https://eln.excellgen.com/index.php?action=history&feed=atom&title=Protein_Expression_Clone_Screening Protein Expression Clone Screening - Revision history 2026-10-18T02:13:48Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Protein_Expression_Clone_Screening&diff=9885&oldid=prev Paul.xu at 20:29, 31 July 2013 2013-07-31T20:29:48Z <p></p> <p><b>New page</b></p><div>== E coli Expression Screening 2012 ==<br /> <br /> sfGFP <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 750px;&quot;<br /> |-<br /> | Clone ID <br /> | Protein Name <br /> | Vector <br /> | Host <br /> | Condition <br /> | Expression Level <br /> | Solubility <br /> | Gel Image (Link) <br /> | Date <br /> | By<br /> |-<br /> | 360&lt;br&gt; <br /> | MBP&lt;br&gt; <br /> | pDB.His&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 3ml,37,0.2mMIPTG,5h&lt;br&gt; <br /> | high&lt;br&gt; <br /> | yes&lt;br&gt; <br /> | [[MBP,MMLV,UDG Protein Screening]]&lt;br&gt; <br /> | 11/7/2012&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1428]]&lt;br&gt; <br /> | MBP&lt;br&gt; <br /> | pT7&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 2ml,37,0.2mMIPTG,5h&lt;br&gt; <br /> | no&lt;br&gt; <br /> | no&lt;br&gt; <br /> | [[PT7-MBP-1428,pT7A3-MBP-1429,Nov,2,2012]]&lt;br&gt; <br /> | 11/2/2012&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1429-pT7A3-MBP|1429]]&lt;br&gt; <br /> | MBP&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 3ml,37,0.2mMIPTG,5h&lt;br&gt; <br /> | high&lt;br&gt; <br /> | yes&lt;br&gt; <br /> | <br /> [[MBP,MMLV,UDG Protein Screening]] <br /> <br /> [[PT7-MBP-1428,pT7A3-MBP-1429,Nov,2,2012]]&lt;br&gt; <br /> <br /> | <br /> 11/2/2012 <br /> <br /> 11/7/2012&lt;br&gt; <br /> <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[501]]&lt;br&gt; <br /> | MMLV&lt;br&gt; <br /> | PET30b&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 3ml,37,0.2mMIPTG,5h&lt;br&gt; <br /> | no <br /> | no&lt;br&gt; <br /> | [[MBP,MMLV,UDG Protein Screening]]&lt;br&gt; <br /> | 11/7/2012&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1034]]&lt;br&gt; <br /> | UDG&lt;br&gt; <br /> | PET8H&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 3ml,37,0.2mMIPTG,5h&lt;br&gt; <br /> | yes&lt;br&gt; <br /> | no&lt;br&gt; <br /> | [[MBP,MMLV,UDG Protein Screening]]&lt;br&gt; <br /> | 11/7/2012&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1375-pDB.His.MBP.3C|1375]]&lt;br&gt; <br /> | MBP-3C&lt;br&gt; <br /> | pDB-His&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 1L,37, 0.3mMIPTG,4h &lt;br&gt; <br /> | yes,only MBP, not 3C&lt;br&gt; <br /> | Yes&lt;br&gt; <br /> | [[PDB.His.MBP-3C]]&lt;br&gt; <br /> | 10/15/2012&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1308-pET-6H-Penetratin-Cre|1308]]&lt;br&gt; <br /> | CRE&lt;br&gt; <br /> | pET6H&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 2ml,37,0.5mMIPTG,4h &lt;br&gt; <br /> | Yes, but leaky, expressed in uninduced &lt;br&gt; <br /> | Yes&lt;br&gt; <br /> | [[PET -6H-Penetratin-NLS-Cre and pET -6H-VPMLK TAT NLS Cre]]&lt;br&gt; <br /> | &lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1311-pET-6H-VPMLK-TAT-NLS-Cre|1311]]&lt;br&gt; <br /> | CRE&lt;br&gt; <br /> | pET6H&lt;br&gt; <br /> | Nico21(DE3)plysN &lt;br&gt; <br /> | 2ml,37,0.5mMIPTG,4h &lt;br&gt; <br /> | High&lt;br&gt; <br /> | Yes&lt;br&gt; <br /> | [[PET -6H-Penetratin-NLS-Cre and pET -6H-VPMLK TAT NLS Cre]]&lt;br&gt; <br /> | &lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | 1307&lt;br&gt; <br /> | CRE&lt;br&gt; <br /> | pET6H&lt;br&gt; <br /> | Nico21(DE3)plysN &lt;br&gt; <br /> | 2ml,37,0.5mMIPTG,4h&lt;br&gt; <br /> | Faint, need recheck&lt;br&gt; <br /> | Yes&lt;br&gt; <br /> | [[1307-1309-1310 screening]]&lt;br&gt; <br /> | 11/7/2-12&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1310-pET-6H-VPMLK-NLS-Cre|1310]]&lt;br&gt; <br /> | CRE&lt;br&gt; <br /> | pET6H&lt;br&gt; <br /> | Nico21(DE3)plysN &lt;br&gt; <br /> | 2ml,37,0.5mMIPTG,4h&lt;br&gt; <br /> | High&lt;br&gt; <br /> | Yes&lt;br&gt; <br /> | [[1307-1309-1310 screening]]&lt;br&gt; <br /> | 11/7/2012&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1309-pET-6H-KLPVM-NLS-Cre|1309]]&lt;br&gt; <br /> | CRE&lt;br&gt; <br /> | pET6H&lt;br&gt; <br /> | Nico21(DE3)plysN &lt;br&gt; <br /> | 2ml,37,0.5mMIPTG,4h&lt;br&gt; <br /> | High&lt;br&gt; <br /> | Yes&lt;br&gt; <br /> | [[1307-1309-1310 screening]]&lt;br&gt; <br /> | 11/7/2012&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1143-1151-1186-screening|1143]]&lt;br&gt; <br /> | Exo-1&lt;br&gt; <br /> | pET8H&lt;br&gt; <br /> | Nico21(DE3)plysN &lt;br&gt; <br /> | 3 ml, 37, 0.5mM IPTG, O/N&lt;br&gt; <br /> | no&lt;br&gt; <br /> | no&lt;br&gt; <br /> | [[1143-1151-1186-screening]]&lt;br&gt; <br /> | 11/16/2012&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1143-1151-1186-screening|1151]]&lt;br&gt; <br /> | sso7d ToughTag, &lt;br&gt; <br /> | pET30b&lt;br&gt; <br /> | Nico21(DE3)plysN &lt;br&gt; <br /> | <br /> 3 ml, 37,0.5mM