https://eln.excellgen.com/index.php?action=history&feed=atom&title=PTr2-CB-SMN1 PTr2-CB-SMN1 - Revision history 2026-10-18T10:26:37Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=PTr2-CB-SMN1&diff=1211&oldid=prev Ruili.su at 20:15, 28 June 2011 2011-06-28T20:15:32Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of SMN1 to PTr2-CB&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; cloning of SMN1 to PTr2-CB&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2011-6-24 <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; Ruili Su&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; 002785--002786 <br /> <br /> &#039;&#039;&#039;Clone ID:&#039;&#039;&#039; 255 <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Ruili <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Gene Synthesis &lt;br&gt; ===<br /> <br /> Date: 2011-6-24 <br /> <br /> Gene Synthesis oligos (SMN1 throuth SMN12)&lt;br&gt; <br /> <br /> &lt;br&gt; [[Image:PTr2-CB-SMN1-06-24-11-1.JPG]] <br /> <br /> &amp;lt;Picture 1&amp;gt;&lt;br&gt;<br /> <br /> 1. Marker; <br /> <br /> 3. Synthesized gene from PCR (10 ul synthesized gene).&lt;br&gt;<br /> <br /> &lt;br&gt; <br /> <br /> &#039;&#039;&#039;Subcloning&#039;&#039;&#039; <br /> <br /> Date: 2011-6-24 <br /> <br /> Digest pTr2-CB (244) with HindIII + XhoI &lt;br&gt; <br /> <br /> Digest the synthesized gene with HindIII + XhoI &lt;br&gt; <br /> <br /> cut out insert and vector, ligate and transform STBL3 &lt;br&gt;<br /> <br /> [[Image:PTr2-CB-SMN1-06-24-11-2.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;Picture 2&amp;gt;&lt;br&gt;1. The digested vector PTr2-CB (244) &lt;br&gt; <br /> <br /> 2 through 4. the digested synthesized gene (404bp) &lt;br&gt; <br /> <br /> 5. Marker.&lt;br&gt; <br /> <br /> Date: 2011-6-27 &lt;br&gt; <br /> <br /> [[Image:PTr2-CB-SMN1-06-27-11-3.JPG]]&lt;br&gt; <br /> <br /> &amp;lt;Picture 3&amp;gt;.&lt;br&gt;<br /> <br /> 1. Marker.&lt;br&gt; <br /> <br /> 2. Digested pAAV-GFP (247)&amp;nbsp; with EcoRI + Xhol.&lt;br&gt; <br /> <br /> 3. Digested PTr2-CB(244)with HindIII. &lt;br&gt;<br /> <br /> 4. Digested PTr2-CB(244)with XHoI. &lt;br&gt;<br /> <br /> 5. Digested PTr2-CB(244) with HindIII + XHoI (Fast digestion buffer). &lt;br&gt;<br /> <br /> 6. Digested PTr2-CB(244) with HindIII + XHoI (NEB Buffer). &lt;br&gt;<br /> <br /> 7. Undigested PTr2-CB(244) <br /> <br /> [[Image:PTr2-CB-SMN1-06-27-11-4.JPG]]&lt;br&gt;<br /> <br /> &amp;lt;Picture 4&amp;gt;&lt;br&gt;<br /> <br /> 1. Marker.&lt;br&gt;<br /> <br /> 2---7 Samples have nothing to do with this cloning.&lt;br&gt;<br /> <br /> 8. Digested PTr2-CB(244) with HindIII + XHoI (Fast digestion buffer).&lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;*Sequencing Result:&amp;lt;br&amp;gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> Restriction digestion &amp;lt;br&amp;gt; <br /> <br /> &lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> &lt;br&gt; <br /> <br /> &amp;lt;br&amp;gt; <br /> <br /> === ===<br /> <br /> --[[User:Ruili.su|Ruili.su]] 13:34, 24 June 2011 (EST) <br /> <br /> [[Category:Cloning]]</div> Ruili.su