PCR Stabilizer - Revision history

Paul.xu: Created page with "https://www.google.com/patents/US5861251
2018-02-07T04:42:46Z

Created page with "<span style="color: rgb(34, 34, 34); font-family: Arial, sans-serif; font-size: 13.3333px;"></span>https://www.google.com/patents/US5861251 <span style="color: rgb(34, 34, 34..."

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<span style="color: rgb(34, 34, 34); font-family: Arial, sans-serif; font-size: 13.3333px;"></span>https://www.google.com/patents/US5861251

<span style="color: rgb(34, 34, 34); font-family: Arial, sans-serif; font-size: 13.3333px;">Performance of PCR is not affected by the addition of stabilizers such as ammonium sulfate, glycerol, glucitol, PEG-8000 or Thesit, provided that both ammonium sulfate and glycerol are not added to the mixture simultaneously.</span>

<span style="color: rgb(34, 34, 34); font-family: Arial, sans-serif; font-size: 13.3333px;">lyophilization does not have an effect on PCR, provided that ammonium sulfate is not added to the PCR mixture</span>

<span style="color: rgb(34, 34, 34); font-family: Arial, sans-serif; font-size: 13.3333px;">the addition of stabilizer (such as 4% glycerol, 20 mM glucitol, 0.1% PEG-8000 and 0.1% Thesit)  improves heat stability of PCR; and, the lyophilized PCR mixture containing 20 mM glucitol provides a highly improved heat stability.</span>

<span style="color: rgb(34, 34, 34); font-family: Arial, sans-serif; font-size: 13.3333px;">the addition of stabilizer (100 mM glucitol) and lyophilization resulted in heat stability of a PCR mixture Glucitol which is a polyol, conferred the most improved heat stability on the lyophilized PCR mixture.</span>

<span style="color: rgb(34, 34, 34); font-family: Arial, sans-serif; font-size: 13.3333px;">the lyophilized reaction mixtures containing 20 mM of glucose, mannitol, galacitol or glucitol as a stabilizer,  improved heat stability of the lyophilized PCR mixture</span>

<span style="color: rgb(34, 34, 34); font-family: Arial, sans-serif; font-size: 13.3333px;">bromophenol blue, xylene cyanole, bromocresol red, and cresol red ca be added to  in the preparation of the lyophilized PCR mixtures., but not methyl green, </span>
[[Category:QPCR RT-PCR]]