https://eln.excellgen.com/index.php?action=history&feed=atom&title=Protein_Expression_8_17_2018 Protein Expression 8 17 2018 - Revision history 2026-10-18T10:20:13Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Protein_Expression_8_17_2018&diff=19076&oldid=prev Crm at 19:44, 20 August 2018 2018-08-20T19:44:30Z <p></p> <p><b>New page</b></p><div>Condition:0.25mM IPTG, RT, O/N<br /> <br /> [[File:Img019.jpg|RTENOTITLE]]<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | Lane<br /> | #<br /> | Name<br /> | Type<br /> | Pellet<br /> | MW(KD)<br /> | Note<br /> | Expression level<br /> |-<br /> | 1<br /> | 3122<br /> | pET30b-CRM197&lt;br/&gt;<br /> | S<br /> | loose<br /> | 63.1<br /> | Y&amp;nbsp;<br /> | very low<br /> |-<br /> | 2<br /> | 3127<br /> | pET28b-qBeta-qBeta-6XHis&lt;br/&gt;<br /> | S<br /> | loose<br /> | 15.6<br /> | Y<br /> | very low<br /> |-<br /> | M<br /> | Spetra BR protein standard<br /> | 5,10,25,35,40(red),50(green),70(brown),100,140(purple),260&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 3<br /> | 984<br /> | pT7A3-6H-SBP-SUMO-3C&lt;br/&gt;<br /> | S<br /> | loose<br /> | 27.7<br /> | N<br /> | &lt;br/&gt;<br /> |-<br /> | 4<br /> | 3248<br /> | pQE-80L-T7-RNAP-P266L&lt;br/&gt;<br /> | S<br /> | loose<br /> | 99.7<br /> | Y<br /> | high<br /> |-<br /> | 5<br /> | 129<br /> | pT7A30-TthMuts&lt;br/&gt;<br /> | S<br /> | loose<br /> | 91.8<br /> | N<br /> | &lt;br/&gt;<br /> |-<br /> | 6<br /> | 3122<br /> | &lt;br/&gt;<br /> | IB<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | N<br /> | &lt;br/&gt;<br /> |-<br /> | 7<br /> | 3127<br /> | &lt;br/&gt;<br /> | IB<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | Y<br /> | &lt;br/&gt;<br /> |-<br /> | 8<br /> | 984<br /> | &lt;br/&gt;<br /> | IB<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | N<br /> | &lt;br/&gt;<br /> |-<br /> | 9<br /> | 3248<br /> | &lt;br/&gt;<br /> | IB<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | N<br /> | &lt;br/&gt;<br /> |-<br /> | 10<br /> | 129<br /> | &lt;br/&gt;<br /> | IB<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | N<br /> | &lt;br/&gt;<br /> |-<br /> | 11<br /> | 1708<br /> | Cre<br /> | S<br /> | From protein purification<br /> | 43<br /> | Y<br /> | &lt;br/&gt;<br /> |}<br /> [[Category:Protein Expression]] [[Category:QC]]</div> Crm