https://eln.excellgen.com/index.php?action=history&feed=atom&title=PKA_ELISA_4-6-2011 PKA ELISA 4-6-2011 - Revision history 2026-10-18T05:15:21Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=PKA_ELISA_4-6-2011&diff=613&oldid=prev Ztao at 19:28, 6 April 2011 2011-04-06T19:28:56Z <p></p> <p><b>New page</b></p><div>Notebook Zhen Tao #10&lt;br&gt; <br /> <br /> Page 2096&lt;br&gt; <br /> <br /> All samples are diluted 1/10000 with blocking solution (1%BSA, 10%glycerol in TBS).&lt;br&gt; <br /> <br /> Control: only 2nd antibody (anti-human) was used;&lt;br&gt;sample 31~40 are normal people serum;&lt;br&gt;sample 447667~447669: prostate cancer people serum;&lt;br&gt;sample 447670~447672: colon cancer people serum;&lt;br&gt;sample 447673~447674: breast cancer people serum.&lt;br&gt;&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 419px; height: 477px;&quot;<br /> |-<br /> | Samples&lt;br&gt; <br /> | OD450-1&lt;br&gt; <br /> | OD450-2&lt;br&gt; <br /> | OD450-3&lt;br&gt; <br /> | average&lt;br&gt; <br /> | average of normal serum sample<br /> |-<br /> | <br /> control&lt;br&gt; <br /> <br /> | 0.019&lt;br&gt; <br /> | 0.017&lt;br&gt; <br /> | 0.016&lt;br&gt; <br /> | 0.017&lt;br&gt;&lt;br&gt; <br /> | rowspan=&quot;8&quot; | 0.036<br /> |-<br /> | 31&lt;br&gt; <br /> | 0.031&lt;br&gt; <br /> | 0.033&lt;br&gt; <br /> | 0.034&lt;br&gt; <br /> | 0.033&lt;br&gt;<br /> |-<br /> | 32&lt;br&gt; <br /> | 0.044&lt;br&gt; <br /> | 0.043&lt;br&gt; <br /> | 0.043&lt;br&gt; <br /> | 0.043&lt;br&gt;<br /> |-<br /> | 33&lt;br&gt; <br /> | 0.037&lt;br&gt; <br /> | 0.037&lt;br&gt; <br /> | 0.045&lt;br&gt; <br /> | 0.040&lt;br&gt;<br /> |-<br /> | 34&lt;br&gt; <br /> | 0.029&lt;br&gt; <br /> | 0.035&lt;br&gt; <br /> | 0.046&lt;br&gt; <br /> | 0.037&lt;br&gt;<br /> |-<br /> | 35&lt;br&gt; <br /> | 0.043&lt;br&gt; <br /> | 0.042&lt;br&gt; <br /> | 0.040&lt;br&gt; <br /> | 0.042&lt;br&gt;<br /> |-<br /> | 39&lt;br&gt; <br /> | 0.032&lt;br&gt; <br /> | 0.033&lt;br&gt; <br /> | 0.032&lt;br&gt; <br /> | 0.032&lt;br&gt;<br /> |-<br /> | 40&lt;br&gt; <br /> | 0.027&lt;br&gt; <br /> | 0.024&lt;br&gt; <br /> | 0.026&lt;br&gt; <br /> | 0.026&lt;br&gt;<br /> |-<br /> | 447667&lt;br&gt; <br /> | 0.108&lt;br&gt; <br /> | 0.094&lt;br&gt; <br /> | 0.107&lt;br&gt; <br /> | 0.103&lt;br&gt; <br /> | <br /> |-<br /> | 447668&lt;br&gt; <br /> | 0.087&lt;br&gt; <br /> | 0.077&lt;br&gt; <br /> | 0.082&lt;br&gt; <br /> | 0.082&lt;br&gt; <br /> | <br /> |-<br /> | 447669&lt;br&gt; <br /> | 0.039&lt;br&gt; <br /> | 0.036&lt;br&gt; <br /> | 0.030&lt;br&gt; <br /> | 0.035&lt;br&gt; <br /> | <br /> |-<br /> | 447670&lt;br&gt; <br /> | 0.051&lt;br&gt; <br /> | 0.045&lt;br&gt; <br /> | 0.042&lt;br&gt; <br /> | 0.046&lt;br&gt; <br /> | <br /> |-<br /> | 447471&lt;br&gt; <br /> | 0.030&lt;br&gt; <br /> | 0.031&lt;br&gt; <br /> | 0.027&lt;br&gt; <br /> | 0.029&lt;br&gt; <br /> | <br /> |-<br /> | 447672&lt;br&gt; <br /> | 0.035&lt;br&gt; <br /> | 0.035&lt;br&gt; <br /> | 0.039&lt;br&gt; <br /> | 0.036&lt;br&gt; <br /> | <br /> |-<br /> | 447673&lt;br&gt; <br /> | 0.058&lt;br&gt; <br /> | 0.063&lt;br&gt; <br /> | 0.054&lt;br&gt; <br /> | 0.058&lt;br&gt; <br /> | <br /> |-<br /> | 447674&lt;br&gt; <br /> | 0.023&lt;br&gt; <br /> | 0.025&lt;br&gt; <br /> | 0.026&lt;br&gt; <br /> | 0.025&lt;br&gt; <br /> | <br /> |-<br /> | positive control&lt;br&gt; <br /> | 0.345&lt;br&gt; <br /> | 0.339&lt;br&gt; <br /> | 0.342&lt;br&gt; <br /> | 0.342&lt;br&gt; <br /> | <br /> |}<br /> <br /> &lt;br&gt;--[[User:Ztao|Ztao]] 14:26, 6 April 2011 (EST) <br /> <br /> [[Category:ELISA]]</div> Ztao