https://eln.excellgen.com/index.php?action=history&feed=atom&title=PDB_HIS_GST_Strep_II 2026-10-18T04:03:14Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=PDB_HIS_GST_Strep_II&diff=814&oldid=prev 2011-05-06T23:24:41Z

<p>moved <a href="/index.php?title=PDB_HIS_GST_Strep_II_cloning" class="mw-redirect" title="PDB HIS GST Strep II cloning">PDB HIS GST Strep II cloning</a> to <a href="/index.php?title=PDB_HIS_GST_Strep_II" title="PDB HIS GST Strep II">PDB HIS GST Strep II</a></p> <p><b>New page</b></p><div>Clone # 200&lt;br&gt; <br /> <br /> Digest pDB.His.GST with &lt;br&gt; <br /> <br /> Add StrepII tag to C-terminus &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> HindIII PstI Xho1 StrepII tag broken Xho1 &lt;br&gt; <br /> <br /> 5&#039; agctt CTG CAG ctc gag TGG AGC CAC CCG CAG TTC GAA AAG TgA a 3&#039; &lt;br&gt; <br /> <br /> 3&#039; a GAC GTC gag ctc ACC TCG GTG GGC GTC AAG CTT TTC AcT tag ct 5&#039; &lt;br&gt; <br /> <br /> StrepII-T: agcttCTGCAGctcgagTGGAGCCACCCGCAGTTCGAAAAGTgAa &lt;br&gt; <br /> <br /> StrepII-B: tcgatTcACTTTTCGAACTGCGGGTGGCTCCActcgagCTGCAGa&lt;br&gt; <br /> <br /> anneal these two oligos and ligate to HindIII and Xho1 digested pDB.His.GST&lt;br&gt; <br /> <br /> [[Image:PDBHisGST.StrepoII digestion Xba1 Pst1.JPG]]&lt;br&gt; <br /> <br /> digestion with Xba1+ Pst1&lt;br&gt; --[[User:Paul.xu|Paul.xu]] 18:13, 6 May 2011 (EST) <br /> <br /> [[Category:Cloning]]</div>

Paul.xu