https://eln.excellgen.com/index.php?action=history&feed=atom&title=PCR_Enhancer_MixPCR Enhancer Mix - Revision history2026-10-18T10:14:01ZRevision history for this page on the wikiMediaWiki 1.44.0https://eln.excellgen.com/index.php?title=PCR_Enhancer_Mix&diff=19633&oldid=prevPaul.xu at 20:53, 1 December 20182018-12-01T20:53:39Z<p></p>
<p><b>New page</b></p><div>== 1, 5X Enhancer: ==<br />
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4 M betaine, 10 mM DTT, and 10% DMSO,&nbsp;55 ug/ml BSA&nbsp;<br />
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15 ml: 7 g&nbsp;betaine, 150 ul 1 M DTT, 1.5 ml DMSO, 83 ul of 10mg/ml BSA<br />
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stable at 20 C for at least 3 months<br />
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Taq reaction buffer containing final concentrations of 65 mM TrisHCl, 16.6 mM (NH4)2SO4, 3.1 mM MgCl2, and 0.01% (v/v) Tween 20 at a pH of 8.8&nbsp;<br />
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== 2, Real-time PCR enhancer ==<br />
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PCR mix supplemented with 1 M 1,2-propanediol and 0.2 M trehalose (PT enhancer).<br />
[[Category:Buffer Recipe]]</div>Paul.xu