https://eln.excellgen.com/index.php?action=history&feed=atom&title=PET28b-ssfGFP PET28b-ssfGFP - Revision history 2026-10-18T05:36:24Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=PET28b-ssfGFP&diff=1166&oldid=prev Ruili.su at 20:56, 24 June 2011 2011-06-24T20:56:27Z <p></p> <p><b>New page</b></p><div>== &amp;nbsp;&#039;&#039;&#039;Gene Synthesis&amp;nbsp;of&amp;nbsp;ssfGFP to pET28b&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039;&amp;nbsp;Gene synthesis&amp;nbsp;of ssfGFP to pET28b <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2011-6-20 to 2011-6-24 <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; Robert <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; 001547 <br /> <br /> &#039;&#039;&#039;Clone ID:&#039;&#039;&#039; 169 <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Robert <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Gene Synthesis ===<br /> <br /> Date: 2011-6-23 <br /> <br /> Gene synthesis, mkate1 + ssfGFP19, using these mixture: ssfGFP1 <br /> <br /> ssfGFP2&lt;br&gt;ssfGFP3&lt;br&gt;ssfGFP4&lt;br&gt;ssfGFP5&lt;br&gt;ssfGFP6&lt;br&gt;ssfGFP7&lt;br&gt;ssfGFP8&lt;br&gt;ssfGFP9&lt;br&gt;ssfGFP10&lt;br&gt;ssfGFP11&lt;br&gt;ssfGFP12&lt;br&gt;ssfGFP13&lt;br&gt;ssfGFP14&lt;br&gt;ssfGFP15&lt;br&gt;ssfGFP16&lt;br&gt;ssfGFP17&lt;br&gt;ssfGFP18&lt;br&gt;ssfGFP19 <br /> <br /> Digest BamH1 + XhoI <br /> <br /> Ligate to BamH1 + XhoI digested pET28b <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2011-6-24 <br /> <br /> Make miniprep and digest with BamH1 + XhoI <br /> <br /> [[Image:SSgfp-062411.JPG]]&lt;br&gt; <br /> <br /> Lanes 1-6: pET28b, BamH1 + XhoI <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *Lane 6 has the correct number of bands.&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> Restriction digestion shows that clone #6 is good.&lt;br&gt; <br /> <br /> [[User:Paul.xu|Robert.Cai]]&amp;nbsp;02:48,&amp;nbsp;24 June 2011 (EST) <br /> <br /> [[Category:Cloning]]</div> Ruili.su