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PKA ELISA 6-20-11 - Revision history
2026-10-18T14:09:45Z
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Hylei at 18:30, 22 June 2011
2011-06-22T18:30:20Z
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<p><b>New page</b></p><div><tab border="1"><br />
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1 2 3 4 5 6 7 8 9 10 11 12<br />
0.468 0.451 0.368 0.361 0.216 0.219 0.137 0.135 0.129 0.127 0.134 0.103<br />
0.219 0.213 0.281 0.288 0.178 0.181 0.114 0.119 0.123 0.122 0.099 0.1<br />
0.437 0.419 0.373 0.399 0.227 0.231 0.136 0.141 0.134 0.138 0.101 0.101<br />
0.275 0.281 0.322 0.333 0.195 0.194 0.113 0.118 0.138 0.125 0.101 0.097<br />
0.279 0.266 0.333 0.288 0.258 0.264 0.126 0.14 0.126 0.13 0.103 0.093<br />
0.302 0.296 0.256 0.248 0.178 0.177 0.147 0.155 0.17 0.166 0.102 0.101<br />
0.191 0.205 0.211 0.213 0.148 0.157 0.136 0.128 0.146 0.145 0.087 0.091<br />
0.228 0.23 0.237 0.237 0.206 0.216 0.142 0.138 0.228 0.15 0.101 0.098<br />
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</tab> <br />
<br />
Line 1,2: 20ug/ml pka in 0.1M sodium bicarbonate;<br />
line 1, pierce blocking buffer; line 2, pbs/2%bsa blocking buffer;<br />
<br />
Line 3,4,5, 10ug/ml pka; <br />
line 3, pierce blocking buffer; line 4, pbs/2%bsa blocking buffer; line 5, blocking buffer with glycerol.<br />
line 6,7,8 2ug/ml pka;<br />
line 6, pierce blocking buffer; line 7, pbs/2%bsa blocking buffer; line 8, blocking buffer with glycerol.<br />
<br />
1,2 673; 3,4, 675; 5,6, 670; 7,8, 40; 9,10, 135; 11, 12 control.<br />
lab note 2933.<br />
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<br />
10ug/ml plate-06/21/2011<br />
<tab border="1"><br />
<br />
1 2 3 4 5 6 7 8 9 10 11 12<br />
0.467 0.488 0.647 0.43 0.469 0.448 0.833 0.872 0.851 0.905 0.273 0.194<br />
0.26 0.197 0.141 0.151 0.127 0.139 0.165 0.209 0.117 0.136 0.108 0.112<br />
0.125 0.144 0.153 0.173 0.148 0.192 0.203 0.225 0.29 0.305 0.097 0.069<br />
0.742 0.746 0.542 0.572 0.459 0.497 1.34 1.308 1.449 1.466 0.311 0.214<br />
0.493 0.296 0.167 0.157 0.142 0.131 0.241 0.232 0.186 0.182 0.243 0.231<br />
0.192 0.166 0.198 0.246 0.182 0.181 0.254 0.248 0.259 0.266 0.093 0.081<br />
1.832 1.703 2.539 2.722 1.111 1.512 1.058 1.005 1.839 0.98 0.439 0.564<br />
2.567 2.409 2.711 3.011 1.441 1.819 0.994 0.954 1.806 1.79 0.395 0.483<br />
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</tab> <br />
<br />
<br />
2ug/ml plate-06/21/2011<br />
<tab border="1"><br />
<br />
1 2 3 4 5 6 7 8 9 10 11 12<br />
0.303 0.29 0.572 0.468 0.383 0.373 0.857 0.901 0.795 0.827 0.227 0.215<br />
0.198 0.242 0.131 0.124 0.105 0.112 0.209 0.218 0.122 0.125 0.109 0.114<br />
0.127 0.151 0.13 0.124 0.14 0.132 0.2 0.262 0.186 0.214 0.095 0.1<br />
'''0.547 0.486 0.525 0.569 0.382 0.392 1.163 1.213 1.374 1.457 0.245 0.282<br />
0.298 0.299 0.163 0.173 0.134 0.191 0.228 0.24 0.21 0.181 0.197 0.195<br />
0.172 0.157 0.139 0.145 0.199 0.178 0.246 0.226 0.242 0.376 0.074 0.075'''<br />
1.309 1.595 2.106 1.832 0.907 1.215 0.943 1.11 1.098 1.128 0.521 0.553<br />
1.89 1.951 2.564 2.762 1.538 1.91 1.18 1.205 1.764 1.593 0.536 0.564<br />
<br />
</tab> <br />
<br />
For the 10ug/ml and 2ug/ml plates, duplicate the sample, from a1 to c12, the template is: 670, 672, 939, 941, 942, 940, 935, 929, 930, 933, 671, 35, 135, 40, 136, 943, 936, control; 1:1000 dilution; for the d1 to f12, template order is same, 1:500 dilution; g1, g2 is the test for the new secondary ab, 1:5000 dilution, 200ng/ml, seems too strong, next time needs to more dilution.<br />
<br />
Conclusion: 2ug/ml coating plates; compare with 06/16/11 data, for the 1:1000 dilution, 940, 935, 943, 936 previous data read is between 0.15 to 0.2, not sure positive, now all above 0.2, can consider positive; for the 929, 930, 933, 671 previous can consider negative, still below 0.2, can consider negative; for the 35, 135, 40, 136, all below 0.2.<br />
<br />
next time condition is: 2ug/ml coating plates, serum 1:500 dilution, reader above 0.2 can think positive.<br />
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[[Category:ELISA]]</div>
Hylei