IPTG&lt;br&gt; <br /> <br /> O/N <br /> <br /> | no&lt;br&gt; <br /> | no&lt;br&gt; <br /> | [[1143-1151-1186-screening]]&lt;br&gt; <br /> | 11/16/2012&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1143-1151-1186-screening|1155]]&lt;br&gt; <br /> | Ara-S1&lt;br&gt; <br /> | pET28b&lt;br&gt; <br /> | Nico21(DE3)plysN &lt;br&gt; <br /> | <br /> 3 ml,37,0.5mM IPTG&lt;br&gt; <br /> <br /> O/N <br /> <br /> | no&lt;br&gt; <br /> | no&lt;br&gt; <br /> | [[1143-1151-1186-screening]]&lt;br&gt; <br /> | 11/16/2012&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1143-1151-1186-screening|1156]]&lt;br&gt; <br /> | Vent Exo&lt;br&gt; <br /> | p8H&lt;br&gt; <br /> | Nico21(DE3)plysN &lt;br&gt; <br /> | <br /> 3 ml, 37,0.5mM IPTG&lt;br&gt; <br /> <br /> O/N <br /> <br /> | Yes&lt;br&gt; <br /> | Yes&lt;br&gt; <br /> | [[1143-1151-1186-screening]]&lt;br&gt; <br /> | 11/16/2012&lt;br&gt;<br /> |-<br /> | [[1143-1151-1186-screening|1186]]&lt;br&gt; <br /> | MBP-Xis&lt;br&gt; <br /> | pDB.Xis&lt;br&gt; <br /> | Nico21(DE3)plysN &lt;br&gt; <br /> | <br /> 3 ml, 37,0.5mM IPTG, O/N <br /> <br /> | Yes&lt;br&gt; <br /> | Yes&lt;br&gt; <br /> | [[1143-1151-1186-screening]]&lt;br&gt; <br /> | 11/16/2012&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1140 1325 1225 1206 1203 1245 1249 1464|1140]]&lt;br&gt; <br /> | &lt;br&gt; polyAPol&lt;br&gt; <br /> | pTXB1&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | <br /> 3 ml, 37,0.5mM IPTG&lt;br&gt; <br /> <br /> 4 hs <br /> <br /> | No&lt;br&gt; <br /> | no&lt;br&gt; <br /> | [[1140 1325 1225 1206 1203 1245 1249 1464]]&lt;br&gt; <br /> | 11/30/2012&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1140 1325 1225 1206 1203 1245 1249 1464|1325]]&lt;br&gt; <br /> | MBP-gpI3L&lt;br&gt; <br /> | pDBHis&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | <br /> 3 ml,37,0.5mM IPTG&lt;br&gt; <br /> <br /> 4 hsYes <br /> <br /> | No&lt;br&gt; <br /> | no&lt;br&gt; <br /> | [[1140 1325 1225 1206 1203 1245 1249 1464]]&lt;br&gt; <br /> | 11/30/2012&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1140 1325 1225 1206 1203 1245 1249 1464|1225]]&lt;br&gt; <br /> | T7-Endo1&lt;br&gt; <br /> | pET28b&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | <br /> 3 ml, 37,0.5mM IPTG&lt;br&gt; <br /> <br /> O/N <br /> <br /> | No&lt;br&gt; <br /> | no&lt;br&gt; <br /> | [[1140 1325 1225 1206 1203 1245 1249 1464]]&lt;br&gt; <br /> | 11/30/2012&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1140 1325 1225 1206 1203 1245 1249 1464|1206]]&lt;br&gt; <br /> | recT&lt;br&gt; <br /> | pET28b&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | <br /> 3 ml,37,0.5mM IPTG, O/N <br /> <br /> | No&lt;br&gt; <br /> | no&lt;br&gt; <br /> | [[1140 1325 1225 1206 1203 1245 1249 1464]]&lt;br&gt; <br /> | 11/30/2012&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1140 1325 1225 1206 1203 1245 1249 1464|1203]]&lt;br&gt; <br /> | &lt;br&gt; scIHF2 &lt;br&gt; <br /> | pET42b&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | <br /> 3 ml, 37,0.5mM IPTG&lt;br&gt; <br /> <br /> O/N <br /> <br /> | No&lt;br&gt; <br /> | no&lt;br&gt; <br /> | [[1140 1325 1225 1206 1203 1245 1249 1464]]&lt;br&gt; <br /> | 11/30/2012&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1140 1325 1225 1206 1203 1245 1249 1464|1245]]&lt;br&gt; <br /> | &lt;br&gt; PfuMBP.scIHF2 &lt;br&gt; <br /> | pDBHIS&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | <br /> 3 ml, 37,0.5mM IPTG&lt;br&gt; <br /> <br /> O/N <br /> <br /> | No&lt;br&gt; <br /> | no&lt;br&gt; <br /> | [[1140 1325 1225 1206 1203 1245 1249 1464]]&lt;br&gt; <br /> | 11/30/2012&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1140 1325 1225 1206 1203 1245 1249 1464|1249]]&lt;br&gt; <br /> | &lt;br&gt; Taq_d289&lt;br&gt; <br /> | pET&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | <br /> 3 ml, 37,0.5mM IPTG&lt;br&gt; <br /> <br /> O/N <br /> <br /> | No&lt;br&gt; <br /> | no&lt;br&gt; <br /> | [[1140 1325 1225 1206 1203 1245 1249 1464]]&lt;br&gt; <br /> | 11/30/2012&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1140 1325 1225 1206 1203 1245 1249 1464|1464]]&lt;br&gt; <br /> | &lt;br&gt; T7 ligase&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | &lt;br&gt; <br /> | Yes&lt;br&gt; <br /> | Yes&lt;br&gt; <br /> | [[1140 1325 1225 1206 1203 1245 1249 1464]]&lt;br&gt; <br /> | 11/30/2012&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1137-1121-1291-1252-1206|1137]]&lt;br&gt; <br /> | Exo-VIII&lt;br&gt; <br /> | p8H&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | <br /> 3ml, 37,0.5mM IPTG&lt;br&gt; <br /> <br /> 4 hrs <br /> <br /> | No&lt;br&gt; <br /> | No&lt;br&gt; <br /> | [[1137-1121-1291-1252-1206]]&lt;br&gt; <br /> | 12/6/2012&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1137-1121-1291-1252-1206|1121]]&lt;br&gt; <br /> | Exo-1&lt;br&gt; <br /> | pET28b&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | <br /> 3ml,37,0.5mM IPTG&lt;br&gt; <br /> <br /> 