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PLVGP - Revision history
2026-10-18T03:25:08Z
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Paul.xu at 16:13, 7 June 2011
2011-06-07T16:13:43Z
<p></p>
<p><b>New page</b></p><div>Cloning Method <br />
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to PCR out GreenPuro<br> Xho1 22nt IRES<br> atcg CTCGAG TTTATAATTTCTTCTTCCAGAA GCCACCatggagagcgacg <br />
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GFP-Puro-F<br>atcgCTCGAGTTTATAATTTCTTCTTCCAGAAGCCACCatggagagcgacg<br>GFP-Puro-R Mlu1<br>cccgcaagcccggtgcctga ACGCGT atcg<br>oligo <br />
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cgatACGCGTtcaggcaccgggcttgcggg <br />
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<br>PCR out using pGreenPuro as template, digest with Xho1+ Mlu1, ligate with Xho1+ Mlu1 digested pLVX-IRES-mCherry-Plus2[[Image:PLentiGP-PKA-alpha-pLVGP_6_7_2011.JPG]]<br><br />
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Lane 5: Clone #1 digest with Xho1+ Mlu1<br><br />
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Lane 6: Clone #2 digest with Xho1+ Mlu1<br><br />
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Lane 7: Clone #3 digest with Xho1+ Mlu1<br><br />
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Lane 8: Clone #4 digest with Xho1+ Mlu1<br><br> <br />
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[[Category:Vectors]]</div>
Paul.xu