https://eln.excellgen.com/index.php?action=history&feed=atom&title=PlentiGP-LoxP PlentiGP-LoxP - Revision history 2026-10-18T06:20:14Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=PlentiGP-LoxP&diff=1210&oldid=prev Ruili.su at 19:56, 28 June 2011 2011-06-28T19:56:20Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of LoxP to pLentiGP&#039;&#039;&#039; ==<br /> <br /> Purpose: cloning of LoxP to pLentiGP &lt;br&gt; <br /> <br /> Date: 2011-6-28 to 2011-6-29 <br /> <br /> Notebook:Ruili Su <br /> <br /> Page:002788--002789 <br /> <br /> Clone ID:# 259&lt;br&gt; <br /> <br /> Experimenters:&amp;nbsp; Ruili&lt;br&gt; <br /> <br /> &#039;&#039;&#039;PCR&#039;&#039;&#039;&lt;br&gt; <br /> <br /> Date: 2011-6-28 <br /> <br /> PCR to amplify LoxP <br /> <br /> [[Image:PlentiGP--LoxP-06-28-11-1.JPG]] <br /> <br /> 1. Marker <br /> <br /> 2. Amplified LoxP fregment from PCR. <br /> <br /> &#039;&#039;&#039;Subcloning&#039;&#039;&#039; <br /> <br /> Digest PlentiGP (clone 219) with BamH1+ XHoI&lt;br&gt; <br /> <br /> Digest LoxP fregment with BamH1+ XHoI&lt;br&gt; <br /> <br /> cut out insert and vector, ligate and transform STBL3 &lt;br&gt; <br /> <br /> [[Image:PlentiGP--LoxP-06-28-11-2.JPG]]&lt;br&gt; <br /> <br /> 1. Marker.&lt;br&gt; <br /> <br /> 2.&amp;nbsp;Digested PlentiGP (clone 219) with BamH1+ XHoI.&lt;br&gt; <br /> <br /> 3. Digested LoxP fregment with BamH1+ XHoI.&lt;br&gt; <br /> <br /> &lt;br&gt; &lt;br&gt; &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; <br /> <br /> &amp;nbsp;&amp;nbsp; &lt;br&gt; <br /> <br /> &#039;&#039;&#039;Conclusion&#039;&#039;&#039; <br /> <br /> --[[User:Ruili.su|Ruili.su]] 14:37, 28 June 2011 (EST) <br /> <br /> [[Category:Cloning]]</div> Ruili.su