4hrs <br /> <br /> | No&lt;br&gt; <br /> | No&lt;br&gt; <br /> | [[1137-1121-1291-1252-1206]] <br /> | 12/6/2012&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1137-1121-1291-1252-1206|1291]]&lt;br&gt; <br /> | TAT-NLS-sso7d&lt;br&gt; <br /> | pET30b&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | <br /> 3ml, 37,0.5mM IPTG&lt;br&gt; <br /> <br /> 4hrs <br /> <br /> | Yes&lt;br&gt; <br /> | Yes&lt;br&gt; <br /> | [[1137-1121-1291-1252-1206]]&lt;br&gt; <br /> | 12/6/2012&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1137-1121-1291-1252-1206|1252]]&lt;br&gt; <br /> | hLysozyme&lt;br&gt; <br /> | pTXB1&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | <br /> 3ml, 37,0.5mM IPTG&lt;br&gt; <br /> <br /> 4 hrs <br /> <br /> | Yes&lt;br&gt; <br /> | No&lt;br&gt; <br /> | [[1137-1121-1291-1252-1206]]&lt;br&gt; <br /> | 12/6/2012&lt;br&gt; <br /> | Xiuli&lt;br&gt;<br /> |-<br /> | [[1137-1121-1291-1252-1206|1206]]&lt;br&gt; <br /> | &lt;br&gt; recT&lt;br&gt; <br /> | pET28b&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | <br /> 3ml, 37,0.5mM IPTG <br /> <br /> 4 hrs <br /> <br /> | No&lt;br&gt; <br /> | No&lt;br&gt; <br /> | [[1137-1121-1291-1252-1206]]&lt;br&gt; <br /> | 12/6/2012&lt;br&gt; <br /> | <br /> Xiuli&lt;br&gt; <br /> <br /> |-<br /> | [[1437 pT7A3-RNaseA|1437]]&lt;br&gt; <br /> | RNAse A&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, 37,0.5mM IPTG <br /> O/N&lt;br&gt; <br /> <br /> | No&lt;br&gt; <br /> | No&lt;br&gt; <br /> | [[1437 1458 1494 1497 1498 1505 1508]]&lt;br&gt; <br /> | 12/31/2012&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1458 pT7A3-KOD|1458]]&lt;br&gt; <br /> | KOD&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, 37,0.5mM IPTG <br /> O/N&lt;br&gt; <br /> <br /> | No&lt;br&gt; <br /> | No&lt;br&gt; <br /> | [[1437 1458 1494 1497 1498 1505 1508]]&lt;br&gt; <br /> | 12/31/2012&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1494 pT7A3-UDG|1494]]&lt;br&gt; <br /> | UDG&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, 37,0.5mM IPTG <br /> O/N&lt;br&gt; <br /> <br /> | No&lt;br&gt; <br /> | No&lt;br&gt; <br /> | [[1437 1458 1494 1497 1498 1505 1508]]&lt;br&gt; <br /> | 12/31/2012&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1495-PT7A3-MMLV|1495]]&lt;br&gt; <br /> | MMLV&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 3ml, 37,0.5mM IPTG <br /> O/N&lt;br&gt; <br /> <br /> | No&lt;br&gt; <br /> | No&lt;br&gt; <br /> | [[1495-1496-1497-screening]]&lt;br&gt; <br /> | 12/20/2012&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1496-pT7A3-DNase1|1496]]&lt;br&gt; <br /> | Dnase1&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 3ml, 37,0.5mM IPTG <br /> O/N&lt;br&gt; <br /> <br /> | No&lt;br&gt; <br /> | No&lt;br&gt; <br /> | [[1495-1496-1497-screening]]&lt;br&gt; <br /> | 12/20/2012&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1497-pT7A3-ToughTaq|1497]]&lt;br&gt; <br /> | ToughTaq&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 3ml, 37,0.5mM IPTG <br /> O/N&lt;br&gt; <br /> <br /> | Yes&lt;br&gt; <br /> | Yes&lt;br&gt; <br /> | [[1495-1496-1497-screening]]&lt;br&gt; <br /> | 12/20/2012&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1498-pT7A3-TaqDNALigase|1498]]&lt;br&gt; <br /> | Tagligase&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, 37,0.5mM IPTG <br /> O/N&lt;br&gt; <br /> <br /> | Yes&lt;br&gt; <br /> | Yes&lt;br&gt; <br /> | [[1437 1458 1494 1497 1498 1505 1508]]&lt;br&gt; <br /> | 12/31/2012&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1505 pT7A3-T7-Endo1|1505]]&lt;br&gt; <br /> | T7-endo1&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, 37,0.5mM IPTG <br /> O/N&lt;br&gt; <br /> <br /> | No&lt;br&gt; <br /> | No&lt;br&gt; <br /> | [[1437 1458 1494 1497 1498 1505 1508]]&lt;br&gt; <br /> | 12/31/2012&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1508 pT7A3-DNA-Pol-1|1508]]&lt;br&gt; <br /> | DNA-pol-1&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | BL21(DE3)Star&lt;br&gt; <br /> | 5ml, 37,0.5mM IPTG <br /> O/N&lt;br&gt; <br /> <br /> | No&lt;br&gt; <br /> | No&lt;br&gt; <br /> | [[1437 1458 1494 1497 1498 1505 1508]]&lt;br&gt; <br /> | 12/31/2012&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1510 pT7A3-MBP.Native-3C|1510]]&lt;br&gt; <br /> | MBP-native 3C&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | &lt;br&gt; <br /> | 5ml, 37,0.5mM IPTG <br /> O/N&lt;br&gt; <br /> <br /> | &amp;nbsp;only MBP &lt;br&gt; <br /> | only MBP &lt;br&gt; <br /> | <br /> [[1465 1510 1511 1514 1517]] <br /> <br /> [[1510 1511]] <br /> <br /> &lt;br&gt; <br /> <br /> | <br /> 12/31/2012 <br /> <br /> 1/3/2013&lt;br&gt; <br /> <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1511 pT7A3-MBP.Synthetic-3C|1511]]&lt;br&gt; <br /> | MBP-synthetic 3C&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | &lt;br&gt; <br /> | 5ml, 37,0.5mM IPTG <br /> O/N&lt;br&gt; <br /> <br /> | only MBP &lt;br&gt; <br /> | only MBP &lt;br&gt; <br /> | <br /> [[1465 1510 1511 1514 1517]] <br /> <br /> [[1510 1511]] <br /> <br /> | <br /> 12/31/2012 <br /> <br /> 1/3/2013&lt;br&gt; <br /> <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1514 pT7A3-MBP-(3C)|1514]]&lt;br&gt; <br /> | MBP- 3C&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, 37,0.5mM IPTG <br /> O/N&lt;br&gt; <br /> <br /> | No&lt;br&gt; <br /> | No&lt;br&gt; <br /> | [[1465 1510 1511 1514 1517]]&lt;br&gt; <br /> | 12/31/2012&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1517 pT7A3-MBP-(3C)-sfGFP|1517]]&lt;br&gt; <br /> | MBP-sfGFP&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, 37,0.5mM IPTG <br /> O/N&lt;br&gt; <br /> <br /> | Yes&lt;br&gt; <br /> | Yes&lt;br&gt; <br /> | [[1465 1510 1511 1514 1517]]&lt;br&gt; <br /> | 12/31/2012&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1554-pT7A3-MBP-i3C|1554]]&lt;br&gt; <br /> | MBP-i3C&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | BL21DE3pRare2,BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, 25,0.3mM IPTG <br /> O/N&lt;br&gt; <br /> <br /> | only MBP &lt;br&gt; <br /> | only MBP &lt;br&gt; <br /> | [[1554 1613]]&lt;br&gt; <br /> | 2013/1/18&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1613-pET28-cold-GST-3C|1613]]&lt;br&gt; <br /> | GST-3C&lt;br&gt; <br /> | pET28b&lt;br&gt; <br /> | BL21DE3pRare2,BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, 25,0.3mM IPTG <br /> O/N&lt;br&gt; <br /> <br /> | Yes&lt;br&gt; <br /> | Yes&lt;br&gt; <br /> | [[1554 1613]]&lt;br&gt; <br /> | 2013/1/18&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1049 pT7A3-T4 RNA Ligase 1|1049 ]]&lt;br&gt; <br /> | T4_RNA_Ligase_1&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | <br /> 5ml,&lt;br&gt; <br /> <br /> 0.2 mM IPTG RT o/n <br /> <br /> | Yes&lt;br&gt; <br /> | Yes&lt;br&gt; <br /> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]&lt;br&gt; <br /> | 2013/3/11&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1123 pT7A3-MBP-(3C)-T4-RNA-Lig2|1123 ]]&lt;br&gt; <br /> | MBP-(3C)-T4-RNA-Lig2&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | Yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]&lt;br&gt; <br /> | 2013/3/11&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1501 pT7A3-Klenow|1501 ]]&lt;br&gt; <br /> | Klenow&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | no&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]&lt;br&gt; <br /> | 2013/3/11&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1503 pT7A3-T4 RNA Ligase 2 Truncated|1503 ]]&lt;br&gt; <br /> | T4_RNA_Ligase_2_Truncated&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]&lt;br&gt; <br /> | 2013/3/11&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1508 pT7A3-DNA-Pol-1|1508 ]]&lt;br&gt; <br /> | DNA-Pol-1&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]&lt;br&gt; <br /> | 2013/3/11&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1512 pT7A3-Phi29DNAPol|1512 ]]&lt;br&gt; <br /> | Phi29DNAPol&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]&lt;br&gt; <br /> | 2013/3/11&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1513-pT7A3-polyAPol|1513]]&lt;br&gt; <br /> | polyAPol&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | no&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]&lt;br&gt; <br /> | 2013/3/11&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1537 pT7A3-Klenow3&#039;-5&#039;exo–|1537]]&lt;br&gt; <br /> | Klenow3&#039;-5&#039;exo–&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | no&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]&lt;br&gt; <br /> | 2013/3/11&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1557 pT7A3-MBP-(3c)-Lambda-Exo|1557 ]]&lt;br&gt; <br /> | MBP-(3c)-Lambda-Exo&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | &amp;nbsp;?&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]&lt;br&gt; <br /> | 2013/3/11&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1572 pT7A3-MBP-(3c)-Endonuclease VIII|1572 ]]&lt;br&gt; <br /> | MBP-(3c)-Endonuclease_VIII&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1049 1123 1501 1503 1508 1512 1513 1537 1557 1572]]&lt;br&gt; <br /> | 2013/3/11&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1509-pT7A3-hTdT|1509]]&lt;br&gt; <br /> | hTdT&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]&lt;br&gt; <br /> | 2013/3/20&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1533 pET28b-T7-DNA-Polymerase|1533]]&lt;br&gt; <br /> | T7-DNA-Polymerase&lt;br&gt; <br /> | pET28b&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]&lt;br&gt; <br /> | 2013/3/20&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1539-pET28b-T7-DNA-Pol-D28|1539]]&lt;br&gt; <br /> | T7-DNA-Pol-D28human ribonuclease/angiogenin inhibitor 1 <br /> | pET28b&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]&lt;br&gt; <br /> | 2013/3/20&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1569 pT7A3-MBB-(3C)-Exo-III|1569]]&lt;br&gt; <br /> | MBP-(3C)-Exo-III&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]&lt;br&gt; <br /> | 2013/3/20&lt;br&gt;<br /> |-<br /> | [[1581 pT7A3-T4RNA-Lig2-truncated-KQ|1581]]&lt;br&gt; <br /> | T4RNA-Lig2-truncated-KQ&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]&lt;br&gt; <br /> | 2013/3/20&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1591 pT7A3-MBP-(3c)-Ecoli-UDG|1591]]&lt;br&gt; <br /> | MBP-(3c)-Ecoli-UDG&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]&lt;br&gt; <br /> | 2013/3/20&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1617 pT7A3-MBP-(3c)-newPhi29|1617]]&lt;br&gt; <br /> | MBP-(3c)-newPhi29&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | &amp;nbsp;?