Paul.xu at 14:39, 4 February 2011

2011-02-04T14:39:49Z

New page

1 Day after transfection 

[[Image:Lentivirus vector transfection optimization.JPG]]

 

[[Image:Lentivirus packaging mix optimization day 2.JPG]]

2 days after transfection

{| cellspacing="1" cellpadding="1" border="1" style="width: 609px; height: 242px;"
|-
| 
| Vector 
| Packaging mix 
| Transfection Reagent 
|-
| 1 
| - 
| - 
| - 
|-
| 2 
| - 
| Sigma 
| PolyExpress 
|-
| 3 
| pLL3.7 
| Sigma 
| PolyExpress 
|-
| 4 
| pLL3.7 
| 1:1 
| PolyExpress 
|-
| 5 
| pLL3.7 
| 1:3 
| PolyExpress 
|-
| 6 
| pLL3.7 
| 1:5 
| PolyExpress 
|-
| 7 
| pLL3.7 
| 1:10 
| PolyExpress 
|-
| 8 
| pSMPUW-EGFP 
| Sigma 
| PolyExpress 
|-
| 9 
| pSMPUW-EGFP 
| 1:3 
| PolyExpress 
|-
| 10 
| pSMPUW-EGFP 
| Sigma 
| PolyExpress 
|-
| 11 
| pSMPUW-EGFP 
| 1:3 
| FuGene 
|-
| 12 
| pSMPUW-EGFP 
| Sigma 
| FuGene 
|}

 

Fugene: 1.2 ul 

PolyExpress: 4.5 ul

pLL3.7:0.2 ug (1ul of 200 ng/ul) 

pSMPUW-EGFP: 0.2 ug (1ul of 200 ng/ul) 

Packaging mix: 2 ul (Sigma or homemade, pVSVG/pGag-Pol)

[[Image:Packagingmix optimization 1 infection day1.JPG]] 

add 1 ml conditioned medium (containinng lentivirus) to cells

1 day post infection --[[User:Paul.xu|Paul.xu]] 12:54, 29 January 2011 (EST)

 [[Image:Packagingmix optimization 1 day3 post infection.JPG]]

2 days post infection 

 

--[[User:Paul.xu|Paul.xu]] 10:46, 30 January 2011 (EST)

[[Image:Lentivirus packaging mix optimizationg 1 infection day3.JPG]]

3 days post infection --[[User:Paul.xu|Paul.xu]] 11:13, 1 February 2011 (EST)

 

[[Image:Lentivirus packaging mix optimization 1 day 5 post infection.JPG]]

5 days post infection

--[[User:Paul.xu|Paul.xu]] 10:49, 3 February 2011 (EST)

 

[[Image:Lentivirus packaging mix optimization 1 day 6 post infection.JPG]]

6 days post infection

--[[User:Paul.xu|Paul.xu]] 09:39, 4 February 2011 (EST)

[[Category:Lentivirus]]

Packagingmix optimization 1 - Revision history

Paul.xu at 14:39, 4 February 2011