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Phusion purification 423 Jun 2012 - Revision history
2026-10-18T11:55:24Z
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Paul.xu at 17:33, 27 June 2012
2012-06-27T17:33:46Z
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<p><b>New page</b></p><div>== phusion purification from 423-pET30b-DeepVent-sso7d<br> ==<br />
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Date: 2012/6<br> <br />
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Experimenter: yingying lu <br />
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[[Image:Phusion NiCO21 6-6-2012.JPG]]<br> <br />
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2012/6/6 PCR<br> <br />
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2---12 elution tubes <br> <br />
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P:positive control phusion from FINNZYMES<br> <br />
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[[Image:Phusion Rosette 6-6-2012.JPG]]<br> <br />
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PCR test <br />
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2--9 elution tubes<br> <br />
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10:positive control <br> <br />
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[[Image:Phusion after desalt 6-8-2012.JPG]]<br> <br />
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2012/6/8 <br />
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PCR test <br />
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after desault with buffer C <br />
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[[Image:Phusion after PFLC 6-12-2012.JPG]] <br />
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2012/6/12 <br />
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PCR test&nbsp; after purification with HiTrap column <br />
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2012/6/13<br> <br />
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[[Image:Phusion purification1 6.13.2012.jpg]]<br> <br />
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1 1203<br> <br />
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2 1186<br> <br />
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3 1185<br> <br />
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4 Phusion<br> <br />
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5 Rosette (Purified)<br> <br />
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6 Nico (Purified)<br> <br />
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7 Rosette (FT) <br />
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8 Nico (FT)<br> <br />
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2012-6-27<br />
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PCR test phusion enzyme activity<br />
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[[Image:Phusion_enzyme_test_6-27-2012.JPG]]<br />
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[[Category:Protein_Purification]]</div>
Paul.xu