&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]&lt;br&gt; <br /> | 2013/3/20&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1570 pT7A3-MBP-(3C)-Exo-1|1570]]&lt;br&gt; <br /> | MBP-(3C)-Exo-1&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]&lt;br&gt; <br /> | 2013/3/20&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1501 pT7A3-Klenow|1501]]&lt;br&gt; <br /> | Klenow&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.1 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | &amp;nbsp;?&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]&lt;br&gt; <br /> | 2013/3/20&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1537 pT7A3-Klenow3&#039;-5&#039;exo–|1537]]&lt;br&gt; <br /> | Klenow3&#039;-5&#039;exo–&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.1 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]&lt;br&gt; <br /> | 2013/3/20&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1529 pT7A3-Ecoli RecA|1529]]&lt;br&gt; <br /> | Ecoli_RecA&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1509 1533 1539 1569 1581 1591 1617 1570 1501 1537 1529]]&lt;br&gt; <br /> | 2013/3/20&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1542-pT7A3-sso7d-Native-ToughTaq|1542]]&lt;br&gt; <br /> | Native-ToughTaq&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1542 1544 1545 1627 1679 1680]]&lt;br&gt; <br /> | 2013/3/28&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1543-pT7A3-VVCap-S|1544]]&lt;br&gt; <br /> | VVCap-L&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1542 1544 1545 1627 1679 1680]]&lt;br&gt; <br /> | 2013/3/28&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1545-pT7A3-VVCap|1545]]&lt;br&gt; <br /> | VVCap&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | stop inter&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1542 1544 1545 1627 1679 1680]]&lt;br&gt; <br /> | 2013/3/28&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1627-pT7A3-SOD|1627]]&lt;br&gt; <br /> | SOD&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1542 1544 1545 1627 1679 1680]]&lt;br&gt; <br /> | 2013/3/28&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1097-pET28b-T4-G32|1097]]&lt;br&gt; <br /> | T4-G32&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | NO&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]&lt;br&gt; <br /> | 2013/3/29&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1138 p8H-MutM|1138]]&lt;br&gt; <br /> | MutM&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]&lt;br&gt; <br /> | 2013/3/29&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1458 pT7A3-KOD|1458]]&lt;br&gt; <br /> | KOD&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]&lt;br&gt; <br /> | 2013/3/29&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1494 pT7A3-UDG|1494]]&lt;br&gt; <br /> | UDG&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | NO&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]&lt;br&gt; <br /> | 2013/3/29&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1505 pT7A3-T7-Endo1|1505]]&lt;br&gt; <br /> | T7-Endo1&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]&lt;br&gt; <br /> | 2013/3/29&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1513-pT7A3-polyAPol|1513]]&lt;br&gt; <br /> | polyAPol&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | NO&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]&lt;br&gt; <br /> | 2013/3/29&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1558 pT7A3-T7-Exo|1558]]&lt;br&gt; <br /> | T7-Exo&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | NO&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]&lt;br&gt; <br /> | 2013/3/29&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1562 pT7A3-T4-Endonuclease-VII|1562]]&lt;br&gt; <br /> | T4-Endonuclease-VII&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | NO&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]&lt;br&gt; <br /> | 2013/3/29&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1573-pT7A3-MBP-(3C)-RnaseH|1573]]&lt;br&gt; <br /> | MBP-(3C)-RnaseH&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]&lt;br&gt; <br /> | 2013/3/29&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1593 pT7A3-MBP-(3c)-E coli Endo IV|1593]]&lt;br&gt; <br /> | MBP-(3c)-E_coli_Endo_IV&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]&lt;br&gt; <br /> | 2013/3/29&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1654 pT7A3-T3 DNA ligase|1654]]&lt;br&gt; <br /> | T3_DNA_ligase&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1097 1138 1458 1494 1505 1513 1558 1562 1573 1593 1654]]&lt;br&gt; <br /> | 2013/3/29&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1567 pT7A3-T5-Exo|1567]]&lt;br&gt; <br /> | T5-Exo&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1696 1707 1567 1673 1697]]&lt;br&gt; <br /> | 2013/4/5&lt;br&gt; <br /> | Paul&lt;br&gt;<br /> |-<br /> | [[1614-pCold-TF-(3c)|1614]]&lt;br&gt; <br /> | TF&lt;br&gt; <br /> | pCold&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1567 1614 1673 1677 1692 1694 1696 1697 1707 1711]]&lt;br&gt; <br /> | 2013/3/30&lt;br&gt; <br /> | Paul&lt;br&gt;<br /> |-<br /> | [[1673-pET28b-NdeI|1673]]&lt;br&gt; <br /> | Nde1&lt;br&gt; <br /> | pET28b&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1696 1707 1567 1673 1697]]&lt;br&gt; <br /> | 2013/4/5&lt;br&gt; <br /> | Paul&lt;br&gt;<br /> |-<br /> | [[1677-pTCold-MBP-(3C)|1677]]&lt;br&gt; <br /> | MBP&lt;br&gt; <br /> | pTCold&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1567 1614 1673 1677 1692 1694 1696 1697 1707 1711]]&lt;br&gt; <br /> | 2013/3/30&lt;br&gt; <br /> | Paul&lt;br&gt;<br /> |-<br /> | [[1694-pT7A3-wtSM2|1692]]&lt;br&gt; <br /> | MBP&lt;br&gt; <br /> | pCold&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | Paul&lt;br&gt;<br /> |-<br /> | [[1694-pT7A3-wtSM2|1694]]&lt;br&gt; <br /> | wtSM2&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt; <br /> | 2013/3/30&lt;br&gt; <br /> | Paul&lt;br&gt;<br /> |-<br /> | [[1697-pCold-GST-(3c)-wt3C|1696]]&lt;br&gt; <br /> | MBP-(3C)-TEV&lt;br&gt; <br /> | pCold-&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1567 1614 1673 1677 1692 1694 1696 1697 1707 1711]]&lt;br&gt; <br /> | 2013/3/30&lt;br&gt; <br /> | Paul&lt;br&gt;<br /> |-<br /> | [[1697-pCold-GST-(3c)-wt3C|1697]]&lt;br&gt; <br /> | GST-(3c)-wt3C&lt;br&gt; <br /> | pCold-&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1696 1707 1567 1673 1697]]&lt;br&gt; <br /> | 2013/4/5&lt;br&gt; <br /> | Paul&lt;br&gt;<br /> |-<br /> | [[1707-pT7A3-MBP-(E3C)-p53wt|1707]]&lt;br&gt; <br /> | MBP-(E3C)-p53wt&lt;br&gt; <br /> | pT7A3-&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1567 1614 1673 1677 1692 1694 1696 1697 1707 1711]]&lt;br&gt; <br /> | 2013/3/30&lt;br&gt; <br /> | Paul&lt;br&gt;<br /> |-<br /> | [[623 pT7A3-8H-IL-4|623]]&lt;br&gt; <br /> | &lt;br&gt; pT7A3-8H-IL-4&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | &amp;nbsp;?&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]&lt;br&gt; <br /> | 2013/05/02&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1248 pT7A3-DeepVent-Exo|1248]]&lt;br&gt; <br /> | DeepVent-Exo&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | NO&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]&lt;br&gt; <br /> | 2013/05/02&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1285 pT7A3-Proteinase-K|1285]]&lt;br&gt; <br /> | Proteinase-K&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]&lt;br&gt; <br /> | 2013/05/02&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1313 pT7A3-RNAse 1|1313]]&lt;br&gt; <br /> | RNAse_1&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]&lt;br&gt; <br /> | 2013/05/02&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1314 pT7-RppH|1314]]&lt;br&gt; <br /> | RppH&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]&lt;br&gt; <br /> | 2013/05/02&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1317 pT7A3-RNH1|1317]]&lt;br&gt; <br /> | RNH1(human ribonuclease/angiogenin inhibitor 1)&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | NO&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]&lt;br&gt; <br /> | 2013/05/02&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1494 pT7A3-UDG|1494]]&lt;br&gt; <br /> | UDG&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | &amp;nbsp;?&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]&lt;br&gt; <br /> | 2013/05/02&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1536 pT7A3-RNH1|1536]]&lt;br&gt; <br /> | RNH1(Human RNase Inhibitor)&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | NO&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]&lt;br&gt; <br /> | 2013/05/02&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1705 pT7A3-ZB2-T4Lysozyme|1705]]&lt;br&gt; <br /> | ZB2-T4Lysozyme&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | NO&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]&lt;br&gt; <br /> | 2013/05/02&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1706 pT7A3-BirA|1706]]&lt;br&gt; <br /> | BirA &lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]&lt;br&gt; <br /> | 2013/05/02&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1732 pT7A3-Ecoli-Ligase|1732]]&lt;br&gt; <br /> | Ecoli-Ligase&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[623 1248 