgccgccgcc (agaaccaccaccacc)2 GCGTTTTATTTCCAATTTTG 1 12.7<br /> ggagccgccgccgccagaaccaccaccaccagaaccaccaccaccGCGTTTTATTTCCAATTTTG<br /> <br /> optional<br /> VL-revλ ggagccgccgccgcc (agaaccaccaccacc)2 GCCTAGGACAGTCAMCYTGG 4 2<br /> ggagccgccgccgccagaaccaccaccaccagaaccaccaccaccGCCTAGGACAGTCAMCYTGG <br /> <br /> <br /> Primers VH-for<br /> 5&#039; (Gly4Ser)2-linker BamHI | VH 3&#039;<br /> <br /> VH-for1 ggcggcggcggctccggtggtggt ggatcc GAGGTTCDSCTGCAACAGTY 12 4<br /> G G G G S G G G G S<br /> ggcggcggcggctccggtggtggt ggatcc GAGGTTCDSCTGCAACAGTC modified<br /> <br /> -- 30 TTCACGAGTCCAGCCTCAAGCAGT GAGRTCCAGCTGCAACARTC ---VH primer F30<br /> -- 33 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGMAGCTGGTGGAATC ---VH primer F33<br /> VH-for2 ggcggcggcggctccggtggtggt ggatcc CAGGTGCAAMTGMAGSAGTC 8 3<br /> VH-for3 ggcggcggcggctccggtggtggt ggatcc GAVGTGMWGCTGGTGGAGTC 12 4<br /> -- 35 TTCACGAGTCCAGCCTCAAGCAGT GARGTGCAGCTGKTGGAGWC ---VH primer F35<br /> VH-for4 ggcggcggcggctccggtggtggt ggatcc CAGGTTAYTCTGAAAGAGTC 2 2<br /> VH-for5 ggcggcggcggctccggtggtggt ggatcc GAKGTGCAGCTTCAGSAGTC 2 2<br /> --39 TTCACGAGTCCAGCCTCAAGCAGT GAAGTGAARMTTGAGGAGTC -----VH primer F39<br /> --34 TTCACGAGTCCAGCCTCAAGCAGT GAVGTGMWGCTSGTGGAGTC -----VH primer F34<br /> VH-for6 ggcggcggcggctccggtggtggt ggatcc CAGATCCAGTTSGYGCAGTC 4 2<br /> -- 43 TTCACGAGTCCAGCCTCAAGCAGT CAGATCCAGTTSGTRCAGTC -----VH primer F43<br /> ---31 TTCACGAGTCCAGCCTCAAGCAGT CAGGTYVAGCTGCAGCAGTC -----VH primer F31<br /> --Q_mVH_F6 CGTGACCACAGGCGTCCACAGC GACACAACTGTGACCCAGTC 2----<br /> <br /> VH-for7 ggcggcggcggctccggtggtggt ggatcc CAGRTCCAACTGCAGCAGYC 4 2<br /> VH-for8 ggcggcggcggctccggtggtggt ggatcc GAGGTGMAGCTASTTGAGWC 8 3<br /> --38 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGAAGCTTCTCRAGTC ---VH primer F38<br /> --37 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGCAGCTTGTTGAGTC ---VH primer F37<br /> --36 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGAAGCTGATGGAATC ---VH primer F36<br /> VH-for9 ggcggcggcggctccggtggtggt ggatcc GAAGTGAAGMTTGAGGAGTC 2 2<br /> --27 TTCACGAGTCCAGCCTCAAGCAGT GAKRTRCAGCTTMAGGAGTC ---VH primer F27<br /> VH-for10 ggcggcggcggctccggtggtggt ggatcc GATGTGAACCTGGAAGTGTC 1 1<br /> -- 42 TTCACGAGTCCAGCCTCAAGCAGT