https://eln.excellgen.com/index.php?action=history&feed=atom&title=Plasmid_Purification_10232012 2026-10-18T07:05:42Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Plasmid_Purification_10232012&diff=6687&oldid=prev 2012-10-23T18:44:07Z

<p></p> <p><b>New page</b></p><div>Plasmid MaxiPrep Q Membrane using Mustang Q menbrance. &lt;br&gt;<br /> <br /> &lt;br&gt;<br /> <br /> 10/23/2012 <br /> <br /> Using protocol: Plasmid MaxiPrep Q Membrane&lt;br&gt;<br /> <br /> Sample : 1382, 260ml,&amp;nbsp; OD=2.04<br /> <br /> Adding 10 ml P1 (with RNase) +10ml P2+10ml P3, following the protocol except washing buffer B (0.75 M NaCl).<br /> <br /> Elute with buffer C (1.6M NaCl), collect 1ml fraction 1, 5 ml fraction 2 and 4 ml fraction 3. measure DNA using nanodrop.<br /> <br /> Fraction 1= 3ug/ml: fraction 2= 48.7ug/ml, fraction 3=10 ug/ml<br /> <br /> combine fraction 2 and 3 (280 ug DNA)&amp;nbsp; and precipitate with 0.7 Volume isopropanal. <br /> <br /> Centrifuge at 15000 rpm for 25 mins. <br /> <br /> Can see a&amp;nbsp; little white, glassy pellet, wash with 70% ethenal, dissove in 300 ul TE buffer. The conc. is 100ng/ul.&amp;nbsp; totally get 30ug DNA, DNA recovery is very low, about 10-15 % after precipitation.<br /> <br /> need to dissole in less volume to get high Conc. for next step transfection.<br /> <br /> [[Category:QC]]</div>

Paul.xu