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PKA ELISA 4-5-2011 - Revision history
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Ztao at 20:35, 5 April 2011
2011-04-05T20:35:35Z
<p></p>
<p><b>New page</b></p><div>Notebook zhen tao #10<br />
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Page 2092<br />
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plate was coated with 2ug/ml PKA-C-alpha in TBS; 100 ul/well; then blocked by 1% BSA in TBS and 10% glycerol. each sample was diluted 1/10000 with blocking buffer. <br />
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<br> <br />
<br />
{| width="200" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| samples <br />
| OD450-1 <br />
| OD450-2 <br />
| OD450-3 <br />
| average<br />
|-<br />
| control <br />
| 0.025 <br />
| 0.021 <br />
| 0.019 <br />
| 0.022<br />
|-<br />
| 31 <br />
| 0.056 <br />
| 0.056 <br />
| 0.056 <br />
| 0.056<br />
|-<br />
| 32 <br />
| 0.078 <br />
| 0.080 <br />
| 0.080 <br />
| 0.079<br />
|-<br />
| 447673 <br />
| 0.110 <br />
| 0.122 <br />
| 0.155 <br />
| 0.129<br />
|-<br />
| 447674 <br />
| 0.054 <br />
| 0.059 <br />
| 0.056 <br />
| 0.056<br />
|-<br />
| positive control <br />
| 0.569 <br />
| 0.557 <br />
| 0.556 <br />
| 0.561<br />
|}<br />
--[[User:Ztao|Ztao]] 15:35, 5 April 2011 (EST)<br />
[[Category:ELISA]]</div>
Ztao