https://eln.excellgen.com/index.php?action=history&feed=atom&title=Proteases
Proteases - Revision history
2026-10-18T05:36:29Z
Revision history for this page on the wiki
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https://eln.excellgen.com/index.php?title=Proteases&diff=15734&oldid=prev
Paul.xu: Created page with "{| |- | 6His-3C | 0 | width="95" | 1797 | 1510 | pCold-6His-3C-MBP | 6His tagged wildtype native 3C fused to MBP, MBP at C-terminus | N-6His, C-MBP | width="49" | PCR | wt | 6..."
2017-04-07T14:04:01Z
<p>Created page with "{| |- | 6His-3C | 0 | width="95" | 1797 | 1510 | pCold-6His-3C-MBP | 6His tagged wildtype native 3C fused to MBP, MBP at C-terminus | N-6His, C-MBP | width="49" | PCR | wt | 6..."</p>
<p><b>New page</b></p><div>{|<br />
|-<br />
| 6His-3C<br />
| 0<br />
| width="95" | 1797<br />
| 1510<br />
| pCold-6His-3C-MBP<br />
| 6His tagged wildtype native 3C fused to MBP, MBP at C-terminus<br />
| N-6His, C-MBP<br />
| width="49" | PCR<br />
| wt<br />
| 6.01 / 62.6<br />
| width="227" | 6XHis-3C with small amount truncted 6XHis-3C-MBP fusion<br />
|-<br />
| 6His-GST-3C<br />
| 0<br />
| 1613<br />
| 1465<br />
| pET28-cold-GST-3C<br />
| native 3C fused to 6X His-GST<br />
| N-His-GST<br />
| PCR<br />
| wt<br />
| 6.99/47.4<br />
| full length<br />
|-<br />
| 6His-3C<br />
| 0<br />
| 1729<br />
| 1697<br />
| pCold-TF-(E3C)-3C<br />
| TF fused to autocleavable 3C protease<br />
| N-His-TF<br />
| sub<br />
| wt<br />
| 8.47 / 21.4<br />
| full length, but TF has His tag too<br />
|-<br />
| 6His-3C<br />
| 0<br />
| 1697<br />
| 1240<br />
| pCold-GST-(3c)-wt3C<br />
| autocleavage wild type 3C protease, fused to GST tag<br />
| N-His, GST at N, but autocleable<br />
| PCR<br />
| wt<br />
| 8.48/21.4<br />
| full length<br />
|-<br />
| 6His-3C<br />
| 0<br />
| 1606<br />
| 1510<br />
| pET28b-Cold-3Cwt<br />
| wild-type 3C<br />
| N-6His<br />
| Sub<br />
| wt<br />
| 8.76 / 21.6<br />
| -<br />
|-<br />
| 6XHis-GST-3C<br />
| 0<br />
| 1601<br />
| 1554<br />
| pT7A3-6His-GST-i3C<br />
| improved native HRV 3C<br />
| N-6XHis-GST, C-XHis<br />
| sub<br />
| my algorithm<br />
| 6.41 / 50.8<br />
| high<br />
|-<br />
| 6His-MBP-3C<br />
| 0<br />
| 1554<br />
| pT7A3-MBP-i3C<br />
| improved native HRV 3C<br />
| N-6XHis-MBP, C-6XHis<br />
| GS<br />
| my algorithm<br />
| 5.86 / 65.4<br />
| No, only MBP expressed<br />
|-<br />
| 6His-MBP-3C<br />
| 0<br />
| 1511<br />
| 1197<br />
| pT7A3-MBP.Synthetic-3C<br />
| E coli version of 3C<br />
| N-6XHis-MBP<br />
| sub<br />
| Invitrogen<br />
| 5.81 / 63.9<br />
| no<br />
|-<br />
| 6His-MBP-3C<br />
| 0<br />
| 1510<br />
| 1240<br />
| pT7A3-MBP.Native-3C<br />
| wt 3C fused to 6XHis-MBP<br />
| N-6XHis-MBP<br />
| sub<br />
| wt<br />
| 5.77 / 63.6<br />
| no<br />
|-<br />
| 6His-MBP-3C<br />
| 0<br />
| 1420<br />
| pDB.His.MBP.3C<br />
| wt 3C, N-6His_MBP<br />
| N-6XHis-MBP<br />
| GS<br />
| wt<br />
| 5.93 / 64.2<br />
| -<br />
|-<br />
| 6His-3C<br />
| 0<br />
| 1240<br />
| pET28b-3C<br />
| invitrogen E coli<br />
| N-6XHis<br />
| GS<br />
| Invitrogen<br />
| 8.76 / 22.6<br />
| -<br />
|-<br />
| 6His-GST-3C<br />
| 0<br />
| 1197<br />
| pDB.His.GST-3C<br />
| invitrogen E coli<br />
| N-6His-GST<br />
| GS<br />
| Invitrogen<br />
| 6.39 / 49.0<br />
| -<br />
|-<br />
| MBP-3C-6His<br />
| 0<br />
| 2092<br />
| 1601<br />
| pT7A3-MBP-i3C-6H<br />
| improved wt 3C fused to N-MBP and C-6His, no N-his tag<br />
| N-MBP, C-6His<br />
| PCR<br />
| my algorithm<br />
| 5.63 / 64.9<br />
| need to scren<br />
|}<br />
[[Category:Protein Purification]]</div>
Paul.xu