https://eln.excellgen.com/index.php?action=history&feed=atom&title=Protein_Expression_Tasks Protein Expression Tasks - Revision history 2026-10-18T07:55:16Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Protein_Expression_Tasks&diff=20272&oldid=prev Paul.xu at 00:38, 14 October 2019 2019-10-14T00:38:41Z <p></p> <p><b>New page</b></p><div>{| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &#039;&#039;&#039;Status&#039;&#039;&#039;<br /> | style=&quot;width: 42px;&quot; | &#039;&#039;&#039;Clone ID&#039;&#039;&#039;<br /> | style=&quot;width: 36px;&quot; | &#039;&#039;&#039;Name&#039;&#039;&#039;<br /> | &#039;&#039;&#039;MW, PI&#039;&#039;&#039;&lt;br/&gt;<br /> | &#039;&#039;&#039;Host&#039;&#039;&#039;&lt;br/&gt;<br /> | &#039;&#039;&#039;&amp;nbsp;Antibiotics&#039;&#039;&#039;&lt;br/&gt;<br /> | &#039;&#039;&#039;Inducer&amp;nbsp;&#039;&#039;&#039;&lt;br/&gt;<br /> | &#039;&#039;&#039;Method/Protocol&#039;&#039;&#039;&lt;br/&gt;<br /> | &#039;&#039;&#039;Date&#039;&#039;&#039;&lt;br/&gt;<br /> | &#039;&#039;&#039;Note&#039;&#039;&#039;&lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 1078&lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | &amp;nbsp;pET28b-scIHF2&lt;br/&gt;<br /> | 9.60 / 22.8&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | <br /> no expression before<br /> <br /> EG-39,&amp;nbsp;1 F11,&amp;nbsp;1 G10<br /> <br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 1203<br /> | style=&quot;width: 36px;&quot; | pET42b-scIHF2&lt;br/&gt;<br /> | &amp;nbsp;8.38 / 54.2&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | EG-39,&amp;nbsp;no expression before,&amp;nbsp;9 B2&lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 1074&lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | pET28b-IHFab&lt;br/&gt;<br /> | 9.20 / 25.3&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | unknow expression,&amp;nbsp;1 A8,&amp;nbsp;1 B9<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 1149&lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | &amp;nbsp;pT7A3E-6H-HC-Xis&lt;br/&gt;<br /> | 10.93 / 10&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | EG-37, inclusion body,&amp;nbsp;2 I7&lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | &lt;span style=&quot;color:#FF0000;&quot;&gt;1186&lt;/span&gt;&lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | &lt;span style=&quot;color:#FF0000;&quot;&gt;pDB.His.MBP-Xis&lt;/span&gt;&lt;br/&gt;<br /> | 6.62 / 52.1&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | EG-37, low expression&amp;nbsp;[http://www.excellgen.net/eln/index.php/1143-1151-1186-screening http://www.excellgen.net/eln/index.php/1143-1151-1186-screening],&amp;nbsp;2 F4&lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 407<br /> | style=&quot;width: 36px;&quot; | pET28b-Xis&lt;br/&gt;<br /> | 11.34 / 10.7&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | EG-37&lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 406&lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | pET28b-Int&lt;br/&gt;<br /> | 9.53 / 42.5&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | don&#039;t know if there is any expression,&amp;nbsp;38 D3<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 1185&lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | pDB.His.MBP-Int&lt;br/&gt;<br /> | 7.33 / 83.7&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | 2 F3&lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 3105&lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | &amp;nbsp;pET28b-AaLS&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 3127&lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | pET28b-qBeta-qBeta-6XHis&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 3098&lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | &amp;nbsp;pET28b-AP205&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | need to check the expression of BamH1 digested/religated clone&amp;nbsp;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 812&lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | &amp;nbsp;pET30b-phoA&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 938<br /> | style=&quot;width: 36px;&quot; | PNGase F,&amp;nbsp;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 939<br /> | style=&quot;width: 36px;&quot; | Tte-Exo III<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 942<br /> | style=&quot;width: 36px;&quot; | VVN endonuclease<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 3122<br /> | style=&quot;width: 36px;&quot; | Crm197, secreted<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 931<br /> | style=&quot;width: 36px;&quot; | PAK16D ligase<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | done<br /> | style=&quot;width: 42px;&quot; | 945&lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | pT7A30-pMBP&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | done<br /> | style=&quot;width: 42px;&quot; | 947&lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | pT7A30-6His-pMBP&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 944<br /> | style=&quot;width: 36px;&quot; | pET30b-pfu-exo-&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 824&lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | pET28b-VVTopo&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | &lt;span style=&quot;color:#FF0000;&quot;&gt;3107 pFastbac-Cel1&lt;/span&gt;&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 1868&lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | &lt;span style=&quot;color: rgb(0, 0, 0); font-family: Arial, Verdana, sans-serif; font-size: 12px; white-space: nowrap;&quot;&gt;pT7A30-fMMLV&lt;/span&gt;&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | &lt;span style=&quot;color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px;&quot;&gt;1747&lt;/span&gt;&lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | &lt;span style=&quot;color: rgb(37, 37, 37); font-family: sans-serif; font-size: 14px;&quot;&gt;pT7A30-iMMLV&lt;/span&gt;&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 1483&amp;nbsp; &amp;nbsp;&lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | pT7A3-T4_DNA_Ligase&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | 927<br /> | style=&quot;width: 36px;&quot; | pET28b-Cid1<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | no expression<br /> |-<br /> | style=&quot;width: 38px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 42px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 36px;&quot; | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |}<br /> [[Category:To Do]] [[Category:Tasks]]</div> Paul.xu