GATGTGAACCTGGAAGTGTC ---VH primer F42 <br /> VH-for11 ggcggcggcggctccggtggtggt ggatcc CAGATKCAGCTTMAGGAGTC 4 2<br /> VH-for12 ggcggcggcggctccggtggtggt ggatcc CAGGCTTATCTGCAGCAGTC 1 1<br /> <br /> -- 40 TTCACGAGTCCAGCCTCAAGCAGT CAGGTTACTCWGAAAGWGTCTG ---VH primer F40<br /> --29 TTCACGAGTCCAGCCTCAAGCAGT CAGGTGCAGMTGAAGSAGTC ---VH primer F29<br /> --28 TTCACGAGTCCAGCCTCAAGCAGT GAGKTYCAGCTBCAGCAGTC ---VH primer F28<br /> VH-for13 ggcggcggcggctccggtggtggt ggatcc CAGGTTCACCTACAACAGTC 1 1<br /> --41 TTCACGAGTCCAGCCTCAAGCAGT CAGGTCCAACTGCAGCAGCC ---VH primer F41<br /> --32 TTCACGAGTCCAGCCTCAAGCAGT CAGGTYCARCTGCAGCAGTC ---VH primer F32<br /> VH-for14 ggcggcggcggctccggtggtggt ggatcc CAGGTGCAGCTTGTAGAGAC 1 1<br /> --44 TTCACGAGTCCAGCCTCAAGCAGT GAGGTRCAGCTKGTAGAGAC ---VH primer F44<br /> VH-for15 ggcggcggcggctccggtggtggt ggatcc GARGTGMAGCTGKTGGAGAC 8 3<br /> <br /> <br /> <br /> Primers VH-rev<br /> 5&#039; 3&#039;<br /> outer-rev cggagtcaggcccccgag<br /> 5&#039; SfiI VH 3&#039;<br /> VH-rev1 cggagtcaggcccccgaggc CGAGGAGACGGTGACMGTGG 2 2<br /> VH-rev2 cggagtcaggcccccgaggc CGCAGAGACAGTGACCAGAG 1 1<br /> ____.SfiI____<br /> (CTCTGGTCACTGTCTCTGCGgcctcgggggcctgactccg)<br /> L V T V S A A S G A - <br /> <br /> <br /> VH-rev3 cggagtcaggcccccgaggc CGAGGAGACTGTGAGASTGG 2 2<br /> <br /> <br /> <br /> ==========<br /> 2002 Antibody Display Methods and Protocols,<br /> Fab, Mouse<br /> 3&#039;primer<br /> LC Cκ1 CATG TCTAGA ACACTCATTCCTGTTGAAGCTCTTG<br /> <br /> 5&#039; primers<br /> LC <br /> VκA CATG GAGCTC GATGTTTTGATGACCCAAACTCCA<br /> VκB GATC GAGCTC GACATTGTGCTCACCCAATCTCC<br /> VκC CATG GAGCTC GACATTGTGCTRACCCAGTCTTCCA<br /> VκD CATG GAGCTC GACATCCAGATGACNCAGTCTCAA<br /> VκE CATG GAGCTC CAAATTGTTCTCACCCAGTCTCCA<br /> VκF CATG GAGCTC GAAAATGTGCTTCACCCAGTCTCCA<br /> <br /> 3&#039; primers<br /> HC <br /> Igγ1 AGGCTT ACTAGT TATGCAAGGCTTACAACC<br /> Igγ2A AGGCTT ACTAGT ACAGGGCTTGATTGTGGGCCC<br /> Igγ2B AGGCTT ACTAGT ACAGGGGTTCAGTGTTGAAATGG<br /> 5&#039; primers<br /> <br /> HC IA TGGAGGCTT CTCGAG GAKGTGCAGCTTCAGGAGTC<br /> IB TGGAGGCTT CTCGAG CAGGTGCAGCTGAAGSAGTC<br /> IIA TGGAGGCTT CTCGAG SAGGTCCAGCTGCARCAGTC<br /> IIB TGGAGGCTT CTCGAG CAGGTCCARCTGCAGCAGYTTGG<br /> IIC TGG AGGCTT CTCGAG GAGGTTCAGCTGCAGCAGTC<br /> IIIA TGGAGGCTT