1285 1313 1314 1317 1494 1536 1705 1706 1732]]&lt;br&gt; <br /> | 2013/05/02&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | [[1437 pT7A3-RNaseA|1437]]&lt;br&gt; <br /> | RNaseA&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YES&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1437 1568]]&lt;br&gt; <br /> | 2013/05/02&lt;br&gt; <br /> | Shanyi&lt;br&gt;<br /> |-<br /> | <br /> [[1568 pT7A3-Exo-VIII|1568]] <br /> <br /> &lt;br&gt; <br /> <br /> | MBP-Exo-VIII&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | YSE&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1437 1568]]&lt;br&gt; <br /> | &lt;br&gt; <br /> | &lt;br&gt;<br /> |-<br /> | 1711&lt;br&gt; <br /> | hINFA2&lt;br&gt; <br /> | pET28b&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1458 1654 1711 1712]]&lt;br&gt; <br /> | 2013/4/12&lt;br&gt; <br /> | yingying<br /> |-<br /> | 1712&lt;br&gt; <br /> | hINFA2&lt;br&gt; <br /> | pLambda&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | 37 oc culture, 42 oC induction for 2h&lt;br&gt; <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1458 1654 1711 1712]]&lt;br&gt; <br /> | 2013/4/12&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[626-pT7A3-Taq d289|626]]&lt;br&gt; <br /> | Taq d289&lt;br&gt; <br /> | pT7A3&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | &amp;nbsp;?&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[626 1717 1719 1720]]&lt;br&gt; <br /> | 2013/5/2&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | <br /> [[1717-pT7A3-6H-(TEV)-MBP-(3C)|1717]]&lt;br&gt; <br /> <br /> | MBP&lt;br&gt; <br /> | pT7A3-MBP-(E3C)&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[626 1717 1719 1720]]&lt;br&gt; <br /> | 2013/5/2&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1719-pT7A3-MBP-(E3C)-NRF2|1719]]&lt;br&gt; <br /> | NRF2&lt;br&gt; <br /> | pT7A3-MBP-(E3C)&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | &amp;nbsp;?&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[626 1717 1719 1720]]&lt;br&gt; <br /> | 2013/5/2&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1720-pT7A3-MBP-(E3C)-Keap1|1720]]&lt;br&gt; <br /> | Keap1&lt;br&gt; <br /> | pT7A3-MBP-(E3C)&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | &amp;nbsp;?&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[626 1717 1719 1720]]&lt;br&gt; <br /> | 2013/5/2&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1729-pCold-TF-(E3C)-3C|1729]]&lt;br&gt; <br /> | TF-(E3C)-3C&lt;br&gt; <br /> | pCold-TF-(E3C)-3C&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1724 1729 1731 1741 1742 1743 1744 1745 1748 1749]]&lt;br&gt; <br /> | 2013/5/21&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1724-pT7A3-6His-sc-VVCap|1724]]&lt;br&gt; <br /> | sc-VVCap&lt;br&gt; <br /> | pT7A3-6His-sc-VVCap&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1724 1729 1731 1741 1742 1743 1744 1745 1748 1749]]&lt;br&gt; <br /> | 2013/5/21&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1741-pIC-sfGFP|1741]]&lt;br&gt; <br /> | sfGFP&lt;br&gt; <br /> | pIC-sfGFP&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1724 1729 1731 1741 1742 1743 1744 1745 1748 1749]]&lt;br&gt; <br /> | 2013/5/21&lt;br&gt; <br /> | yingying&lt;br&gt;<br /> |-<br /> | [[1745-p8HIC-sfGFP|1745]]&lt;br&gt; <br /> | sfGFP&lt;br&gt; <br /> | p8HIC-sfGFP&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[1724 1729 1731 1741 1742 1743 1744 1745 1748 1749]]&lt;br&gt; <br /> | 2013/5/21yingying&lt;br&gt;<br /> |-<br /> | [[643-pT7A3-Nhe1|643]]&lt;br&gt; <br /> | Nhe1&lt;br&gt; <br /> | pT7A3-Nhe1&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[643 645 652 1721 1731 1736 1757 1764 1771]]&lt;br&gt; <br /> | 2013/7/9yingying&lt;br&gt;<br /> |-<br /> | [[645-pT7A3-Pst1|645]]&lt;br&gt; <br /> | Pst1&lt;br&gt; <br /> | pT7A3-Pst1&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[643 645 652 1721 1731 1736 1757 1764 1771]]&lt;br&gt; <br /> | 2013/7/9yingying&lt;br&gt;<br /> |-<br /> | [[652-pT7A30-hIFNA2|652]]&lt;br&gt; <br /> | hIFNA2&lt;br&gt; <br /> | pT7A30-hIFNA2&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | weak&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[643 645 652 1721 1731 1736 1757 1764 1771]]&lt;br&gt; <br /> | 2013/7/9yingying&lt;br&gt;<br /> |-<br /> | [[1736-pT7A3-His-NusA|1736]]&lt;br&gt; <br /> | His-NusA&lt;br&gt; <br /> | pT7A3-His-NusA&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[643 645 652 1721 1731 1736 1757 1764 1771]]&lt;br&gt; <br /> | 2013/7/9yingying&lt;br&gt;<br /> |-<br /> | [[1764-pT7A3-T4-Pnk|1764]]&lt;br&gt; <br /> | T4-Pnk&lt;br&gt; <br /> | pT7A3-T4-Pnk&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[643 645 652 1721 1731 1736 1757 1764 1771]]&lt;br&gt; <br /> | 2013/7/9yingying&lt;br&gt;<br /> |-<br /> | [[1771-pT7A3-MBP-(3C)-PCK1|1771]]&lt;br&gt; <br /> | PCK1&lt;br&gt; <br /> | pT7A3-MBP-(3C)-PCK1&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | yes&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[643 