https://eln.excellgen.com/index.php?action=history&feed=atom&title=PCMV5-Fc-%283c%29-NPR1-Cyto-WPRE-oriP PCMV5-Fc-(3c)-NPR1-Cyto-WPRE-oriP - Revision history 2026-10-18T12:55:32Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=PCMV5-Fc-(3c)-NPR1-Cyto-WPRE-oriP&diff=6112&oldid=prev Xiuli.huang at 17:59, 20 September 2012 2012-09-20T17:59:38Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 1377pCMV5-Fc-(3c)-NPR1-Cyto-WPRE-oriP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; subcloning of 3C into pDB.His.MBP(V14) <br /> <br /> &amp;lt;br&amp;gt; &#039;&#039;&#039;Date:&amp;amp;nbsp;&#039;&#039;&#039; 2012-09-18&amp;lt;br&amp;gt; <br /> <br /> &amp;lt;br&amp;gt; &#039;&#039;&#039;Notebook:&amp;amp;amp;nbsp;&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID: 1375&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters: Xiuli&#039;&#039;&#039; <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> Cloning <br /> <br /> [[Image:PDB.HIS 3C.jpg]] <br /> <br /> 09-19-2012 A.Restriction enzyme digestion: Vector V14 pDB.His.MBP and 1197 pDB.His.GST.3C with NdeI and XhoI, B.Gel purification (elute with 15 ul H2O), and ligation with T7 ligase at RT for2 hs (total volume in 30ul) C Transformation: DH5a + 20 ul ligation Re.&lt;br&gt;&lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Xiuli.huang