Ruili.su at 19:20, 25 July 2011

2011-07-25T19:20:04Z

New page

Use new protocol and Qiagen column (5 ml) [[PKA Purification]]

 

[[Image:Pka alpha purification qiagen column.jpg]]

Run SDS-PAGE

10  ul Fraction 2 to 10, ---> lane 1 to 9 see notebook page 2930-2932;

combine fraction 2 to 6, use pbs/10% glycerol dialysis o/n;

before dialysis, od 280 is 0.410; afer dialysis, od 280 is 0.504;

estimate protein concentration is around 0.5mg/ml.

aliquot to 1.5ml tube, freeze in -80 degree (in the 0.1mg/ml pka, 10%glycerol box, use the red marker).

 --[[User:Paul.xu, haiyan lei|Paul.xu]] 16:16, 19 June 2011 (EST)

 

[[Image:PKA-alpha_purification-7.07.11.jpg]] 

20 ul samples from fraction 1 through 9 (note book 002801, Ruili Su); 

Combine faction 1 to 9, use PBS with20% glycerol to dialysis O/N; 

Then adjust the protein concerntration until 1.5mg/ml by centrifuge. 

--[[User:Ruili.su|Ruili.su]] 14:02, 25 July 2011 (EST)

[[Category:Protein_Purification]]

PKA alpha purification 2011 6 17 - Revision history

Ruili.su at 19:20, 25 July 2011