645 652 1721 1731 1736 1757 1764 1771]]&lt;br&gt; <br /> | 2013/7/9yingying&lt;br&gt;<br /> |-<br /> | [[1721-pT7A3-MBP-(E3C)-SOD|1721]]&lt;br&gt; <br /> | MBP-(E3C)-SOD&lt;br&gt; <br /> | pT7A3-MBP-(E3C)-SOD&lt;br&gt; <br /> | Nico21(DE3)plysN&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | only MBP&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[643 645 652 1721 1731 1736 1757 1764 1771]]&lt;br&gt; <br /> | 2013/7/9yingying&lt;br&gt;<br /> |-<br /> | [[1731-pT7A3-TurboEnterokinase|1731]]&lt;br&gt; <br /> | TurboEnterokinase&lt;br&gt; <br /> | pT7A3-TurboEnterokinase&lt;br&gt; <br /> | BL21DE3pRare2pLysS&lt;br&gt; <br /> | 5ml, <br /> 0.2 mM IPTG RT o/n&lt;br&gt; <br /> <br /> | &amp;nbsp;?weak&lt;br&gt; <br /> | &lt;br&gt; <br /> | [[643 645 652 1721 1731 1736 1757 1764 1771]]&lt;br&gt; <br /> | 2013/7/9yingying&lt;br&gt;<br /> |-<br /> | [[631-pT7A3-IL-22|631]]&lt;br&gt;<br /> | IL-22&lt;br&gt;<br /> | pT7A3-IL-22&lt;br&gt;<br /> | NiCO21(DE3)pLysN&lt;br&gt;<br /> | 5ml,25C<br /> 0.2 mM IPTG RT o/n&lt;br&gt;<br /> <br /> | NO&lt;br&gt;<br /> | &lt;br&gt;<br /> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]&lt;br&gt;<br /> | <br /> 2013/7/30<br /> <br /> yingying&lt;br&gt;<br /> <br /> |-<br /> | [[1497-pT7A3-ToughTaq|1497]]&lt;br&gt;<br /> | ToughTaq&lt;br&gt;<br /> | pT7A3-ToughTaq&lt;br&gt;<br /> | BL21(DE3)Star, pRare2-pLysS &lt;br&gt;<br /> | 5ml,37C<br /> 0.2 mM IPTG RT o/n&lt;br&gt;<br /> <br /> | weak&lt;br&gt;<br /> | &lt;br&gt;<br /> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]&lt;br&gt;<br /> | 2013/7/30<br /> yingying&lt;br&gt;<br /> <br /> |-<br /> | [[1542-pT7A3-sso7d-Native-ToughTaq|1542]]&lt;br&gt;<br /> | sso7d-Native-ToughTaq&lt;br&gt;<br /> | pT7A3-sso7d-Native-ToughTaq&lt;br&gt;<br /> | BL21(DE3)Star, pRare2-pLysS &lt;br&gt;<br /> | 5ml,37C<br /> 0.2 mM IPTG RT o/n&lt;br&gt;<br /> <br /> | weak&lt;br&gt;<br /> | &lt;br&gt;<br /> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]&lt;br&gt;<br /> | 2013/7/30<br /> yingying&lt;br&gt;<br /> <br /> |-<br /> | [[1719-pT7A3-MBP-(E3C)-NRF2|1719]]&lt;br&gt;<br /> | MBP-(E3C)-NRF2&lt;br&gt;<br /> | pT7A3-MBP-(E3C)-NRF2&lt;br&gt;<br /> | BL21(DE3)Star, pRare2-pLysS &lt;br&gt;<br /> | 5ml,25C<br /> 0.2 mM IPTG RT o/n&lt;br&gt;<br /> <br /> | yes&lt;br&gt;<br /> | &lt;br&gt;<br /> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]&lt;br&gt;<br /> | 2013/7/30<br /> yingying&lt;br&gt;<br /> <br /> |-<br /> | [[1748-pCold-8His-MBP-NRF2-6His|1748]]&lt;br&gt;<br /> | -MBP-NRF2&lt;br&gt;<br /> | pCold-8His-MBP-NRF2-6His&lt;br&gt;<br /> | BL21(DE3)Star, pRare2-pLysS &lt;br&gt;<br /> | 5ml,25C<br /> 0.2 mM IPTG RT o/n&lt;br&gt;<br /> <br /> | yes&lt;br&gt;<br /> | &lt;br&gt;<br /> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]&lt;br&gt;<br /> | 2013/7/30<br /> yingying&lt;br&gt;<br /> <br /> |-<br /> | [[1765-pT7A3-T4DNAPol|1765]]&lt;br&gt;<br /> | T4DNAPol&lt;br&gt;<br /> | pT7A3-T4DNAPol&lt;br&gt;<br /> | Nico21(DE3)plysN&lt;br&gt;<br /> | 5ml,25C<br /> 0.2 mM IPTG RT o/n&lt;br&gt;<br /> <br /> | yes&lt;br&gt;<br /> | &lt;br&gt;<br /> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]&lt;br&gt;<br /> | 2013/7/30<br /> yingying&lt;br&gt;<br /> <br /> |-<br /> | [[1780-pT7A3-T7-RNAPol-Y639F|1780]]&lt;br&gt;<br /> | T7-RNAPol-Y639F&lt;br&gt;<br /> | pT7A3-T7-RNAPol-Y639F&lt;br&gt;<br /> | Nico21(DE3)plysN&lt;br&gt;<br /> | 5ml,25C<br /> 0.2 mM IPTG RT o/n&lt;br&gt;<br /> <br /> | no&lt;br&gt;<br /> | &lt;br&gt;<br /> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]&lt;br&gt;<br /> | 2013/7/30<br /> yingying&lt;br&gt;<br /> <br /> |-<br /> | [[1781-pTCold-MBP-(3C-TEV)-PKARIIb|1781]]&lt;br&gt;<br /> | MBP-(3C-TEV)-PKARIIb&lt;br&gt;<br /> | pTCold-MBP-(3C-TEV)-PKARIIb&lt;br&gt;<br /> | BL21(DE3)Star, pRare2-pLysS &lt;br&gt;<br /> | 5ml,25C<br /> 0.2 mM IPTG RT o/n&lt;br&gt;<br /> <br /> | yes&lt;br&gt;<br /> | &lt;br&gt;<br /> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]&lt;br&gt;<br /> | 2013/7/30<br /> yingying&lt;br&gt;<br /> <br /> |-<br /> | [[1782-pT7A3-Tth-Ligase|1782]]&lt;br&gt;<br /> | Tth-Ligase &lt;br&gt;<br /> | pT7A3-Tth-Ligase &lt;br&gt;<br /> | Nico21(DE3)plysN&lt;br&gt;<br /> | 5ml,25C<br /> 0.2 mM IPTG RT o/n&lt;br&gt;<br /> <br /> | yes&lt;br&gt;<br /> | &lt;br&gt;<br /> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]&lt;br&gt;<br /> | 2013/7/30<br /> yingying&lt;br&gt;<br /> <br /> |-<br /> | [[1797-pCold-6His-3C-MBP|1797]]&lt;br&gt;<br /> | 3C-MBP&lt;br&gt;<br /> | pCold-6His-3C-MBP&lt;br&gt;<br /> | BL21(DE3)Star, pRare2-pLysS &lt;br&gt;<br /> | 5ml,25C<br /> 0.2 mM IPTG RT o/n&lt;br&gt;<br /> <br /> | MBP and 3C seperated&lt;br&gt;<br /> | &lt;br&gt;<br /> | [[631_1497_1542_1719_1748_1765_1780_1781_1782_1797]]&lt;br&gt;<br /> | 2013/7/30<br /> yingying&lt;br&gt;<br /> <br /> |-<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> | &lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Protein_Expression]]</div> Paul.xu