https://eln.excellgen.com/index.php?action=history&feed=atom&title=Protein_Expression_Clone_Screening_2018 Protein Expression Clone Screening 2018 - Revision history 2026-10-18T09:29:33Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Protein_Expression_Clone_Screening_2018&diff=19410&oldid=prev Crm at 19:25, 10 October 2018 2018-10-10T19:25:25Z <p></p> <p><b>New page</b></p><div>Protein Expression Clone Screening<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width: 774px;&quot;<br /> |-<br /> | Clone ID<br /> | Protein Name<br /> | Host<br /> | style=&quot;width: 134px;&quot; | Condition<br /> | style=&quot;width: 138px;&quot; | Pellet after lysis<br /> | style=&quot;width: 138px;&quot; | Expression level<br /> | style=&quot;width: 56px;&quot; | Solubility<br /> | style=&quot;width: 146px;&quot; | Gel image<br /> | style=&quot;width: 33px;&quot; | Date<br /> | style=&quot;width: 28px;&quot; | By<br /> |-<br /> | 1000<br /> | FnCpf1<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.5mM IPTG, induce 4h, 37oC, OD value is around 0.8.&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | sticky<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | Y<br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_8_10_2018|Protein_QC_8_10_2018]]<br /> | style=&quot;width: 33px;&quot; | 8/10<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 942<br /> | MBP-VVN<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.5mM IPTG, induce 4h 37oC, OD value is around 0.8.&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | sticky<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | Y<br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_8_10_2018|Protein_QC_8_10_2018]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 8/10&lt;br/&gt;<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 938<br /> | PNGase F&lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.5mM IPTG, induce 4h 37oC, OD value is around 0.8.&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | sticky<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | &amp;nbsp;?<br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_8_10_2018|Protein_QC_8_10_2018]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 8/10&lt;br/&gt;<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 939<br /> | Tte-ExoIII&lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.5mM IPTG, induce 4h 37oC, OD value is around 0.8.&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | sticky<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | Y<br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_8_10_2018|Protein_QC_8_10_2018]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 8/10&lt;br/&gt;<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 941<br /> | MBP-T7-Endo&lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.5mM IPTG, induce 4h 37oC, OD value is around 0.8.&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | sticky<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | &amp;nbsp;?<br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_8_10_2018|Protein_QC_8_10_2018]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 8/10&lt;br/&gt;<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 3197<br /> | GST-BCMA&lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.5mM IPTG, induce 4h 37oC, OD value is around 0.8.&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | sticky<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | Y<br /> | style=&quot;width: 146px;&quot; | <br /> [[Protein_QC_8_10_2018|Protein_QC_8_10_2018]],<br /> <br /> [[Protein_QC_8_14_2018|Protein QC 8 14 2018]]<br /> <br /> | style=&quot;width: 33px;&quot; | 8/10&lt;br/&gt;<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 994<br /> | GST-CD133-EC2&lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.5mM IPTG, induce 4 hours at 37oC, OD value is around 0.8.&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | sticky<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 146px;&quot; | <br /> [[Protein_QC_8_10_2018|Protein_QC_8_10_2018]],<br /> <br /> [[Protein_QC_8_14_2018|Protein QC 8 14 2018]]<br /> <br /> | style=&quot;width: 33px;&quot; | 8/10&lt;br/&gt;<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 995<br /> | GST-CD133-EC3&lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.5mM IPTG, induce 4 hours at 37oC, OD value is around 0.8.&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | not sticky<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 146px;&quot; | <br /> [[Protein_QC_8_10_2018|Protein_QC_8_10_2018]],<br /> <br /> [[Protein_QC_8_14_2018|Protein QC 8 14 2018]]<br /> <br /> | style=&quot;width: 33px;&quot; | 8/10&lt;br/&gt;<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 3225<br /> | GST-MICA&lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.5mM IPTG, induce 4 h37oC, OD value is around 0.8.&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | not sticky<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | Y<br /> | style=&quot;width: 146px;&quot; | <br /> [[Protein_QC_8_10_2018|Protein_QC_8_10_2018]],<br /> <br /> [[Protein_QC_8_14_2018|Protein QC 8 14 2018]]<br /> <br /> | style=&quot;width: 33px;&quot; | 8/10&lt;br/&gt;<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 3210<br /> | Sumo-Ov-ASP1-BCMA&lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.5mM IPTG, induce 4 h 37oC, OD value is around 0.8.&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | sticky<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | Y<br /> | style=&quot;width: 146px;&quot; | <br /> [[Protein_QC_8_10_2018|Protein_QC_8_10_2018]],<br /> <br /> [[Protein_QC_8_14_2018|Protein QC 8 14 2018]]<br /> <br /> | style=&quot;width: 33px;&quot; | 8/10&lt;br/&gt;<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 3218<br /> | ULP1&lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.25mM IPTG,RT,o/n<br /> | style=&quot;width: 138px;&quot; | not sticky<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | ?<br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_8_14_2018|Protein QC 8 14 2018]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 8/14<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 3191<br /> | GST-Avitag&lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.25mM IPTG,RT,o/n&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | not sticky<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | Y<br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_8_14_2018|Protein QC 8 14 2018]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 8/14<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 824<br /> | VVTopo&lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.25mM IPTG,RT,o/n&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | sticky<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | Y<br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_8_14_2018|Protein QC 8 14 2018]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 8/14<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 999<br /> | SssI&lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.25mM IPTG,RT,o/n&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | sticky<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | N<br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_8_14_2018|Protein QC 8 14 2018]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 8/14<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 944<br /> | pfu-exo-&lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.25mM IPTG,RT,o/n&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | sticky<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | <br /> Y<br /> <br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_8_14_2018|Protein QC 8 14 2018]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 8/14<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 993<br /> | &lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 1mM IPTG,4oC,o/n&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | loose<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_4_23_18|Protein QC 4 23 18]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 4/23<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 992<br /> | &lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 1mM IPTG,4oC,o/n&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | loose<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_4_23_18|Protein QC 4 23 18]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 4/23<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 991<br /> | &lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 1mM IPTG,4oC,o/n&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | loose<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_4_23_18|Protein QC 4 23 18]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 4/23<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 990<br /> | &lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 1mM IPTG,4oC,o/n&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | loose<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_4_23_18|Protein QC 4 23 18]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 4/23<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 989<br /> | &lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 1mMIPTG,4oC,o/n&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | loose<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_4_23_18|Protein QC 4 23 18]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 4/23<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 947<br /> | &lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 1mM IPTG,4oC,o/n<br /> | style=&quot;width: 138px;&quot; | loose<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_4_23_18|Protein QC 4 23 18]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 4/23<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 986<br /> | &lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 1mM IPTG,4oC,o/n&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | loose<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_4_23_18|Protein QC 4 23 18]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 4/23<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 945<br /> | &lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 1mM IPTG,4oC,o/n&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | loose<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_4_23_18|Protein QC 4 23 18]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 4/23<br /> | style=&quot;width: 28px;&quot; | yijia<br /> |-<br /> | 942<br /> | &lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 1mM IPTG,4oC,o/n<br /> | style=&quot;width: 138px;&quot; | loose<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_5_3_2018|Protein QC 5 3 2018]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 5/3<br /> | style=&quot;width: 28px;&quot; | yijia&lt;br/&gt;<br /> |-<br /> | 944<br /> | &lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 1mM IPTG,4oC,o/n<br /> | style=&quot;width: 138px;&quot; | loose<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 146px;&quot; | [[Protein_QC_5_3_2018|Protein QC 5 3 2018]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 5/3<br /> | style=&quot;width: 28px;&quot; | yijia&lt;br/&gt;<br /> |-<br /> | 3122<br /> | CRM197&lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.25mM IPTG,RT,o/n&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | loose<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 146px;&quot; | [[Protein_Expression_8_17_2018|Protein Expression 8 17 2018]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 8/17<br /> | style=&quot;width: 28px;&quot; | yijia&lt;br/&gt;<br /> |-<br /> | 3127<br /> | qBeta-qBeta&lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.25mM IPTG,RT,o/n&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | loose<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 146px;&quot; | [[Protein_Expression_8_17_2018|Protein Expression 8 17 2018]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 8/17<br /> | style=&quot;width: 28px;&quot; | yijia&lt;br/&gt;<br /> |-<br /> | 984<br /> | SBP-SUMO-3C&lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.25mM IPTG,RT,o/n&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | loose<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 146px;&quot; | [[Protein_Expression_8_17_2018|Protein Expression 8 17 2018]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 8/17<br /> | style=&quot;width: 28px;&quot; | yijia&lt;br/&gt;<br /> |-<br /> | 3248<br /> | T7-RNAP-P266L&lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.25mM IPTG,RT,o/n&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | loose<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 146px;&quot; | [[Protein_Expression_8_17_2018|Protein Expression 8 17 2018]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 8/17<br /> | style=&quot;width: 28px;&quot; | yijia&lt;br/&gt;<br /> |-<br /> | 129<br /> | &lt;br/&gt;<br /> | Nico21(DE3)plysN&lt;br/&gt;<br /> | style=&quot;width: 134px;&quot; | 0.25mM IPTG,RT,o/n&lt;br/&gt;<br /> | style=&quot;width: 138px;&quot; | loose<br /> | style=&quot;width: 138px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 56px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 146px;&quot; | [[Protein_Expression_8_17_2018|Protein Expression 8 17 2018]]&lt;br/&gt;<br /> | style=&quot;width: 33px;&quot; | 8/17<br /> | style=&quot;width: 28px;&quot; | yijia&lt;br/&gt;<br /> |}<br /> [[Category:Protein Expression]]</div> Crm