CTCGAG GARGTGAAGCTGGTGGARTCTGG<br /> IIIB TGGAGGCTT CTCGAG GAGGTGAAGCTTCTGGAGTCTGG<br /> IIIC TGGAGGCTT CTCGAG GAAGTGAAGCTTGAGGAGWCTGG<br /> IIIDA TGGAGGCTT CTCGAG GAAGTGCAGCTGGTGGAGTCTGG<br /> IIIDB TGGAGGCTT CTCGAG GAAGTGATGCTGGTGGAGTCTGG<br /> VA TGGAGGCTT CTCGAG GAGGTYCAGCTKCAGCAG<br /> CH1 GCCAAAACGACACCCCCA<br /> <br /> <br /> ====<br /> leader sequence for the κ light chains<br /> GCCACCATGGAGTTTCAGACCCAGGTACTCATGTCCCTGCTGCTCTGCATGTCTGGTGC<br /> M E F Q T Q V L M S L L L C M S G <br /> GGCCGCACCAGACATGCAGAGCAGCAGGGACATGAGTACCTGGGTCTGAAACTCCATGGTGGC<br /> <br /> κ light chains F<br /> 3 TCAATAGTTGAACATAGCGGCCGCA SAAAWTGTKCTCACCCAGTC<br /> 4 TCAATAGTTGAACATAGCGGCCGCA GAWATTGTGCTMACTCAGTC<br /> 5 TCAATAGTTGAACATAGCGGCCGCA GACATTGTGCTRACACAGTC<br /> 6 TCAATAGTTGAACATAGCGGCCGCA GACATTGTGATGACMCAGTC<br /> 7 TCAATAGTTGAACATAGCGGCCGCA GAYATCMAGATRAMCCAGTC<br /> 8 TCAATAGTTGAACATAGCGGCCGCA GAYATCCAGATGAYTCAGTC<br /> 9 TCAATAGTTGAACATAGCGGCCGCA GATATCCAGATGACACAGAC<br /> 10 TCAATAGTTGAACATAGCGGCCGCA GACATTGTGCTGACCCAATC<br /> 11 TCAATAGTTGAACATAGCGGCCGCA GACATYSTRATGACCCARTC<br /> 12 TCAATAGTTGAACATAGCGGCCGCA GATRTTKTGATGACYCARAC<br /> 13 TCAATAGTTGAACATAGCGGCCGCA GAYATTGTGATGACBCAGKC<br /> 14 TCAATAGTTGAACATAGCGGCCGCA GATATTGTGATAACCCAGGA<br /> 15 TCAATAGTTGAACATAGCGGCCGCA GACATCYTGCTGACYCAGTC<br /> 16 TCAATAGTTGAACATAGCGGCCGCA GAAAWTGTGYTGACCCAGTC<br /> 17 TCAATAGTTGAACATAGCGGCCGCA GAAACAACTGTGACCCAGTC<br /> 18 TCAATAGTTGAACATAGCGGCCGCA GACATTRTGATGWCACAGTC<br /> 19 TCAATAGTTGAACATAGCGGCCGCA GACATCCAGMTGACMCARTC<br /> <br /> κ light chains R<br /> 20 GGATACAGTTGGTGCAGCATCAGCCC<br /> <br /> Primers used for the generation of the joining fragment<br /> 21 GGGCTGATGCTGCACCAACTGTA<br /> 22 TCAAGTGCAAAGACTCACTTTATTG<br /> 23 CAATAAAGTGAGTCTTTGCACTTGATAGTTATTAATAGTAATCAATTACG<br /> 24 TAGCAGAACAGGCAGCTTCATGGTGGCCTGGGAGTGGACACCTGTGGAGAG<br /> 25 ATGAAGCTGCCTGTTCTGCTAGTG<br /> 26 ACTGCTTGAGGCTGGACTCGTGAACAATAGCAGC<br /> <br /> Degenerate forward primers used for the amplification of rearranged<br /> heavy chains<br /> 27 TTCACGAGTCCAGCCTCAAGCAGT GAKRTRCAGCTTMAGGAGTC<br /> 28 TTCACGAGTCCAGCCTCAAGCAGT GAGKTYCAGCTBCAGCAGTC<br /> 29 TTCACGAGTCCAGCCTCAAGCAGT CAGGTGCAGMTGAAGSAGTC<br /> 30 TTCACGAGTCCAGCCTCAAGCAGT