Ztao: Created page with "{| cellspacing="1" cellpadding="1" border="1" style="width: 360px; height: 282px;" |- | sample | OD450-1 | OD450-2 | OD450-3 | Average |- | 31 | 0.118 | 0.128 | 0.125 | ..."

2011-03-28T21:56:16Z

Created page with "{| cellspacing="1" cellpadding="1" border="1" style="width: 360px; height: 282px;" |- | sample | OD450-1 | OD450-2 | OD450-3 | Average |- | 31 | 0.118 | 0.128 | 0.125 | ..."

New page

{| cellspacing="1" cellpadding="1" border="1" style="width: 360px; height: 282px;"
|-
|
sample

| OD450-1
| OD450-2
| OD450-3
| Average
|-
| 31
| 0.118
| 0.128
| 0.125
| 0.124
|-
| 32
| 0.147
| 0.151
| 0.147
| 0.148
|-
| 33
| 0.125
| 0.129
| 0.123
| 0.126
|-
| 34
| 0.123
| 0.130
| 0.134
| 0.129
|-
| 447673
| 0.203
| 0.226
| 0.237
| 0.222
|-
| 447674
| 0.122
| 0.125
| 0.126
| 0.124
|-
| 447675
| 0.151
| 0.159
| 0.158
| 0.156
|-
| positive control
| 0.398
| 0.408
| 0.381
| 0.396
|-
| control
| 0.089
| 0.099
| 0.097
| 0.095
|}

Plate is coated with PKA-C alpha with OD280 = 0.075;

Blocked by using 1%BSA, 10% glycerol in TBS;

Serum or 1st antibody is diluted 10,000 times with blocking buffer;

smaples 31~34 are serum from normal people; samples 447673~75 are from breast cancer people; positive control is anti-PKA-C alpha; control: no serum or 1st antibody was used, only 2nd antibody is used. 

 --[[User:Ztao|Ztao]] 14:19, 25 March 2011 (EST)

 

 

 

 

[[Category:ELISA]]

PKA ELISA dev 1 - Revision history

Ztao: Created page with "{| cellspacing="1" cellpadding="1" border="1" style="width: 360px; height: 282px;" |- | sample | OD450-1 | OD450-2 | OD450-3 | Average |- | 31 | 0.118 | 0.128 | 0.125 | ..."