https://eln.excellgen.com/index.php?action=history&feed=atom&title=PLenti-mKate2
PLenti-mKate2 - Revision history
2026-10-18T07:59:59Z
Revision history for this page on the wiki
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https://eln.excellgen.com/index.php?title=PLenti-mKate2&diff=1140&oldid=prev
Ruili.su at 15:49, 24 June 2011
2011-06-24T15:49:34Z
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<p><b>New page</b></p><div>== '''SubCloning of mkate2 to pLentiGP''' ==<br />
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'''Purpose:''' Subcloning of mkate2 to pLentiGP from pet28b-mkate2. <br />
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'''Date: '''2011-6-20 to 2011-6-23 <br />
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'''Notebook:''' David, Robert <br />
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'''Page:''' 001543 <br />
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'''Clone ID:''' 220 <br />
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'''Experimenters:''' David, Robert, Haiyan http://192.168.1.11/eln/index.php?title=PLentiGP-PKA-Alpha&amp;action=edit <br />
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== '''Results''' ==<br />
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=== Subcloning ===<br />
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Date: 2011-6-23<br> <br />
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Digest pET28b-mKate2 with BamH1 + Xho1<br> <br />
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Run gel and cut out insert <br />
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Digest pLentiPlus with BamH1 + Xho1 <br />
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Run gel and cut out vector combine and purify using spin column <br />
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Ligate and transform STBL3 <br />
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=== Colony Screening ===<br />
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Date: 2011-6-23 <br />
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Make miniprep and digest pLenti-mKate2 with BamHI + Xho1 <br />
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<br> [[Image:PLentimKate2-6-23-11.JPG]] <br />
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Lanes 1 - 11: pLenti-mKate2, BamHI + Xho1<br> <br />
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== '''Result intepretation/Summary''' ==<br />
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*Lane 4 has the correct number of bands.<br><br />
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== '''Conclusion''' ==<br />
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Restriction digestion shows that clone #4 is good.<br> <br />
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=== ===<br />
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[[User:David.Hu|David.Hu]] 11:33, 23 June 2011 (EST)<br> <br />
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[[Category:Cloning]]</div>
Ruili.su