GAGRTCCAGCTGCAACARTC<br /> 31 TTCACGAGTCCAGCCTCAAGCAGT CAGGTYVAGCTGCAGCAGTC<br /> 32 TTCACGAGTCCAGCCTCAAGCAGT CAGGTYCARCTGCAGCAGTC<br /> 33 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGMAGCTGGTGGAATC<br /> 34 TTCACGAGTCCAGCCTCAAGCAGT GAVGTGMWGCTSGTGGAGTC<br /> 35 TTCACGAGTCCAGCCTCAAGCAGT GARGTGCAGCTGKTGGAGWC<br /> 36 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGAAGCTGATGGAATC<br /> 37 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGCAGCTTGTTGAGTC<br /> 38 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGAAGCTTCTCRAGTC<br /> 39 TTCACGAGTCCAGCCTCAAGCAGT GAAGTGAARMTTGAGGAGTC<br /> 40 TTCACGAGTCCAGCCTCAAGCAGT CAGGTTACTCWGAAAGWGTCTG<br /> 41 TTCACGAGTCCAGCCTCAAGCAGT CAGGTCCAACTGCAGCAGCC<br /> 42 TTCACGAGTCCAGCCTCAAGCAGT GATGTGAACCTGGAAGTGTC<br /> 43 TTCACGAGTCCAGCCTCAAGCAGT CAGATCCAGTTSGTRCAGTC<br /> 44 TTCACGAGTCCAGCCTCAAGCAGT GAGGTRCAGCTKGTAGAGAC<br /> <br /> Reverse primers used for the amplification of rearranged heavy chains<br /> 45 CTATTCTAGCTAATCTAGGCGCGCCGAGGAGACGGTGACCGTGGTCC<br /> (GGACCACGGTCACCGTCTCCTCGGCGCGCCTAGATTAGCTAGAATAG)<br /> T T V T V S S A R L D - <br /> 46 CTATTCTAGCTAATCTAGGCGCGCCGAGGAGACTGTGAGAGTGGTGC<br /> (GCACCACTCTCACAGTCTCCTCGGCGCGCCTAGATTAGCTAGAATAG)<br /> T T L T V S S A R L D - <br /> <br /> 47 CTATTCTAGCTAATCTAGGCGCGCCGCAGAGACAGTGACCAGAGTCC<br /> (GGACTCTGGTCACTGTCTCTGCGGCGCGCCTAGATTAGCTAGAATAG)<br /> T L V T V S A A R L D - <br /> <br /> 48 CTATTCTAGCTAATCTAGGCGCGCCGAGGAGACGGTGACTGAGGTTC<br /> (GAACCTCAGTCACCGTCTCCTCGGCGCGCCTAGATTAGCTAGAATAG)<br /> T S V T V S S A R L D - <br /> <br /> ======<br /> Evaluation of Antigen-Conjugated Fluorescent Beads to Identify Antigen-Specific B Cells<br /> <br /> <br /> VH1L CCATGGACTGGACCTGGA Heavy chain<br /> VH2L CAGATGGACATACTTTGTTCCAC Heavy chain<br /> VH3L CCATGGAGTTTGGGCTGAGC Heavy chain<br /> VH4L CGATGAAACACCTGTGGTTCTT Heavy chain<br /> VH5L ATGGGGTCAACCGCCATCCT Heavy chain<br /> VH6L GATGTCTGTCTCCTTCCTCAT Heavy chain<br /> VH1F CAGGTGCAGCTGGTGCAGTCTG Heavy chain<br /> VH2F GTCTTGTCCCAGGTCAACTTAAGGGAGTCTT Heavy chain<br /> VH3F GAGGTGCAGCTGGTGGAGTCTG Heavy chain<br /> VH4F CAGGTGCAGCTGCAGGAGTCGG Heavy chain<br /> VH5F GAGGTGCAGCTGCTGCAGTCTG Heavy chain<br /> VH6F CTGTCACAGGTACAGCTGCAGCAGTCAG Heavy