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PDB-His-NusA-StrepII - Revision history
2026-10-18T13:20:58Z
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Paul.xu at 22:25, 20 April 2011
2011-04-20T22:25:52Z
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<p><b>New page</b></p><div>Primer-F <br />
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StrepII-T: agcttCTGCAGctcgagTGGAGCCACCCGCAGTTCGAAAAGTgAa <br />
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Primer-R <br />
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StrepII-B: tcgatTcACTTTTCGAACTGCGGGTGGCTCCActcgagCTGCAGa <br />
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anneal these two oligos and ligate to HindIII and Xho1 digested pDB.His.NusA. Transform Top 10 competent cells<br> <br />
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[[Image:PDB-His-NusA StrepII digested with Pst1 Xba1.JPG]]<br> <br />
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mini-prep digest with Xba1+Pst1<br> <br />
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<br> --[[User:Paul.xu|Paul.xu]] 17:24, 20 April 2011 (EST)<br> <br />
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[[Category:Vectors]]</div>
Paul.xu