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PKA alpha purification Dec 2012 - Revision history
2026-10-18T03:04:21Z
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Paul.xu at 23:50, 4 December 2012
2012-12-04T23:50:59Z
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<p><b>New page</b></p><div>1. Nov 2012 <br />
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Purify using Ni column <br />
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[[Image:2012 11 Flu PKAalpha.jpg]] <br />
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2. Nov 29 2012 <br />
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From 8 L: Total wet cell: 30 g<br />
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Induction: 1 ml of 20% L-arabinose, 37oC o/n<br />
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Purify using 15 ml Ni column, elute protein with 300 mM&nbsp;Imidazole, concentrate using Pierce 10k spin column,&nbsp; then load to gel filtration column Bio-Rad BioSil TSK-250. Program is 47. injection: 5 ml, 2.5 ml/min, 4 ml per fraction.<br><br />
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[[Image:PKA alpha gel filtration 12 2012.jpg]] <br />
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[[Image:PKAalpha 2012 12 1.jpg]] <br />
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{| width="200" cellspacing="1" cellpadding="1" border="1"<br />
|-<br />
| Fraction<br />
| Protein Conc mg/ml<br />
|-<br />
| 34<br />
| 0.2<br />
|-<br />
| 35<br />
| 2.9<br />
|-<br />
| 36<br />
| 2.86<br />
|-<br />
| 37<br />
| 2.88<br />
|-<br />
| 38<br />
| 1.62<br />
|-<br />
| 39<br />
| 0.53<br />
|-<br />
| 40<br />
| 0.28<br />
|}<br />
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Store protein at -80 oC<br />
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[[Category:Protein_Purification]]</div>
Paul.xu