Paul.xu: Created page with "2X Precipitation Solution: 40% (W/V) PEG 8000, 20 mM NaCl2 To DNA solution add PEG concentration of 13% in the presence of 10 mM MgCl2 at room tempera..."

2017-06-25T00:52:58Z

Created page with "2X Precipitation Solution: 40% (W/V) PEG 8000, 20 mM NaCl2 To DNA solution add PEG concentration of 13% in the presence of 10 mM MgCl2 at room tempera..."

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2X Precipitation Solution:  40% (W/V) PEG 8000, 20 mM NaCl2

To DNA solution add 

 PEG concentration of 13% in the presence of 10 mM MgCl2 at room temperature (20-22°C).

  5-10% (w/v) PEG 8000 ,  10 mM MgCl2 at room temperature

 PEG cannot be used for precipitation of DNA fragments less than 150 bp in size, the reagent is ideally suited for removal of low-molecularweight DNA fragments such as those obtained on double digestion with restriction enzymes.

Washing twice with 70% ethanol by centrifugation for 5 min at room temperature was found to effectively remove the leftover PEG
[[Category:Protocols]]

Precipitate DNA with PEG - Revision history

Paul.xu: Created page with "2X Precipitation Solution: 40% (W/V) PEG 8000, 20 mM NaCl2 To DNA solution add PEG concentration of 13% in the presence of 10 mM MgCl2 at room tempera..."