https://eln.excellgen.com/index.php?action=history&feed=atom&title=PKA_ELISA_4-7-2011 PKA ELISA 4-7-2011 - Revision history 2026-10-18T07:25:24Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=PKA_ELISA_4-7-2011&diff=616&oldid=prev Ztao at 18:32, 7 April 2011 2011-04-07T18:32:51Z <p></p> <p><b>New page</b></p><div>Notebook Zhen Tao #10&lt;br&gt; <br /> <br /> Page 2097~2098&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> all samples were diluted 1/2000 with blocking solution;&lt;br&gt;Control: only 2nd antibody (anti-human) was used;&lt;br&gt;sample 31~40: normal people serum;&lt;br&gt;sample 447667~447669: prostate cancer people serum;&lt;br&gt;sample 447670~447672: colon cancer people serum;&lt;br&gt;sample 447673~447674: breast cancer people serum;&lt;br&gt;positive: anti-PKA-C-alpha was used (1/10000)<br /> <br /> <br /> <br /> OD450 data for normal and cancer samples are corrected by substrating the average of control (0.018).&lt;br&gt;&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 550px; height: 434px;&quot;<br /> |-<br /> | samples&lt;br&gt; <br /> | OD450-1&lt;br&gt; <br /> | OD450-2&lt;br&gt; <br /> | OD450-3&lt;br&gt; <br /> | average of normal serum&lt;br&gt; <br /> | cancer/normal&lt;br&gt;<br /> |-<br /> | control&lt;br&gt; <br /> | 0.017&lt;br&gt; <br /> | 0.018&lt;br&gt; <br /> | 0.020&lt;br&gt; <br /> | 0.018&lt;br&gt; <br /> | rowspan=&quot;8&quot; | &lt;br&gt;<br /> |-<br /> | 31&lt;br&gt; <br /> | 0.107&lt;br&gt; <br /> | 0.098&lt;br&gt; <br /> | 0.109&lt;br&gt; <br /> | rowspan=&quot;7&quot; | 0.124&lt;br&gt;<br /> |-<br /> | 32&lt;br&gt; <br /> | 0.169&lt;br&gt; <br /> | 0.157&lt;br&gt; <br /> | 0.165&lt;br&gt;<br /> |-<br /> | 33&lt;br&gt; <br /> | 0.144 &lt;br&gt; <br /> | 0.133&lt;br&gt; <br /> | 0.152&lt;br&gt;<br /> |-<br /> | 34&lt;br&gt; <br /> | 0.092 &lt;br&gt; <br /> | 0.126&lt;br&gt; <br /> | 0.071&lt;br&gt;<br /> |-<br /> | 35&lt;br&gt; <br /> | 0.154 &lt;br&gt; <br /> | 0.204&lt;br&gt; <br /> | 0.159&lt;br&gt;<br /> |-<br /> | 39&lt;br&gt; <br /> | 0.122 &lt;br&gt; <br /> | 0.130&lt;br&gt; <br /> | 0.098&lt;br&gt;<br /> |-<br /> | 40&lt;br&gt; <br /> | 0.065 &lt;br&gt; <br /> | 0.087&lt;br&gt; <br /> | 0.077&lt;br&gt;<br /> |-<br /> | 447667&lt;br&gt; <br /> | 0.499 &lt;br&gt; <br /> | 0.650&lt;br&gt; <br /> | 0.463&lt;br&gt; <br /> | &lt;br&gt; <br /> | 4.317&lt;br&gt;<br /> |-<br /> | 447668&lt;br&gt; <br /> | 0.387&lt;br&gt; <br /> | 0.492&lt;br&gt; <br /> | 0.346&lt;br&gt; <br /> | &lt;br&gt; <br /> | 3.280&lt;br&gt;<br /> |-<br /> | 447669&lt;br&gt; <br /> | 0.170 &lt;br&gt; <br /> | 0.257&lt;br&gt; <br /> | 0.117&lt;br&gt; <br /> | &lt;br&gt; <br /> | 1.455&lt;br&gt;<br /> |-<br /> | 447670&lt;br&gt; <br /> | 0.232 &lt;br&gt; <br /> | 0.246&lt;br&gt; <br /> | 0.188&lt;br&gt; <br /> | &lt;br&gt; <br /> | 1.782&lt;br&gt;<br /> |-<br /> | 447671&lt;br&gt; <br /> | 0.077&lt;br&gt; <br /> | 0.082&lt;br&gt; <br /> | 0.080&lt;br&gt; <br /> | &lt;br&gt; <br /> | 0.638&lt;br&gt;<br /> |-<br /> | 447672&lt;br&gt; <br /> | 0.405 &lt;br&gt; <br /> | 0.433&lt;br&gt; <br /> | 0.410&lt;br&gt; <br /> | &lt;br&gt; <br /> | 3.342&lt;br&gt;<br /> |-<br /> | 447673&lt;br&gt; <br /> | 0.274 &lt;br&gt; <br /> | 0.281&lt;br&gt; <br /> | 0.258&lt;br&gt; <br /> | &lt;br&gt; <br /> | 2.176&lt;br&gt;<br /> |-<br /> | 447674&lt;br&gt; <br /> | 0.095 &lt;br&gt; <br /> | 0.095&lt;br&gt; <br /> | 0.101&lt;br&gt; <br /> | &lt;br&gt; <br /> | 0.777&lt;br&gt;<br /> |-<br /> | postitive&lt;br&gt; <br /> | 0.964 &lt;br&gt; <br /> | 0.976&lt;br&gt; <br /> | 0.989&lt;br&gt; <br /> | &lt;br&gt; <br /> | 7.847&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt;--[[User:Ztao|Ztao]] 13:29, 7 April 2011 (EST) <br /> <br /> [[Category:ELISA]]</div> Ztao