https://eln.excellgen.com/index.php?action=history&feed=atom&title=PET28-TurboTEV PET28-TurboTEV - Revision history 2026-10-18T16:58:04Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=PET28-TurboTEV&diff=1131&oldid=prev Hylei at 21:05, 22 June 2011 2011-06-22T21:05:22Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Genesynthesis of TEV to pET28b&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Genesynthesis of TEV to pET28b. <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2011-6-22 <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; Haiyan-1<br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039;002943<br /> <br /> &#039;&#039;&#039;Clone ID:&#039;&#039;&#039; 203.1<br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Paul, Haiyan<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Subcloning ===<br /> <br /> Date: 2011-6-22 <br /> <br /> PCR amplified TEV1-20 and ligate to the EcoRI-SalI digested pET28b &lt;br&gt; <br /> <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2011-6-22&lt;br&gt; <br /> Use T7 F,R to PCR screening pET28b-TEV (clone #1-10)<br /> <br /> &lt;br&gt;<br /> <br /> [[Image:PET28b-TEV-pcr-06222011.JPG‎ ]] <br /> <br /> Lane 1-10, 10 colonies pcr amplify <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> lane 2 and 5 should be good, inoculate to do the minipre <br /> *Sequencing Result:&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> Restriction digestion &lt;br&gt; <br /> <br /> shows&amp;nbsp; pLentiGP clone #1 may be good, &lt;br&gt; <br /> <br /> pLVGP-PKAalpha 3+4 is good&lt;br&gt; <br /> <br /> === ===<br /> <br /> [[User:Paul.xu|Paul.xu]] 08:23, 15 June 2011 (EST)&lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Hylei