Paul.xu: Created page with "Dissolve 0.25 g Chitosan in 25 ml of 72 mM sulfuric acid solution (concentrated: 18.4 M, add 98~100 ul of Concentrated H₂SO₄ to 25 ml H2O-->72 mM) ..."

2014-07-21T21:54:06Z

Created page with "Dissolve 0.25 g Chitosan in 25 ml of 72 mM sulfuric acid solution (concentrated: 18.4 M, add 98~100 ul of Concentrated H2SO4 to 25 ml H2O-->72 mM) ..."

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Dissolve 0.25 g Chitosan in  25 ml of 72 mM sulfuric acid solution (concentrated: 18.4 M, add 98~100 ul of Concentrated H2SO4 to 25 ml H2O-->72 mM) in a 250 ml glass bottle 

Autoclave for 30 min.

Cool down on ice 

Adjust pH to 7~8 using 30 mL of 150 mM NaOH. 

Transfer to a centtrifuge tube, spin, collect pellet. Wash with water until pH is neutral 

 

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Preparation of Low MW Chitosan - Revision history

Paul.xu: Created page with "Dissolve 0.25 g Chitosan in 25 ml of 72 mM sulfuric acid solution (concentrated: 18.4 M, add 98~100 ul of Concentrated H2SO4 to 25 ml H2O-->72 mM) ..."

Paul.xu: Created page with "Dissolve 0.25 g Chitosan in 25 ml of 72 mM sulfuric acid solution (concentrated: 18.4 M, add 98~100 ul of Concentrated H₂SO₄ to 25 ml H2O-->72 mM) ..."