https://eln.excellgen.com/index.php?action=history&feed=atom&title=Packagingmix_optimization_2 Packagingmix optimization 2 - Revision history 2026-10-18T08:38:00Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Packagingmix_optimization_2&diff=199&oldid=prev Paul.xu at 19:23, 5 February 2011 2011-02-05T19:23:47Z <p></p> <p><b>New page</b></p><div>Notebook # <br /> <br /> Page #: 001430 <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:Lentivirus packaging mix optimizationg 2 day1.JPG]] &lt;br&gt; <br /> <br /> 1 Day after transfection &lt;br&gt; &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:Packagingmix optimization 2 day2.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> 2 days after transfection <br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 609px; height: 242px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | Vector&lt;br&gt; <br /> | Packaging mix&lt;br&gt; <br /> | Transfection Reagent&lt;br&gt;<br /> |-<br /> | 1&lt;br&gt; <br /> | -&lt;br&gt; <br /> | -&lt;br&gt; <br /> | -&lt;br&gt;<br /> |-<br /> | 2&lt;br&gt; <br /> | -&lt;br&gt; <br /> | Sigma&lt;br&gt; <br /> | PolyExpress&lt;br&gt;<br /> |-<br /> | 3&lt;br&gt; <br /> | TurboGFP&lt;br&gt; <br /> | Sigma&lt;br&gt; <br /> | PolyExpress&lt;br&gt;<br /> |-<br /> | 4&lt;br&gt; <br /> | TurboGFP&lt;br&gt; <br /> | 1:1&lt;br&gt; <br /> | PolyExpress&lt;br&gt;<br /> |-<br /> | 5&lt;br&gt; <br /> | TurboGFP&lt;br&gt; <br /> | 1:3&lt;br&gt; <br /> | PolyExpress&lt;br&gt;<br /> |-<br /> | 6&lt;br&gt; <br /> | TurboGFP&lt;br&gt; <br /> | 1:5&lt;br&gt; <br /> | PolyExpress&lt;br&gt;<br /> |-<br /> | 7&lt;br&gt; <br /> | TurboGFP&lt;br&gt; <br /> | 1:10&lt;br&gt; <br /> | PolyExpress&lt;br&gt;<br /> |-<br /> | 8&lt;br&gt; <br /> | TurboGFP&lt;br&gt; <br /> | Sigma&lt;br&gt; <br /> | FuGene&lt;br&gt;<br /> |-<br /> | 9&lt;br&gt; <br /> | TurboGFP&lt;br&gt; <br /> | 1:3&lt;br&gt; <br /> | FuGene&lt;br&gt;<br /> |-<br /> | 10&lt;br&gt; <br /> | pSMPUW-EGFP&lt;br&gt; <br /> | 1:3 <br /> | PolyExpress&lt;br&gt;<br /> |-<br /> | 11&lt;br&gt; <br /> | pSMPUW-EGFP&lt;br&gt; <br /> | Sigma&lt;br&gt; <br /> | PolyExpress&lt;br&gt;<br /> |-<br /> | 12&lt;br&gt; <br /> | pSMPUW-EGFP&lt;br&gt; <br /> | Sigma&lt;br&gt; <br /> | FuGene&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> Fugene: 1.2 ul&lt;br&gt; <br /> <br /> PolyExpress: 1.2 ul&lt;br&gt; <br /> <br /> Sigma TurboGFP:0.2 ug (1ul of 200 ng/ul)&lt;br&gt; <br /> <br /> pSMPUW-EGFP: 0.2 ug (1ul of 200 ng/ul)&lt;br&gt; <br /> <br /> Packaging mix: 2 ul (Sigma or homemade, pVSVG/pGag-Pol) <br /> <br /> &lt;br&gt; --[[User:Paul.xu|Paul.xu]] 16:49, 28 January 2011 (EST) <br /> <br /> [[Image:Packagingmix optimization 2 day1 post infection.JPG]] <br /> <br /> 1 day post infection <br /> <br /> &lt;br&gt; --[[User:Paul.xu|Paul.xu]] 11:01, 30 January 2011 (EST) <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:Lentivirus packaging mix optimization 2 infection day2.JPG]] <br /> <br /> 2 days after infection &lt;br&gt; <br /> <br /> [[Image:Lentivirus packaging mix optimization 2 infection day3.JPG]]&lt;br&gt; <br /> <br /> 3 days after infection &lt;br&gt; --[[User:Paul.xu|Paul.xu]] 10:38, 3 February 2011 (EST) <br /> <br /> [[Image:Lentivirus_packaging_mix_optimization_2_infection_day4.JPG]]&lt;br&gt;<br /> <br /> 4 days after infection &lt;br&gt;<br /> <br /> [[Image:Lentivirus_packaging_mix_optimization_2_infection_day5.JPG]]<br /> <br /> 5 days after infection &lt;br&gt;<br /> --[[User:Paul.xu|Paul.xu]] 14:23, 5 February 2011 (EST)<br /> &lt;br&gt;<br /> <br /> [[Category:Lentivirus]]</div> Paul.xu