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Primery Amine Assay Copper - Revision history
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Paul.xu at 17:21, 4 December 2016
2016-12-04T17:21:55Z
<p></p>
<p><b>New page</b></p><div>Primary Amine Assay using CuCl<sub><sup>2</sup></sub><br />
<br />
1 M CuCl2: 5 ul<br />
<br />
PEI Solution: 10% (w/v) of PEI 1800, take 50 ul and diluted to 1000 ul,---> 0.5% (w/v), 0.5 g/100 ml<br />
<br />
0.05g/10 ml, 0.005g/1ml, 5 mg/1ml, 5 ug/ul&nbsp;<br />
<br />
<br />
<br />
{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| H2O, ul<br />
| PEI Std, ul<br />
| Amount<br />
| OD650 after 10 min<br />
| OD650 after 30 min<br />
|-<br />
| 95<br />
| 5<br />
| 25 ug<br />
| 0.071<br/><br />
| 0.173<br/><br />
|-<br />
| 90<br />
| 10<br />
| 50 ug<br />
| &nbsp;0.161<br/><br />
| 0.25<br/><br />
|-<br />
| 80<br />
| 20<br />
| 100 ug<br />
| &nbsp;0.386<br/><br />
| &nbsp;0.47<br/><br />
|-<br />
| 70<br />
| 30<br />
| 150 ug<br />
| &nbsp;0.508<br/><br />
| 0.585&nbsp;<br/><br />
|-<br />
| 60<br />
| 40<br />
| 200 ug<br />
| &nbsp;0.62&nbsp;<br/><br />
| 0.725<br/><br />
|-<br />
| 50<br />
| 50<br />
| 250 ug<br />
| 0.742<br/><br />
| 0.831<br/><br />
|-<br />
| 40<br />
| 60<br />
| 300 ug<br />
| 0.881<br/><br />
| 0.966<br/><br />
|-<br />
| 30<br />
| 70<br />
| 350 ug<br />
| &nbsp;0.995<br/><br />
| 1.059&nbsp;<br/><br />
|-<br />
| 20<br />
| 80<br />
| 400 ug<br />
| 1.143<br/><br />
| 1.212<br/><br />
|-<br />
| 10<br />
| 90<br />
| 450 ug<br />
| 1.265<br/><br />
| 1.339<br/><br />
|-<br />
| 5<br />
| 95<br />
| 475 ug<br />
| 1.357<br/><br />
| 1.406&nbsp;<br/><br />
|}<br />
<br />
PHP-DSP-Pyrozale: 5 ul 0.409; 10 ul: 0.653<br />
<br />
&nbsp;[[File:Primary amine assay.png|RTENOTITLE]]<br />
[[Category:Transfection]]</div>
Paul.xu