Paul.xu at 15:32, 6 February 2011

2011-02-06T15:32:06Z

New page

Notebook #: 

Page #: 001431

2 days after transfection

[[Image:Packagingmix optimization 3 day2 after transfection.JPG]] 

 

[[Image:Lentivirus packaging mix optimizationg 3 day2.JPG]] --[[User:Paul.xu|Paul.xu]] 10:56, 30 January 2011 (EST) 

 

 

[[Image:Lentivirus packaging mix optimization 3 days 3.JPG]] 

3 days after transfection 

[[Image:Lentivirus packaging mix optimization 3 day4 .JPG]]

4 days after transfection

--[[User:Ztao|Ztao]] 10:10, 3 February 2011 (EST)

 

[[Image:Lentivirus packaging mix optimization 3 day5.JPG]] 

5 days after transfection

 --[[User:Paul.xu|Paul.xu]] 09:42, 4 February 2011 (EST)

 [[Image:Lentivirus packaging mix optimization 3 infection day1.JPG]] 

 

1 day after infection

 --Paul.xu 14:15, 1 February 2011 (EST) 

[[Image:Lentivirus packaging mix 2optimization 3 two days after infection.JPG]] 

2 days after infection --[[User:Ztao|Ztao]] 10:16, 3 February 2011 (EST) 

 

[[Image:Lentivirus packaging mix optimization 3 three days after infection.JPG]]

 

3 days after infection

--[[User:Paul.xu|Paul.xu]] 09:48, 4 February 2011 (EST)

[[Image:Lentivirus packaging mix optimization 3 four days after infection.JPG]] 4 days after infection --[[User:Paul.xu|Paul.xu]] 14:34, 5 February 2011 (EST)

 

[[Image:Lentivirus_packagingmix_optimization_3_five_days_after_infection.JPG]] 

5 days after infection 

--[[User:Paul.xu|Paul.xu]] 10:32, 6 February 2011 (EST)

{| cellspacing="1" cellpadding="1" border="1" style="width: 609px; height: 242px;"
|-
| 
| Vector 
| Packaging mix 
| Transfection Reagent 
|-
| 1 
| - 
| - 
| - 
|-
| 2 
| - 
| Sigma 
| PolyExpress 
|-
| 3 
| TurboGFP 
| Sigma 
| PolyExpress 
|-
| 4 
| TurboGFP 
| 1:1 
| PolyExpress 
|-
| 5 
| TurboGFP 
| 1:3 
| PolyExpress 
|-
| 6 
| TurboGFP 
| 1:5 
| PolyExpress 
|-
| 7 
| TurboGFP 
| 1:10 
| PolyExpress 
|-
| 8 
| TurboGFP 
| Sigma 
| GeneExpresso 
|-
| 9 
| TurboGFP 
| 1:3 
| GeneExpresso 
|-
| 10 
| pSMPUW-EGFP 
| 1:3 
| PolyExpress 
|-
| 11 
| pSMPUW-EGFP 
| Sigma 
| PolyExpress
|-
| 12 
| pSMPUW-EGFP 
| Sigma 
| GeneExpresso 
|}

 

GeneExpress: 1.2 ul/50ul 

PolyExpress: 1.2 ul/50ul 

TurboGFP:0.2 ug (1ul of 200 ng/ul) 

pSMPUW-EGFP: 0.2 ug (1ul of 200 ng/ul) 

Packaging mix: 2 ul (Sigma or homemade, pVSVG/pGag-Pol)

--[[User:Ztao|Ztao]] 17:00, 28 January 2011 (EST)

[[Category:Lentivirus]]

Packagingmix optimization 3 - Revision history

Paul.xu at 15:32, 6 February 2011