Paul.xu: Created page with "Relative binding strengths of antibodies from various species to protein G and protein A as measured in a competitive ELISA test. The amount of IgG required to give a 50% inhi..."

2018-08-10T18:59:29Z

Created page with "Relative binding strengths of antibodies from various species to protein G and protein A as measured in a competitive ELISA test. The amount of IgG required to give a 50% inhi..."

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Relative binding strengths of antibodies from various species to protein G and protein A as measured in a competitive ELISA test. The amount of IgG required to give a 50% inhibition of binding of rabbit IgG conjugated with alkaline phosphatase was determined.

{|
|-
! Species
! Subclass
! Protein G binding
! Protein A binding
|-
| Human
| IgA IgD IgE IgG1 IgG2 IgG3 IgG4 IgM*
| – – – ++++ ++++ ++++ ++++ –
| variable – – ++++ ++++ – ++++ variable
|-
| Avian egg yolk
| IgY†
| –
| –
|-
| Cow
|  
| ++++
| ++
|-
| Dog
|  
| +
| ++
|-
| Goat
|  
| ++
| –
|-
| Guinea pig
| IgG1
| ++
| ++++
|-
| Hamster
|  
| ++
| +
|-
| Horse
|  
| ++++
| ++
|-
| Koala
|  
| +
| –
|-
| Llama
|  
| +
| –
|-
| Monkey (rhesus)
|  
| ++++
| ++++
|-
| Mouse
| IgG1 IgG2a IgG2 IgG3 IgM*
| ++++ ++++ +++ +++ –
| + ++++ +++ ++ variable
|-
| Pig
|  
| +++
| +++
|-
| Rabbit
|  
| +++
| ++++
|-
| Rat
| IgG1 IgG2a IgG2 IgG3
| + ++++ ++ ++
| – – – +
|-
| Sheep
|  
| ++
| +/–
|}

* Puriﬁed using HiTrap IgM Puriﬁcation HP columns. † Puriﬁed using HiTrap IgY Puriﬁcation HP columns. ++++ = strong binding. ++ = medium binding. — = weak or no binding.
[[Category:Antibodies]]

Protein A G Binding Affinity - Revision history

Paul.xu: Created page with "Relative binding strengths of antibodies from various species to protein G and protein A as measured in a competitive ELISA test. The amount of IgG required to give a 50% inhi..."