https://eln.excellgen.com/index.php?action=history&feed=atom&title=PKA_ELISA_4-8-2011 PKA ELISA 4-8-2011 - Revision history 2026-10-18T06:11:12Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=PKA_ELISA_4-8-2011&diff=619&oldid=prev Ztao at 18:24, 8 April 2011 2011-04-08T18:24:15Z <p></p> <p><b>New page</b></p><div>Notebook Zhen Tao #11&lt;br&gt; <br /> <br /> Page 2101~2102&lt;br&gt; <br /> <br /> all samples are diluted 1/400 with blocking solution;&lt;br&gt;Control: only 2nd antibody (anti-human) was used;&lt;br&gt;sample 29 and 447667~447669: prostate cancer people serum;&lt;br&gt;sample 447670~447672: colon cancer people serum;&lt;br&gt;sample 447673~447675: breast cancer people serum;&lt;br&gt; <br /> <br /> sample 31~146 are normal people serum;&lt;br&gt;positive: anti-PKA-C-alpha is used <br /> <br /> 15 min, instead of 30 min was used here after adding TMB solution.&lt;br&gt;&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 752px; height: 555px;&quot;<br /> |-<br /> | samples&lt;br&gt; <br /> | OD450-1&lt;br&gt; <br /> | OD450-2&lt;br&gt; <br /> | OD450-3&lt;br&gt; <br /> | average of all normal serum&lt;br&gt; <br /> | average of cancer sample&lt;br&gt; <br /> | ration of cancer/normal&lt;br&gt;<br /> |-<br /> | control&lt;br&gt; <br /> | 0.005&lt;br&gt; <br /> | 0.006&lt;br&gt; <br /> | 0.005&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 447667&lt;br&gt; <br /> | 0.856&lt;br&gt; <br /> | 0.841&lt;br&gt; <br /> | 0.850&lt;br&gt; <br /> | &lt;br&gt; <br /> | 0.844&lt;br&gt; <br /> | 3.706&lt;br&gt;<br /> |-<br /> | 447668&lt;br&gt; <br /> | 0.501&lt;br&gt; <br /> | 0.508&lt;br&gt; <br /> | 0.526&lt;br&gt; <br /> | &lt;br&gt; <br /> | 0.506&lt;br&gt; <br /> | 2.224&lt;br&gt;<br /> |-<br /> | 447669&lt;br&gt; <br /> | 0.323&lt;br&gt; <br /> | 0.331&lt;br&gt; <br /> | 0.309&lt;br&gt; <br /> | &lt;br&gt; <br /> | 0.316&lt;br&gt; <br /> | 1.387&lt;br&gt;<br /> |-<br /> | 447670&lt;br&gt; <br /> | 0.290&lt;br&gt; <br /> | 0.289&lt;br&gt; <br /> | 0.290&lt;br&gt; <br /> | &lt;br&gt; <br /> | 0.284&lt;br&gt; <br /> | 1.249&lt;br&gt;<br /> |-<br /> | 447671&lt;br&gt; <br /> | 0.143&lt;br&gt; <br /> | 0.146&lt;br&gt; <br /> | 0.152&lt;br&gt; <br /> | &lt;br&gt; <br /> | 0.142&lt;br&gt; <br /> | 0.622&lt;br&gt;<br /> |-<br /> | 447672&lt;br&gt; <br /> | 0.468&lt;br&gt; <br /> | 0.505&lt;br&gt; <br /> | 0.547&lt;br&gt; <br /> | &lt;br&gt; <br /> | 0.501&lt;br&gt; <br /> | 2.202&lt;br&gt;<br /> |-<br /> | 447673&lt;br&gt; <br /> | 0.895&lt;br&gt; <br /> | 0.545&lt;br&gt; <br /> | 0.585&lt;br&gt; <br /> | &lt;br&gt; <br /> | 0.670&lt;br&gt; <br /> | 2.942&lt;br&gt;<br /> |-<br /> | 447674&lt;br&gt; <br /> | 0.153&lt;br&gt; <br /> | 0.160&lt;br&gt; <br /> | 0.169&lt;br&gt; <br /> | &lt;br&gt; <br /> | 0.155&lt;br&gt; <br /> | 0.682&lt;br&gt;<br /> |-<br /> | 31&lt;br&gt; <br /> | 0.190&lt;br&gt; <br /> | 0.193&lt;br&gt; <br /> | 0.201&lt;br&gt; <br /> | rowspan=&quot;12&quot; | 0.228&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 32&lt;br&gt; <br /> | 0.279&lt;br&gt; <br /> | 0.281&lt;br&gt; <br /> | 0.318&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 33&lt;br&gt; <br /> | 0.232&lt;br&gt; <br /> | 0.255&lt;br&gt; <br /> | 0.242&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 34&lt;br&gt; <br /> | 0.177&lt;br&gt; <br /> | 0.168&lt;br&gt; <br /> | 0.151&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 35&lt;br&gt; <br /> | 0.412&lt;br&gt; <br /> | 0.393&lt;br&gt; <br /> | 0.418&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 39&lt;br&gt; <br /> | 0.205&lt;br&gt; <br /> | 0.208&lt;br&gt; <br /> | 0.211&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 40&lt;br&gt; <br /> | 0.106&lt;br&gt; <br /> | 0.124&lt;br&gt; <br /> | 0.124&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 132&lt;br&gt; <br /> | 0.285&lt;br&gt; <br /> | 0.286&lt;br&gt; <br /> | 0.260&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 133&lt;br&gt; <br /> | 0.167&lt;br&gt; <br /> | 0.172&lt;br&gt; <br /> | 0.182&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 135&lt;br&gt; <br /> | 0.151&lt;br&gt; <br /> | 0.153&lt;br&gt; <br /> | 0.166&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 136&lt;br&gt; <br /> | 0.058&lt;br&gt; <br /> | 0.590&lt;br&gt; <br /> | 0.156&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 146&lt;br&gt; <br /> | 0.313&lt;br&gt; <br /> | 0.291&lt;br&gt; <br /> | 0.269&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | positive&lt;br&gt; <br /> | 1.718&lt;br&gt; <br /> | 1.824&lt;br&gt; <br /> | 1.869&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt;--[[User:Ztao|Ztao]] 13:03, 8 April 2011 (EST)&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Category:ELISA]]</div> Ztao