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PKA alpha pSpeedET-purification - Revision history
2026-10-18T10:20:57Z
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Ruili.su at 20:19, 7 February 2012
2012-02-07T20:19:45Z
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<p><b>New page</b></p><div>[[Image:PKA alpha-PspeedET-02-04-2012.jpg|275x274px|PKA alpha-PspeedET-02-04-2012.jpg]] <br />
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Notebook: Ruili Su 2<br> <br />
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Page:003504--003505<br> <br />
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Date:01/27/2012--02/01/2012<br> <br />
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PKAalpha-pSpeedET transformed with C43(DE3)PlySs and after induced with 1ml 20% Arabinose/L and growing 3-4hours at <br> <br />
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35 <sup>0</sup>C. Run FPLC.<br> <br />
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After FPLC, loading 15ul sample/well for SDS-PAGE.<br> <br />
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Line1: Flow through<br> <br />
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Line2--- eluted PKA.<br> <br />
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[[Image:PKA alpha-PspeedET-02-05-2012.jpg|275x273px|PKA alpha-PspeedET-02-05-2012.jpg]] <br />
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Date: 02/02/2012--02/04/2012<br> <br />
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Page: 003506--003507<br> <br />
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PKAalpha-pSpeedET transformed with C41(DE3)PlySs. Culturing cocndition is same as PKAalpha-pSpeedET transformed with C43(DE3)PlySs. <br> <br />
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Run SDS-PAGE to check size and purity. <br />
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Line 1: Flow through (15ul) <br />
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Line 2----: Eluted PKA after FPLC (15ul/well). <br />
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'''Result'''<br> <br />
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PKAalpha-sPeedET has protein expression after transformed with C41(DE3)PlySs and C43(DE3)PlySs, but the expression level is not good.<br />
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[[Category:Protein_Purification]]</div>
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