chain<br /> IGREV CCAACTCTCTTGTCCACCTTGG Heavy chain<br /> L-Vk1/2 ATGAGGGTCCCCGCTCAGCTGCTGG Kappa light chain<br /> L-Vk3 CTCTTCCTCCTGCTACTCTGGCTCCCAG Kappa light chain<br /> L-Vk4 ATTTCTCTGTTGCTCTGGATCTCTG Kappa light chain<br /> Ck 543-566 GTTTCTCGTAGTCTGCTTTGCTCA Kappa light chain<br /> Pan-Vk ATGACCCAGACTCCATCCACCCTG Kappa light chain<br /> Ck 494-516 GTGCTGTCCTTGCTGTCCTGCT Kappa light chain<br /> L-Vλ1 GGTCCTGGGCCCAGTCTGTGCTG Lambda light chain<br /> L-Vλ12 GGTCCTGGGCCCAGTCTGCCCTG Lambda light chain<br /> L-Vλ3 GCTCTGTGACCTCCTATGAGCTG Lambda light chain<br /> L-Vλ4/5 GGTCTCTCTCGCAGCCTGTGCTG Lambda light chain<br /> L-Vλ6 GTTCTTGGGCCAATTTTATGCTG Lambda light chain<br /> L-Vλ7 GGTCCAATTCCCAGGCTGTGGTG Lambda light chain<br /> L-Vλ8 GAGTGGATTCTCAGACTGTGGTG Lambda light chain<br /> Cλ-1 CACCAGTGTGGCCTTGTTGGCTTG Lambda light chain<br /> F-Vλ1 CAGTCTGTGTTGACGCAGCC Lambda light chain<br /> F-Vλ2 CAGTCTGCCCTGACTCAGCC Lambda light chain<br /> F-Vλa3 TCTTATGAGCTGACACAGCCA Lambda light chain<br /> F-Vλa3l TCTTCTGAGCTGACTCAGGACCC Lambda light chain<br /> F-Vλ4ab GAGCTTGTGCTGACTCAATC Lambda light chain<br /> F-Vλ4c CTGCCTGTGCTGACTCAGC Lambda light chain<br /> F-Vλ5/9 CAGCCTGTGCTGACTCAGCC Lambda light chain<br /> F-Vλa6 AATTTTATGCTGACTCAGCCCCACT Lambda light chain<br /> F-Vλ7/8 CAGACTGTGGTGACCCAGGAG Lambda light chain<br /> F-Vλa10 CAGGCAGGGCTGACTCAG Lambda light chain<br /> Cλ-2 GGGTGGGAACAGAGTGACC Lambda light chain<br /> ======<br /> HybriFree: a robust and rapid method for<br /> the development of monoclonal antibodies<br /> from different host species<br /> <br /> 5&#039;-3&#039; sequences of mouse primers designed by ref. [17] <br /> <br /> VH forward primer mix proportion in VHF mix<br /> Q_mVH_F1 CGTGACCACAGGCGTCCACAGC GAGGTTCDSCTGCAACAGTY 4<br /> Q_mVH_F2 CGTGACCACAGGCGTCCACAGC CAGGTGCAAMTGMAGSAGTC 3<br /> Q_mVH_F3 CGTGACCACAGGCGTCCACAGC GAVGTGMWGCTGGTGGAGTC 4<br /> Q_mVH_F4 CGTGACCACAGGCGTCCACAGC CAGGTTAYTCTGAAAGAGTC 2<br /> Q_mVH_F5 CGTGACCACAGGCGTCCACAGC GAKGTGCAGCTTCAGSAGTC 2<br /> Q_mVH_F6 CGTGACCACAGGCGTCCACAGC GACACAACTGTGACCCAGTC 2<br /> Q_mVH_F7 CGTGACCACAGGCGTCCACAGC CAGRTCCAACTGCAGCAGYC 2<br /> Q_mVH_F8 CGTGACCACAGGCGTCCACAGC GAGGTGMAGCTASTTGAGWC 3<br /> Q_mVH_F9 CGTGACCACAGGCGTCCACAGC GAAGTGAAGMTTGAGGAGTC 2<br /> Q_mVH_F10 CGTGACCACAGGCGTCCACAGC GATGTGAACCTGGAAGTGTC 1<br /> Q_mVH_F11 CGTGACCACAGGCGTCCACAGC CAGATKCAGCTTMAGGAGTC 2<br /> Q_mVH_F12 CGTGACCACAGGCGTCCACAGC CAGGCTTATCTGCAGCAGTC 1<br /> Q_mVH_F13 CGTGACCACAGGCGTCCACAGC CAGGTTCACCTACAACAGTC 1<br /> Q_mVH_F14 CGTGACCACAGGCGTCCACAGC CAGGTGCAGCTTGTAGAGAC 1<br /> Q_mVH_F15 CGTGACCACAGGCGTCCACAGC GARGTGMAGCTGKTGGAGAC 3<br /> <br /> VH reverse primer mix proportion in VHR mix<br /> Q_mVH_R1 CACCACCACTACCGCCTCCTCCTGAGGAGACGGTGACMGTGG 2<br /> Q_mVH_R2 CACCACCACTACCGCCTCCTCCTGCAGAGACAGTGACCAGAG 1<br /> Q_mVH_R3 CACCACCACTACCGCCTCCTCCTGAGGAGACTGTGAGASTGG 2<br /> <br /> VLκ forward primer mix proportion in VLκF mix<br /> Q_mVLκ_F1 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGACAWTGTTCTCACCCAGTC 2<br /> Q_mVLκ_F2 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGACATCCAGATGACACAGWC 2<br /> Q_mVLκ_F3 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGATRTTGTGATGACCCAGWC 2<br /> Q_mVLκ_F4 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGACATTSTGMTGACCCAGTC 2<br /> Q_mVLκ_F5 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGATGTTGTGVTGACCCAAAC 2<br /> Q_mVLκ_F6 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGACACAACTGTGACCCAGTC 1<br /> Q_mVLκ_F7 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGAYATTKTGCTCACTCAGTC 2<br /> Q_mVLκ_F8 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGATATTGTGATRACCCAGGM 2<br /> Q_mVLκ_F9 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGACATTGTAATGACCCAATC 1<br /> Q_mVLκ_F10 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGACATTGTGATGWCACAGTC 2<br /> Q_mVLκ_F11 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGATRTCCAGATGAMCCAGTC 2<br /> Q_mVLκ_F12 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGATGGAGAAACAACACAGGC 1<br /> <br /> VLκ reverse primer mix proportion in VLκR mix<br /> Q_mVLκ_R1x GGACAGGTGTGGGTCTTGTCAGCCCGTTTBATTTCCAGCTTGG 2<br /> (CCAAGCTGGAAATVAAACGGGCTGACAAGACCCACACCTGTCC)<br /> K L E X K R A D K T H T C <br /> Q_mVLκ_R2x GGACAGGTGTGGGTCTTGTCAGCCCGTTTTATTTCCAATTTTG 1<br /> (CAAAATTGGAAATAAAACGGGCTGACAAGACCCACACCTGTCC)<br /> K L E I K R A D K T H T C <br /> &lt;/pre&gt;<br /> &amp;#x5B;&amp;#x5B;Category:Antibodies&amp;#x5D;&amp;#x5D;</div> Paul.xu